Detailed information NTG0310791

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: D0C16_RS13400
• Genome accession: NZ_CP031728
• Coordinates: 3127022..3128089 (-)
• Length: 355 a.a.
• NCBI ID: WP_151032848.1
• Uniprot ID: A0A5J6P8I5
• Organism: Cellvibrio sp. KY-GH-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3122022..3133089

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D0C16_RS13375 (D0C16_13605)	aroK	3122595..3123110 (-)	516	WP_151032843.1	shikimate kinase AroK	-
D0C16_RS13380 (D0C16_13610)	pilQ	3123110..3125257 (-)	2148	WP_151032844.1	type IV pilus secretin PilQ family protein	Machinery gene
D0C16_RS13385 (D0C16_13615)	-	3125294..3125818 (-)	525	WP_151032845.1	pilus assembly protein PilP	-
D0C16_RS13390 (D0C16_13620)	-	3125815..3126435 (-)	621	WP_151032846.1	type 4a pilus biogenesis protein PilO	-
D0C16_RS13395 (D0C16_13625)	-	3126435..3127022 (-)	588	WP_151032847.1	PilN domain-containing protein	-
D0C16_RS13400 (D0C16_13630)	pilM	3127022..3128089 (-)	1068	WP_151032848.1	pilus assembly protein PilM	Machinery gene
D0C16_RS13405 (D0C16_13635)	-	3128193..3130655 (+)	2463	WP_151032849.1	penicillin-binding protein 1A	-
D0C16_RS13410 (D0C16_13640)	-	3130660..3131487 (-)	828	WP_151032850.1	thermonuclease family protein	-
D0C16_RS13415 (D0C16_13645)	rpmE	3131501..3131722 (-)	222	WP_151032851.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 38694.27 Da
• Isoelectric Point: 4.3486

>NTDB_id=310791 D0C16_RS13400 WP_151032848.1 3127022..3128089(-) (pilM) [Cellvibrio sp. KY-GH-1]
MGILSFLEKKAKPVIGLDISSTSVKLLELSRSGDRYRVESYTVKALPPNAVVEKNIADPAAVAEVVRSMVKQSKTKLKHA
AVAVAGSAVITKMIDMPADLNDDAMESQIAAEADQYIPFPLEEVALDFEVQGISPRNPDQVEVLLAACRRENVEVRQQVL
ADSDLIAEKVDIEAYCMERAFELIAEQLEDQEGQVVAIIDIGATMTTLSVLVDGKTVYTREQLFGGRQLTEEIQRRYGLS
REEAGLAKKQGGLPDDYEMEVLTPFKDAVVQQVTRSLQFFFSASQYNDVDYIILAGGVASLEGLVGLIEEKLGTQTVVAN
PFARMSVSSRVNAVSLANDAPALMIVTGLAMRSFD

Nucleotide

• Length: 1068 bp

>NTDB_id=310791 D0C16_RS13400 WP_151032848.1 3127022..3128089(-) (pilM) [Cellvibrio sp. KY-GH-1]
ATGGGCATTTTAAGTTTCCTCGAAAAGAAGGCAAAGCCGGTGATTGGGTTGGATATCAGCTCAACCTCGGTCAAATTGCT
TGAACTCAGTCGCAGTGGTGATCGCTATCGCGTTGAGTCCTATACCGTAAAAGCCCTACCGCCGAACGCGGTGGTGGAGA
AAAATATTGCTGATCCGGCAGCGGTGGCTGAGGTTGTCCGCTCGATGGTCAAGCAATCCAAAACCAAGTTGAAGCATGCT
GCGGTTGCGGTCGCGGGTTCAGCGGTGATCACCAAAATGATCGATATGCCGGCGGACCTGAATGACGATGCAATGGAAAG
CCAAATCGCTGCAGAAGCTGATCAATATATTCCTTTCCCTTTAGAGGAGGTTGCTCTGGACTTTGAGGTTCAGGGGATCT
CTCCGCGCAATCCCGATCAAGTTGAAGTTCTGCTTGCTGCCTGCCGACGTGAAAATGTTGAGGTGCGTCAACAGGTGCTC
GCTGATTCAGATTTAATTGCTGAAAAAGTTGATATCGAAGCCTATTGCATGGAGCGCGCATTTGAGCTGATCGCCGAACA
GTTAGAGGATCAGGAAGGTCAGGTAGTAGCGATTATCGACATTGGCGCCACTATGACAACGTTGAGTGTGTTGGTTGATG
GGAAGACAGTCTATACGCGTGAACAATTGTTTGGCGGTCGCCAGCTTACTGAGGAAATTCAGCGTCGTTATGGGTTGTCC
CGGGAAGAGGCTGGGCTAGCTAAGAAGCAAGGTGGCCTGCCAGATGATTATGAAATGGAAGTTTTAACGCCCTTTAAAGA
TGCGGTTGTGCAGCAAGTTACTCGTTCTCTTCAATTTTTCTTTTCTGCGAGTCAGTACAACGATGTCGACTACATTATTC
TTGCCGGCGGTGTCGCATCGCTGGAAGGATTGGTCGGATTAATTGAGGAGAAATTAGGAACGCAAACGGTAGTGGCGAAT
CCGTTTGCGCGCATGTCTGTTTCATCTCGCGTTAATGCGGTGTCTTTGGCAAATGATGCCCCTGCGTTAATGATCGTCAC
TGGATTGGCAATGAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (16-354)

3D structure

• AlphaFold DB: A0A5J6P8I5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	54.674	99.437	0.544
comM	Acinetobacter nosocomialis M2	54.391	99.437	0.541
comM	Acinetobacter baylyi ADP1	51.275	99.437	0.51
pilM	Legionella pneumophila strain ERS1305867	42.655	99.718	0.425