Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D0907_RS13755 Genome accession   NZ_CP032090
Coordinates   3057647..3059323 (+) Length   558 a.a.
NCBI ID   WP_118844625.1    Uniprot ID   -
Organism   Pseudoalteromonas lipolytica strain HJ51     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3052647..3064323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0907_RS13725 (D0907_13725) - 3052950..3053390 (+) 441 WP_118844620.1 pilin -
  D0907_RS21220 (D0907_13730) - 3053445..3053834 (+) 390 WP_279636386.1 pilin -
  D0907_RS13735 (D0907_13735) - 3053831..3054439 (-) 609 WP_118844822.1 fimbrial assembly protein -
  D0907_RS13740 (D0907_13740) pilC 3054571..3055797 (+) 1227 WP_118844622.1 type II secretion system F family protein Machinery gene
  D0907_RS13745 (D0907_13745) pilD 3055977..3056873 (+) 897 WP_118844623.1 prepilin peptidase Machinery gene
  D0907_RS13750 (D0907_13750) coaE 3056876..3057472 (+) 597 WP_118844624.1 dephospho-CoA kinase -
  D0907_RS13755 (D0907_13755) pilB 3057647..3059323 (+) 1677 WP_118844625.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0907_RS13760 (D0907_13760) yacG 3059391..3059624 (+) 234 WP_036965896.1 DNA gyrase inhibitor YacG -
  D0907_RS13765 (D0907_13765) - 3059686..3060216 (-) 531 WP_118844626.1 Spy/CpxP family protein refolding chaperone -
  D0907_RS13770 (D0907_13770) - 3060388..3061077 (+) 690 WP_118844627.1 response regulator -
  D0907_RS13775 (D0907_13775) - 3061079..3062464 (+) 1386 WP_118844628.1 ATP-binding protein -
  D0907_RS13780 (D0907_13780) - 3062465..3063406 (+) 942 WP_036965889.1 alpha/beta fold hydrolase -
  D0907_RS13785 (D0907_13785) - 3063385..3064293 (-) 909 WP_074988960.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62244.50 Da        Isoelectric Point: 5.4641

>NTDB_id=313674 D0907_RS13755 WP_118844625.1 3057647..3059323(+) (pilB) [Pseudoalteromonas lipolytica strain HJ51]
MNINSPLLRKFITLGRIDAETVKLKQREYNSTAELISKSCGIDAHELAEQCTDLFRVPYFDIKDFDVSLISDELVKEKLI
REHHILPLIKKGRKLYIAASDPTDYGAFENFEFSTGLSCEVVVVDYNQLENKIEQLLDAAGSLHLTDDEFKELGDLDTDK
PRDMPSNDEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHRYRVRFRIDGILHEMASPPNSLASRLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALKAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEKLPDEELIRQGFDPATLSKLTL
FSPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKSGFNNLRLSGLKKAADGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=313674 D0907_RS13755 WP_118844625.1 3057647..3059323(+) (pilB) [Pseudoalteromonas lipolytica strain HJ51]
ATGAATATTAACTCACCGTTACTGAGAAAATTTATCACATTAGGTCGCATTGATGCAGAGACCGTCAAATTAAAGCAACG
AGAATATAACTCCACAGCTGAGCTCATTTCTAAGAGCTGCGGTATAGACGCGCATGAATTAGCAGAGCAATGCACTGACT
TGTTCCGTGTTCCCTATTTTGATATCAAAGACTTCGATGTATCACTTATTTCTGATGAGTTAGTAAAAGAAAAGCTCATT
CGCGAGCATCATATCCTCCCGCTGATAAAGAAAGGCCGTAAGCTCTATATAGCGGCATCGGACCCTACTGATTACGGTGC
GTTTGAAAACTTTGAATTTAGTACAGGTCTGTCTTGTGAAGTTGTAGTGGTTGATTACAACCAGCTAGAAAATAAAATAG
AGCAGCTGCTCGATGCGGCCGGTAGCTTACATTTAACGGATGACGAATTTAAAGAGCTTGGCGATCTCGACACCGATAAA
CCACGCGATATGCCAAGTAATGATGAAGACAAAGACGATGCGCCTATTATTGTCTACATCAATAAGATTTTGATGGATGC
AATAAAAAAAGGGGCATCAGATTTACACTTCGAGCCATACGAGCATCGTTATCGAGTGCGCTTTCGGATTGATGGCATTT
TACACGAAATGGCAAGCCCACCGAATAGCCTAGCAAGTCGATTATCTGCGCGCATAAAAGTTATGTCACGCTTAGATATC
GCCGAAAAACGTAAACCACAAGATGGTCGCATTAAATTAAAAATTACTGAACGTAAAAGTATCGACTTTCGTGTCAGTAC
CCTGCCAACGCTGTGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCAAGTGCTATGCTCGGCATCGATGTATTGG
GTTATGAGCCTGAACAGAAAAAGCTCTACATGGATGCGCTCGAACAGCCGCAAGGGATGATACTAGTGACAGGCCCTACA
GGCTCTGGTAAAACAGTATCTCTTTACACCGGCTTGAATATTTTGAACCAACCTGAGCGCAATATTAGTACTGCGGAAGA
TCCGGTTGAGATCAACCTTGAAGGCATTAACCAAGTACAAATTAACACAAAAGCAGATATGACGTTCGCCAACGCGTTAA
AAGCGTTTTTGCGTCAAGATCCCGATGTCGTCATGGTCGGTGAGATACGAGATCTAGAAACAGCTGAAATTTCCATTAAA
GCAGCGCAAACAGGCCACCTAGTCTTATCGACACTGCACACAAATTCAGCGCCAGAAACACTGACTCGATTGTTAAACAT
GGGCGTTCCGGCATACAACGTTGCAAGTTCAATTAGTCTTATCATTGCACAGCGACTGGCCAGACGTTTATGTCCGAAAT
GTAAAACCCCAGAAAAACTGCCCGATGAAGAACTTATTCGCCAAGGCTTTGACCCCGCAACATTGAGTAAACTCACGCTG
TTTTCTCCAAAAGGCTGTGATAGCTGTACCGATGGCTATAAGGGACGAGTAGGTATTTACGAAGTCATGCAAATTACCCC
TGAAATTGCGCAAATTATTATGCGTGGGGGTAATTCACTCGAAATTGCTGAGGTTTCTTTAAAATCAGGTTTTAATAACT
TACGTCTATCGGGATTAAAAAAAGCGGCGGATGGTGTCACCTCGCTAGCTGAAATAAATCGGGTCACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.371

100

0.579
  pilB Acinetobacter baylyi ADP1

55.536

100

0.557
  pilB Legionella pneumophila strain ERS1305867

53.789

96.953

0.522
  pilB Vibrio cholerae strain A1552

54.023

93.548

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

52.247

95.699

0.5
  pilB Vibrio campbellii strain DS40M4

52.06

95.699

0.498
  pilF Neisseria gonorrhoeae MS11

49.423

93.19

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.772

93.369

0.362

Multiple sequence alignment