Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LCHO_RS02765 Genome accession   NC_010524
Coordinates   595435..597159 (-) Length   574 a.a.
NCBI ID   WP_012345587.1    Uniprot ID   B1XYU1
Organism   Leptothrix cholodnii SP-6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 590435..602159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCHO_RS02735 (Lcho_0544) - 590984..591484 (+) 501 WP_012345581.1 NUDIX domain-containing protein -
  LCHO_RS02740 (Lcho_0545) - 591501..591707 (-) 207 WP_012345582.1 DNA gyrase inhibitor YacG -
  LCHO_RS02745 (Lcho_0546) zapD 591740..592495 (-) 756 WP_012345583.1 cell division protein ZapD -
  LCHO_RS02750 (Lcho_0547) coaE 592576..593190 (-) 615 WP_012345584.1 dephospho-CoA kinase -
  LCHO_RS02755 (Lcho_0548) - 593202..594149 (-) 948 WP_012345585.1 prepilin peptidase -
  LCHO_RS02760 (Lcho_0549) pilC 594158..595384 (-) 1227 WP_012345586.1 type II secretion system F family protein Machinery gene
  LCHO_RS02765 (Lcho_0550) pilB 595435..597159 (-) 1725 WP_012345587.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LCHO_RS02770 (Lcho_0551) htpG 597526..599430 (+) 1905 WP_012345588.1 molecular chaperone HtpG -
  LCHO_RS02775 (Lcho_0552) - 599447..600529 (-) 1083 WP_012345589.1 serine/threonine protein kinase -
  LCHO_RS21910 (Lcho_0553) - 600561..601370 (-) 810 WP_012345590.1 DUF2726 domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62702.95 Da        Isoelectric Point: 5.6405

>NTDB_id=30435 LCHO_RS02765 WP_012345587.1 595435..597159(-) (pilB) [Leptothrix cholodnii SP-6]
MAAETLSDAPQSTLSGVARVLVHAGKLNARAAEELARLARDQRRNFVAAAVGANLLSSADLAHTLSHALAVPLLDLDAVD
VSKLPQNLVDARTLSQYQVLVLGKRGNRLFIGGADPTDQEAVDRIKFATQLTPEWVIVEYDKLAKIVEAKSTSATEALES
IVAGDFEFDVEEQDPNAKPDEEVTTDVEDAPVVRFLQKMLIDAINMRASDLHFEPYEQTYRVRFRIDGELREITQPPVAI
KDKLASRIKVISRLDIAEKRVPQDGRMKLKFGNKAIDFRISTLPTLFGEKIVIRILDPSSAKLGVEALGYEREEKDRLLS
AIKRPYGMILVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGINQVNVNDKAGMNFAVALRAFLRQDPDVIM
VGEIRDIETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLLNMGVPPFNIASSIILISAQRLCRRLCENCKAPAEYPREAL
LRAGFKPDEVDGSWKPYKAVGCSACNNGYRGRVGVYQVMPITEAIQRVILAEGTALDIAEQAARDGVRDLRLSGLVKVRA
GATTLEEVISVTNQ

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=30435 LCHO_RS02765 WP_012345587.1 595435..597159(-) (pilB) [Leptothrix cholodnii SP-6]
GTGGCCGCTGAAACACTGTCCGACGCCCCGCAAAGCACCCTCTCCGGGGTGGCCCGCGTGCTGGTGCACGCCGGCAAGCT
GAACGCCCGCGCGGCCGAAGAACTCGCCCGCCTCGCACGTGATCAGCGCCGCAACTTCGTCGCCGCCGCGGTCGGCGCCA
ATCTGCTGAGTTCGGCCGATCTGGCCCACACGCTGTCGCACGCGCTGGCCGTGCCGCTGCTCGACCTCGACGCGGTCGAC
GTCAGCAAGCTGCCGCAGAACCTGGTCGACGCGCGCACGCTGTCGCAGTATCAGGTGCTGGTGCTGGGCAAACGCGGCAA
CCGGCTGTTCATCGGCGGCGCCGACCCGACCGACCAGGAAGCGGTCGACCGCATCAAGTTCGCCACCCAGCTGACGCCCG
AGTGGGTGATCGTCGAATACGACAAGCTGGCCAAGATCGTCGAGGCCAAGAGCACCTCGGCGACCGAGGCGCTCGAATCG
ATCGTCGCCGGCGATTTCGAGTTCGACGTCGAAGAGCAGGATCCGAACGCCAAGCCCGACGAGGAGGTCACCACCGACGT
CGAGGACGCGCCGGTGGTGCGCTTCCTGCAGAAGATGCTGATCGACGCGATCAACATGCGGGCGTCGGACCTGCACTTCG
AGCCCTACGAGCAGACCTACCGGGTGCGCTTTCGCATCGACGGCGAGCTGCGCGAGATCACCCAGCCGCCGGTGGCGATC
AAGGACAAGCTGGCCTCGCGCATCAAGGTCATCAGCCGCCTGGACATCGCCGAAAAACGCGTGCCGCAGGACGGCCGCAT
GAAGCTGAAGTTCGGCAACAAGGCAATCGACTTCCGCATCAGCACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATCCTCGACCCGTCGTCGGCCAAGCTGGGCGTCGAGGCGCTCGGTTACGAGCGCGAGGAGAAGGATCGGCTGCTGTCG
GCCATCAAGCGGCCGTACGGGATGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCTGCCT
GAACATCCTGAACCAGCCCGGCGTCAACATCTCGACCGTCGAGGATCCGGCCGAAATCAACCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGCATGAACTTCGCCGTCGCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATG
GTCGGCGAGATCCGCGACATCGAGACCGCCGACATCGCGATCAAGGCCGCCCAGACCGGCCACCTGGTGCTGTCGACGCT
GCACACCAACGATGCCCCGACCACGCTGACGCGGCTGCTCAACATGGGCGTGCCGCCGTTCAACATCGCCTCGAGCATCA
TCCTGATCTCGGCCCAGCGACTGTGCCGACGCCTGTGCGAGAACTGCAAGGCTCCGGCCGAATACCCGCGCGAGGCGCTG
CTGCGCGCCGGCTTCAAGCCCGACGAGGTCGACGGCTCCTGGAAACCCTACAAGGCGGTCGGCTGCTCGGCCTGCAACAA
CGGCTACCGCGGGCGGGTCGGCGTCTATCAGGTGATGCCGATCACCGAGGCGATCCAGCGCGTCATCCTCGCCGAAGGCA
CCGCGCTCGACATCGCCGAGCAGGCCGCCCGCGACGGCGTGCGCGATCTGCGGCTGTCGGGTCTGGTTAAAGTGCGGGCG
GGCGCCACCACGCTCGAAGAAGTGATTTCCGTGACCAACCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1XYU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.398

98.084

0.514
  pilB Acinetobacter baylyi ADP1

50.702

99.303

0.503
  pilF Neisseria gonorrhoeae MS11

53.346

91.115

0.486
  pilB Legionella pneumophila strain ERS1305867

49.027

98.432

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

44.583

98.084

0.437
  pilB Vibrio cholerae strain A1552

48.084

90.941

0.437
  pilB Vibrio campbellii strain DS40M4

44.643

97.561

0.436

Multiple sequence alignment