Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   BEN74_RS09290 Genome accession   NZ_CP032279
Coordinates   1595461..1596207 (-) Length   248 a.a.
NCBI ID   WP_068912896.1    Uniprot ID   A0A3B7PTH8
Organism   Acinetobacter sp. WCHAc010034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1590461..1601207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN74_RS09270 (BEN74_09270) aroB 1591064..1592143 (-) 1080 WP_068913002.1 3-dehydroquinate synthase -
  BEN74_RS09275 (BEN74_09275) aroK 1592161..1592706 (-) 546 WP_068912890.1 shikimate kinase AroK -
  BEN74_RS09280 (BEN74_09280) pilQ 1592743..1594872 (-) 2130 WP_416240769.1 type IV pilus secretin PilQ Machinery gene
  BEN74_RS09285 (BEN74_09285) pilP 1594934..1595461 (-) 528 WP_068912893.1 pilus assembly protein PilP Machinery gene
  BEN74_RS09290 (BEN74_09290) pilO 1595461..1596207 (-) 747 WP_068912896.1 type 4a pilus biogenesis protein PilO Machinery gene
  BEN74_RS09295 (BEN74_09295) pilN 1596204..1596869 (-) 666 WP_068912899.1 PilN domain-containing protein Machinery gene
  BEN74_RS09300 (BEN74_09300) comM 1596869..1597891 (-) 1023 WP_068912902.1 pilus assembly protein PilM Machinery gene
  BEN74_RS09305 (BEN74_09305) ponA 1598091..1600631 (+) 2541 WP_068912904.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 27481.12 Da        Isoelectric Point: 4.7609

>NTDB_id=314702 BEN74_RS09290 WP_068912896.1 1595461..1596207(-) (pilO) [Acinetobacter sp. WCHAc010034]
MSYDEFEGLNKEAAALPKKKMTLDKFLQQFNTLDTNNYGSWPLSVKLTCWIFIIILVLLLGYFAVIKGAMDSISNANAQE
QNLLNEFREKDSKLRNLQQYQMQLQEMEARFNQQLEQLPKETEIPGLVEDINVTGVNSGLKFKNIRLENEVKQEFFIEQP
IAIEATGDYHSFGAFVSGIAGLSRIVTLHDFEITASENQEKKTDIPKVDYSLKAKTYRYVGNAENAAGAAPAASGSQAAA
AAPAGGAN

Nucleotide


Download         Length: 747 bp        

>NTDB_id=314702 BEN74_RS09290 WP_068912896.1 1595461..1596207(-) (pilO) [Acinetobacter sp. WCHAc010034]
ATGAGTTACGACGAATTTGAAGGCTTGAATAAAGAAGCCGCAGCGCTGCCAAAGAAAAAAATGACCTTGGATAAATTCCT
GCAGCAGTTCAATACCTTAGATACCAACAATTACGGCAGCTGGCCGCTGTCGGTTAAGCTCACCTGCTGGATTTTCATCA
TTATTCTGGTGCTGCTGCTGGGCTACTTTGCCGTGATTAAGGGCGCGATGGATTCGATTTCCAACGCCAATGCGCAGGAA
CAGAATTTATTGAATGAATTCAGGGAAAAAGACTCCAAGCTCCGCAACCTGCAGCAGTACCAGATGCAGCTTCAGGAAAT
GGAAGCCCGCTTCAATCAGCAGCTGGAGCAGCTGCCGAAGGAAACTGAAATTCCTGGACTTGTTGAAGACATTAACGTGA
CAGGCGTGAATTCAGGGCTGAAATTCAAGAATATCCGCCTGGAAAATGAGGTGAAGCAGGAATTCTTTATCGAGCAGCCG
ATCGCGATTGAGGCAACAGGCGATTATCACTCCTTTGGCGCCTTTGTCAGCGGTATTGCCGGATTATCCCGCATTGTGAC
TTTGCATGATTTTGAAATCACCGCATCAGAAAACCAAGAGAAAAAAACCGATATACCTAAGGTTGACTACAGCCTGAAAG
CGAAAACTTACCGCTATGTCGGCAATGCTGAAAATGCAGCGGGCGCCGCGCCGGCAGCCTCAGGAAGTCAGGCTGCGGCA
GCTGCCCCAGCAGGAGGAGCAAACTGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7PTH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

70.612

98.79

0.698
  comO Acinetobacter baylyi ADP1

68.421

99.597

0.681

Multiple sequence alignment