Detailed information NTG0310803

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: D0C16_RS22340
• Genome accession: NZ_CP031728
• Coordinates: 5311780..5312952 (+)
• Length: 390 a.a.
• NCBI ID: WP_151034382.1
• Uniprot ID: A0A5J6PDK5
• Organism: Cellvibrio sp. KY-GH-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5306780..5317952

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D0C16_RS22315 (D0C16_22625)	-	5306826..5308070 (+)	1245	WP_151034377.1	hypothetical protein	-
D0C16_RS22320 (D0C16_22630)	-	5308128..5309282 (-)	1155	WP_225318818.1	lipid A deacylase LpxR family protein	-
D0C16_RS22325 (D0C16_22635)	-	5309493..5310083 (-)	591	WP_151034379.1	TetR/AcrR family transcriptional regulator	-
D0C16_RS22330 (D0C16_22640)	-	5310239..5311258 (-)	1020	WP_151034380.1	nucleoid-associated protein	-
D0C16_RS22335 (D0C16_22645)	-	5311360..5311710 (+)	351	WP_151034381.1	RidA family protein	-
D0C16_RS22340 (D0C16_22650)	pilU	5311780..5312952 (+)	1173	WP_151034382.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
D0C16_RS22345 (D0C16_22655)	-	5312999..5315488 (+)	2490	WP_151034383.1	bifunctional acetate--CoA ligase family protein/GNAT family N-acetyltransferase	-
D0C16_RS22350 (D0C16_22660)	-	5315532..5316380 (+)	849	WP_151034384.1	ABC transporter substrate-binding protein	-
D0C16_RS22355 (D0C16_22665)	-	5316509..5317360 (-)	852	WP_151034385.1	GTP-binding protein	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 42976.43 Da
• Isoelectric Point: 6.4252

>NTDB_id=310803 D0C16_RS22340 WP_151034382.1 5311780..5312952(+) (pilU) [Cellvibrio sp. KY-GH-1]
MATPIDQYLNILAKKDGSDLYLSTGAPPCAKFQGVLKPLTSEPFKPGEIESIANAIMDDEQRAAFTHELEMNLAISIHGV
GRFRINIFKQRNEVAIVARNIKTDIPQFDALGLPEVLKDVIMTKRGLVLFVGGTGSGKSTSLAALIDHRNSSSGGHIITI
EDPVEFVHKHKRSVINQREVGVDTRSYKAALKNTLRQAPDVILIGEIRDRETMEHALEFAETGHLAISTLHANNSNQALE
RIINLFPEERRPQLLMGLSQNLRAFVSQRLLPTVDGKRCAAVEVLLGTKTIQELILKSRLTEIKEIMEKSENLGMQTFDG
AIFKLYMAGKVSFDDAIANADSPNNLRLRIKLATDGVVVPKAEPVVAEPKAPPTSFGELSLQKMDEDEEP

Nucleotide

• Length: 1173 bp

>NTDB_id=310803 D0C16_RS22340 WP_151034382.1 5311780..5312952(+) (pilU) [Cellvibrio sp. KY-GH-1]
ATGGCAACACCTATAGATCAATATTTAAATATCCTCGCCAAAAAAGATGGTTCGGATTTGTACTTGAGTACGGGCGCGCC
GCCCTGTGCAAAATTTCAAGGCGTATTAAAACCGCTCACCAGTGAACCCTTTAAACCTGGAGAAATTGAGAGTATTGCCA
ATGCCATCATGGATGATGAGCAGCGTGCAGCCTTTACCCACGAACTGGAAATGAACCTCGCTATCTCTATTCATGGTGTA
GGTCGTTTTCGCATTAATATTTTCAAGCAGCGCAATGAAGTGGCGATTGTTGCGCGCAATATCAAAACGGATATTCCACA
ATTCGATGCGCTGGGCCTGCCGGAAGTGTTGAAAGATGTGATCATGACCAAACGTGGTTTGGTGTTGTTTGTCGGTGGCA
CCGGCTCGGGTAAGTCTACCTCCCTGGCAGCGTTGATTGATCATCGCAATTCTTCCAGCGGTGGTCACATCATCACCATC
GAAGATCCAGTGGAGTTCGTACATAAGCATAAGCGCAGTGTAATTAACCAACGTGAAGTGGGCGTGGATACGCGCAGTTA
TAAAGCGGCATTGAAAAACACTTTGCGCCAGGCTCCGGATGTCATTCTGATTGGTGAAATTCGCGATCGCGAAACCATGG
AGCATGCGTTGGAGTTTGCTGAAACCGGGCATTTAGCGATCTCAACCCTGCACGCCAATAATTCCAATCAGGCATTGGAG
CGGATCATCAATTTGTTTCCGGAAGAGCGCCGCCCGCAATTATTGATGGGGCTCTCGCAAAACTTGCGTGCATTTGTGTC
GCAGCGGTTATTGCCCACGGTAGATGGAAAGCGCTGCGCGGCGGTAGAAGTATTGCTCGGCACTAAAACAATTCAGGAAT
TGATTTTAAAAAGTCGCCTCACTGAAATTAAAGAAATTATGGAGAAATCTGAAAATCTCGGCATGCAAACTTTCGACGGT
GCGATTTTTAAATTATACATGGCTGGTAAAGTCAGTTTTGATGATGCTATCGCCAATGCCGACTCCCCCAATAATTTGCG
TTTGCGAATCAAACTGGCGACTGATGGTGTGGTTGTTCCCAAAGCAGAACCTGTTGTTGCAGAACCTAAAGCACCGCCAA
CCAGTTTTGGTGAATTGAGTTTGCAAAAAATGGATGAAGATGAAGAGCCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-246)

3D structure

• AlphaFold DB: A0A5J6PDK5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.803	91.026	0.49
pilU	Acinetobacter baylyi ADP1	51.714	89.744	0.464
pilU	Vibrio cholerae strain A1552	50.287	89.231	0.449
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.889	96.923	0.377