Detailed information NTG0313443

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A320_RS17185
• Genome accession: NZ_CP032055
• Coordinates: 3603554..3604612 (-)
• Length: 352 a.a.
• NCBI ID: WP_004644147.1
• Uniprot ID: A0A7U3Y305
• Organism: Acinetobacter baumannii strain A320 (RUH134)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3598554..3609612

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A320_RS17160 (A320_03378)	aroK	3598829..3599398 (-)	570	WP_000643712.1	shikimate kinase AroK	-
A320_RS17165 (A320_03379)	pilQ	3599410..3601575 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
A320_RS17170 (A320_03380)	pilP	3601638..3602165 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
A320_RS17175 (A320_03381)	pilO	3602176..3602916 (-)	741	WP_000076099.1	type 4a pilus biogenesis protein PilO	Machinery gene
A320_RS17180 (A320_03382)	pilN	3602913..3603554 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
A320_RS17185 (A320_03383)	pilM	3603554..3604612 (-)	1059	WP_004644147.1	pilus assembly protein PilM	Machinery gene
A320_RS17190 (A320_03384)	ponA	3604774..3607329 (+)	2556	WP_000736667.1	penicillin-binding protein PBP1a	-
A320_RS17195 (A320_03385)	-	3607347..3608171 (-)	825	WP_002022249.1	putative RNA methyltransferase	-
A320_RS17200 (A320_03386)	-	3608174..3608506 (-)	333	WP_000169602.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39062.61 Da
• Isoelectric Point: 4.4618

>NTDB_id=313443 A320_RS17185 WP_004644147.1 3603554..3604612(-) (pilM) [Acinetobacter baumannii strain A320 (RUH134)]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLTNPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=313443 A320_RS17185 WP_004644147.1 3603554..3604612(-) (pilM) [Acinetobacter baumannii strain A320 (RUH134)]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTA
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A7U3Y305

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	99.716	100	0.997
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423