Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   DXY21_RS12220 Genome accession   NZ_CP031694
Coordinates   2386569..2387006 (+) Length   145 a.a.
NCBI ID   WP_003153088.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM101368     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2381569..2392006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXY21_RS12195 (DXY21_02432) - 2381583..2382884 (-) 1302 WP_014305416.1 hemolysin family protein -
  DXY21_RS12200 (DXY21_02433) - 2383030..2383980 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  DXY21_RS12205 (DXY21_02434) comGA 2384172..2385242 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  DXY21_RS12210 (DXY21_02435) comGB 2385229..2386266 (+) 1038 WP_003153086.1 competence type IV pilus assembly protein ComGB Machinery gene
  DXY21_RS12215 (DXY21_02436) comGC 2386313..2386579 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  DXY21_RS12220 (DXY21_02437) comGD 2386569..2387006 (+) 438 WP_003153088.1 competence type IV pilus minor pilin ComGD Machinery gene
  DXY21_RS12225 (DXY21_02438) comGE 2386990..2387304 (+) 315 WP_003153089.1 competence type IV pilus minor pilin ComGE -
  DXY21_RS12230 (DXY21_02439) comGF 2387318..2387713 (+) 396 WP_003153090.1 competence type IV pilus minor pilin ComGF -
  DXY21_RS12235 (DXY21_02440) comGG 2387714..2388091 (+) 378 WP_003153092.1 competence type IV pilus minor pilin ComGG Machinery gene
  DXY21_RS12240 (DXY21_02441) - 2388148..2388327 (+) 180 WP_003153093.1 YqzE family protein -
  DXY21_RS12245 (DXY21_02442) - 2388367..2388696 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  DXY21_RS12250 (DXY21_02443) tapA 2388955..2389626 (+) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  DXY21_RS12255 (DXY21_02444) sipW 2389598..2390182 (+) 585 WP_003153100.1 signal peptidase I SipW -
  DXY21_RS12260 (DXY21_02445) tasA 2390246..2391031 (+) 786 WP_003153102.1 biofilm matrix protein TasA -
  DXY21_RS12265 (DXY21_02446) sinR 2391079..2391414 (-) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  DXY21_RS12270 (DXY21_02447) sinI 2391448..2391621 (-) 174 WP_003153105.1 anti-repressor SinI Regulator

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16324.85 Da        Isoelectric Point: 10.3725

>NTDB_id=310412 DXY21_RS12220 WP_003153088.1 2386569..2387006(+) (comGD) [Bacillus velezensis strain SRCM101368]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPREHKYK
LQSAGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIRLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=310412 DXY21_RS12220 WP_003153088.1 2386569..2387006(+) (comGD) [Bacillus velezensis strain SRCM101368]
TTGAACAATAACAGGCGGACAGAAAACGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTTCAAAAAGATA
TTCAGCTTGCGCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAGAGAGCATAAATACAAA
CTGCAGTCAGCCGGAAGGATTGTCGAGCGTTCTTTTGATTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCGATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

54.795

100

0.552

Multiple sequence alignment