Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   BEN74_RS09295 Genome accession   NZ_CP032279
Coordinates   1596204..1596869 (-) Length   221 a.a.
NCBI ID   WP_068912899.1    Uniprot ID   A0A3B7PS22
Organism   Acinetobacter sp. WCHAc010034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1591204..1601869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN74_RS09275 (BEN74_09275) aroK 1592161..1592706 (-) 546 WP_068912890.1 shikimate kinase AroK -
  BEN74_RS09280 (BEN74_09280) pilQ 1592743..1594872 (-) 2130 WP_416240769.1 type IV pilus secretin PilQ Machinery gene
  BEN74_RS09285 (BEN74_09285) pilP 1594934..1595461 (-) 528 WP_068912893.1 pilus assembly protein PilP Machinery gene
  BEN74_RS09290 (BEN74_09290) pilO 1595461..1596207 (-) 747 WP_068912896.1 type 4a pilus biogenesis protein PilO Machinery gene
  BEN74_RS09295 (BEN74_09295) pilN 1596204..1596869 (-) 666 WP_068912899.1 PilN domain-containing protein Machinery gene
  BEN74_RS09300 (BEN74_09300) comM 1596869..1597891 (-) 1023 WP_068912902.1 pilus assembly protein PilM Machinery gene
  BEN74_RS09305 (BEN74_09305) ponA 1598091..1600631 (+) 2541 WP_068912904.1 penicillin-binding protein PBP1a -
  BEN74_RS09310 (BEN74_09310) - 1600862..1601665 (-) 804 WP_068913004.1 putative RNA methyltransferase -

Sequence

Protein


Download         Length: 221 a.a.        Molecular weight: 24445.96 Da        Isoelectric Point: 4.9781

>NTDB_id=314703 BEN74_RS09295 WP_068912899.1 1596204..1596869(-) (pilN) [Acinetobacter sp. WCHAc010034]
MAKINLLPWRDELREKRKKEFIAVCIGAFFIGVLGVGATWLFFDNKLQDQEQANQLIVSTNQNLDVQLKSLEGLQEQRNA
IIERMKLIQGLQGQRPIAVHLLDELVRVTPSNMYITKFSRLGDKFTLEGKAESPNTVAELLRNLEASPWYRNAFMNSFLV
AEEKKDQAPSSVVPRVEEGYGVFVVTADLDAVAEPELTGQADAKAAVPAANTATSAAEAAK

Nucleotide


Download         Length: 666 bp        

>NTDB_id=314703 BEN74_RS09295 WP_068912899.1 1596204..1596869(-) (pilN) [Acinetobacter sp. WCHAc010034]
ATGGCGAAAATAAACCTGCTCCCTTGGCGCGATGAGCTGAGGGAGAAACGGAAAAAAGAATTCATCGCAGTCTGCATCGG
GGCTTTTTTTATCGGGGTACTCGGGGTAGGTGCAACCTGGCTATTCTTTGACAACAAGCTGCAGGATCAGGAACAGGCGA
ATCAGCTGATCGTCAGCACCAATCAGAATTTGGATGTGCAGCTGAAGTCGCTGGAAGGCCTGCAGGAGCAGCGCAATGCC
ATTATAGAGCGCATGAAGCTGATTCAGGGCCTGCAGGGCCAGCGCCCAATCGCCGTGCATCTGCTTGATGAGCTGGTCCG
CGTCACGCCAAGCAATATGTATATCACTAAATTCAGCCGCTTAGGCGATAAGTTTACCCTGGAAGGCAAAGCTGAAAGCC
CGAATACTGTTGCGGAGCTGCTGCGCAATCTTGAAGCATCGCCTTGGTACCGCAATGCATTCATGAATTCGTTCCTGGTG
GCGGAAGAGAAAAAGGATCAGGCGCCAAGTTCAGTGGTTCCACGGGTTGAAGAAGGCTATGGCGTTTTTGTGGTTACAGC
GGACTTGGACGCGGTTGCCGAGCCGGAATTAACAGGCCAGGCGGATGCTAAGGCTGCTGTCCCGGCAGCAAATACAGCAA
CTTCTGCAGCGGAGGCGGCAAAATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7PS22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

69.266

98.643

0.683
  comN Acinetobacter baylyi ADP1

74.074

85.52

0.633

Multiple sequence alignment