Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EW646_RS22050 Genome accession   NZ_CP035770
Coordinates   4447978..4448646 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O145 strain RM8988-C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4442978..4453646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW646_RS22020 (EW646_22580) rfaQ 4443156..4444214 (-) 1059 WP_000360416.1 lipopolysaccharide core heptosyltransferase RfaQ -
  EW646_RS22025 (EW646_22585) waaA 4444627..4445904 (+) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EW646_RS22030 (EW646_22590) coaD 4445912..4446391 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  EW646_RS22035 (EW646_22595) mutM 4446430..4447239 (-) 810 WP_001114545.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EW646_RS22040 (EW646_22600) rpmG 4447337..4447504 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  EW646_RS22045 (EW646_22605) rpmB 4447525..4447761 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  EW646_RS22050 (EW646_22610) radC 4447978..4448646 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EW646_RS22055 (EW646_22615) coaBC 4448818..4450038 (+) 1221 WP_000050121.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EW646_RS22060 (EW646_22620) dut 4450016..4450474 (+) 459 WP_000976070.1 dUTP diphosphatase -
  EW646_RS22065 (EW646_22625) slmA 4450581..4451177 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EW646_RS22070 (EW646_22630) pyrE 4451214..4451855 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EW646_RS22075 (EW646_22635) rph 4451921..4452637 (-) 717 WP_001247093.1 ribonuclease PH -
  EW646_RS22080 (EW646_22640) yicC 4452764..4453627 (+) 864 WP_000621340.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=304839 EW646_RS22050 WP_001297375.1 4447978..4448646(-) (radC) [Escherichia coli O145 strain RM8988-C1]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=304839 EW646_RS22050 WP_001297375.1 4447978..4448646(-) (radC) [Escherichia coli O145 strain RM8988-C1]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment