Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CJA_RS02025 Genome accession   NC_010995
Coordinates   511345..512412 (+) Length   355 a.a.
NCBI ID   WP_012486094.1    Uniprot ID   B3PI73
Organism   Cellvibrio japonicus Ueda107     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 503656..525410 511345..512412 within 0


Gene organization within MGE regions


Location: 503656..525410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJA_RS01990 (CJA_0405) hslV 503656..504180 (-) 525 WP_012486087.1 ATP-dependent protease subunit HslV -
  CJA_RS01995 (CJA_0406) - 504270..504818 (-) 549 WP_012486088.1 SPOR domain-containing protein -
  CJA_RS02000 (CJA_0407) - 504878..507073 (-) 2196 WP_012486089.1 primosomal protein N' -
  CJA_RS02005 (CJA_0408) - 507183..507485 (+) 303 WP_041550922.1 high-potential iron-sulfur protein -
  CJA_RS02010 (CJA_0409) rpmE 507652..507870 (+) 219 WP_041550924.1 50S ribosomal protein L31 -
  CJA_RS02015 (CJA_0410) - 507875..508765 (+) 891 WP_012486092.1 thermonuclease family protein -
  CJA_RS02020 (CJA_0411) - 508779..511190 (-) 2412 WP_049765383.1 penicillin-binding protein 1A -
  CJA_RS02025 (CJA_0412) pilM 511345..512412 (+) 1068 WP_012486094.1 pilus assembly protein PilM Machinery gene
  CJA_RS02030 (CJA_0413) - 512412..513002 (+) 591 WP_012486095.1 PilN domain-containing protein -
  CJA_RS02035 (CJA_0414) - 513002..513655 (+) 654 WP_012486096.1 type 4a pilus biogenesis protein PilO -
  CJA_RS02040 (CJA_0415) - 513652..514176 (+) 525 WP_012486097.1 pilus assembly protein PilP -
  CJA_RS02045 (CJA_0416) pilQ 514183..516357 (+) 2175 WP_238526854.1 type IV pilus secretin PilQ Machinery gene
  CJA_RS02050 (CJA_0417) aroK 516358..516882 (+) 525 WP_012486099.1 shikimate kinase AroK -
  CJA_RS02055 (CJA_0418) aroB 517032..518117 (+) 1086 WP_041550928.1 3-dehydroquinate synthase -
  CJA_RS02060 (CJA_0419) - 518147..519805 (+) 1659 WP_083766814.1 SPOR domain-containing protein -
  CJA_RS02065 (CJA_0420) gcvH 519866..520258 (-) 393 WP_012486102.1 glycine cleavage system protein GcvH -
  CJA_RS02070 (CJA_0421) gcvT 520301..521416 (-) 1116 WP_012486103.1 glycine cleavage system aminomethyltransferase GcvT -
  CJA_RS02075 (CJA_0422) - 521536..522105 (-) 570 WP_012486104.1 YbhB/YbcL family Raf kinase inhibitor-like protein -
  CJA_RS02080 (CJA_0423) - 522133..523155 (-) 1023 WP_012486105.1 FHA domain-containing protein -
  CJA_RS02085 (CJA_0424) - 523152..524558 (-) 1407 WP_012486106.1 S1C family serine protease -
  CJA_RS02090 (CJA_0425) - 524652..525410 (-) 759 WP_012486107.1 ComF family protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38743.37 Da        Isoelectric Point: 4.3634

>NTDB_id=31114 CJA_RS02025 WP_012486094.1 511345..512412(+) (pilM) [Cellvibrio japonicus Ueda107]
MGILSFLDKKAKPVIGLDISSTSVKLLELTRSGDRYRVESYSVKPLPPNAVVEKNIADPVAVAEVIRTMVKQSKTKLKHA
AVAVAGSAVITKVIDMPAELNEDAMESQIAAEADQYIPFPLEEVALDFEIQGPSPRNPEQVEVLLAACRRENVEVREQVL
AESGLLAEKVDIEAYCMERAFELIAEQLEDQEGQVVAIIDIGATMTTLSVLVDGKTVYTREQLFGGRQLTEEIQRRYGLS
REEAGLAKKQGGLPDDYEMEVLNPFKDAVVQQVTRSLQFFFSASQYNDVDYIILAGGVASLEGLVGLIEEKLGTQTVVAN
PFARMSVSSRVNAVSLATDAPSLMIVTGLAMRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=31114 CJA_RS02025 WP_012486094.1 511345..512412(+) (pilM) [Cellvibrio japonicus Ueda107]
ATGGGCATTTTAAGTTTCCTAGATAAAAAGGCGAAGCCGGTGATTGGGCTGGATATCAGTTCCACGTCGGTAAAGCTGTT
AGAGCTCACGCGTAGCGGAGACCGCTATCGTGTTGAATCCTATTCCGTAAAACCCCTTCCACCCAATGCGGTGGTTGAAA
AAAATATTGCAGACCCGGTTGCTGTCGCTGAAGTCATTCGCACCATGGTCAAGCAATCCAAGACCAAGCTAAAGCATGCA
GCGGTGGCCGTGGCGGGTTCGGCGGTGATCACCAAGGTGATCGATATGCCTGCTGAGCTGAATGAAGATGCGATGGAAAG
CCAGATTGCGGCCGAAGCGGATCAATATATACCCTTTCCCCTGGAAGAGGTTGCCTTGGATTTTGAAATTCAGGGGCCAT
CCCCGCGCAACCCTGAACAGGTAGAGGTTTTACTGGCAGCCTGTCGTCGTGAAAACGTTGAAGTTCGCGAACAGGTGCTA
GCCGAGTCCGGCTTGCTTGCAGAGAAGGTGGATATTGAAGCCTACTGTATGGAGCGGGCATTCGAATTAATTGCCGAGCA
GCTTGAGGACCAGGAAGGCCAGGTGGTAGCAATTATTGATATAGGTGCCACCATGACCACACTCAGTGTGTTGGTTGATG
GCAAGACGGTATATACCCGCGAACAATTATTTGGTGGGCGCCAGCTGACAGAGGAAATCCAGCGACGTTATGGTTTGTCG
CGTGAAGAGGCCGGCCTGGCTAAAAAGCAGGGTGGTCTCCCCGATGATTATGAAATGGAAGTGCTTAACCCATTCAAGGA
TGCTGTCGTACAGCAGGTTACCCGCTCACTCCAGTTCTTTTTCTCAGCTAGCCAATATAACGATGTGGATTACATCATCC
TCGCCGGCGGTGTTGCGTCTCTGGAAGGTTTGGTTGGTTTGATTGAGGAAAAGCTGGGAACTCAAACGGTAGTTGCCAAT
CCTTTTGCGCGTATGTCGGTCTCCTCTCGTGTTAATGCGGTCTCTCTGGCTACTGATGCTCCTTCATTAATGATTGTTAC
CGGTCTGGCAATGAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B3PI73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.541

99.437

0.532
  comM Acinetobacter nosocomialis M2

53.258

99.437

0.53
  comM Acinetobacter baylyi ADP1

50.708

99.437

0.504
  pilM Legionella pneumophila strain ERS1305867

42.655

99.718

0.425

Multiple sequence alignment