Detailed information NTG0311856

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: D0851_RS16165
• Genome accession: NZ_CP031848
• Coordinates: 3439796..3440860 (+)
• Length: 354 a.a.
• NCBI ID: WP_117619546.1
• Uniprot ID: A0A346S493
• Organism: Marinobacter sp. Arc7-DN-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3434796..3445860

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D0851_RS16150 (D0851_16150)	rpmE	3435300..3435521 (+)	222	WP_117619543.1	50S ribosomal protein L31	-
D0851_RS16155 (D0851_16155)	-	3435738..3437003 (+)	1266	WP_117619544.1	malic enzyme-like NAD(P)-binding protein	-
D0851_RS16160 (D0851_16160)	-	3437075..3439600 (-)	2526	WP_117619545.1	penicillin-binding protein 1A	-
D0851_RS16165 (D0851_16165)	pilM	3439796..3440860 (+)	1065	WP_117619546.1	pilus assembly protein PilM	Machinery gene
D0851_RS16170 (D0851_16170)	-	3440860..3441423 (+)	564	WP_117619547.1	PilN domain-containing protein	-
D0851_RS16175 (D0851_16175)	-	3441423..3442034 (+)	612	WP_117619548.1	type 4a pilus biogenesis protein PilO	-
D0851_RS16180 (D0851_16180)	-	3442037..3442585 (+)	549	WP_117619549.1	pilus assembly protein PilP	-
D0851_RS16185 (D0851_16185)	pilQ	3442651..3444732 (+)	2082	WP_205422227.1	type IV pilus secretin PilQ	Machinery gene
D0851_RS16190 (D0851_16190)	aroK	3444871..3445437 (+)	567	WP_117619551.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37901.96 Da
• Isoelectric Point: 4.1452

>NTDB_id=311856 D0851_RS16165 WP_117619546.1 3439796..3440860(+) (pilM) [Marinobacter sp. Arc7-DN-1]
MFGLLGKKSSAVLGVDISSSSVKLLELSKQGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTSVKQVAV
AVSGSAVITKVIQMDGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSESNPDQVDVLLAACRKENVDIREDALEI
ASLTTKVVDVEAYSLERAYALIEPQLDSQGEELVVAIVDIGATMTTLSVLAGGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYESEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPALVANP
FADMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=311856 D0851_RS16165 WP_117619546.1 3439796..3440860(+) (pilM) [Marinobacter sp. Arc7-DN-1]
GTGTTCGGATTGTTAGGAAAGAAATCCAGTGCAGTGCTGGGCGTGGATATAAGCTCCAGCTCGGTCAAACTTCTGGAGCT
GTCGAAGCAGGGTGACCGGTACAAGGTCGAAAGCTACGCAGTGGAGCCGTTACCGGCGAACGCTGTGGTCGAAAAAAATA
TCACGGATGTCGAGGCGGTGGGCGAAGTGCTGAAGCGCGTTGCCTCCAAGTCCCGCACCAGCGTGAAGCAGGTTGCGGTC
GCGGTCTCCGGCTCCGCGGTTATCACCAAGGTCATCCAGATGGATGGTGGCCTCAACGAGTTCGAGATGGAAGATCAGAT
CGCCCTCGAGGCAGATCAGTACATTCCATACCCGCTGGATGAGGTGGCCATCGACTTTGAGGTACAGGGTCCCTCCGAAA
GCAATCCTGATCAGGTTGATGTGCTTCTGGCCGCGTGCCGGAAAGAAAATGTCGACATACGGGAAGACGCCCTCGAGATT
GCCTCACTGACAACCAAAGTTGTTGATGTCGAAGCCTACTCACTGGAACGGGCATATGCGCTGATAGAGCCGCAGCTTGA
CTCACAGGGTGAAGAGCTGGTGGTTGCTATTGTCGACATTGGCGCAACCATGACAACACTCAGTGTCCTGGCCGGCGGAA
AGACAGTGTACACCCGGGAGCAGATTTTTGGCGGCAAACAGCTGACTGAGGAAATCCAGCGCCGGTACGGATTGTCTCTT
GAAGAGGCCGGGCTCGCCAAGAAGCAGGGTGGATTGCCCGATGACTATGAGTCAGAGGTTCTGACGCCGTTCCGTGAAGC
TGTCGTGCAGCAGGTGGCGCGGGCGCTTCAGTTCTTCTTTGGTGCCAGCCAGTATAATGCTGTCGACTATGTGGTTCTGG
CAGGCGGTACTGCCTCTATCCAGGGGCTGACTGAAATGGTCGAAGAAAAAACAGGAACACCGGCACTGGTGGCAAACCCG
TTTGCAGATATGGCAGTGGGGTCACGGGTCAATGCATCGGCGCTGAGTAATGATGCTCCCTCTCTGATGATTGCCTGCGG
CCTGGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A346S493

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.672	100	0.537
comM	Acinetobacter nosocomialis M2	53.672	100	0.537
comM	Acinetobacter baylyi ADP1	51.412	100	0.514
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46