Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   DN409_RS22420 Genome accession   NZ_CP031071
Coordinates   4353576..4355897 (-) Length   773 a.a.
NCBI ID   WP_149190929.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN401     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4348576..4360897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN409_RS30985 - 4349700..4349876 (-) 177 WP_001242605.1 hypothetical protein -
  DN409_RS22405 (DN409_22410) - 4350145..4350585 (-) 441 WP_002015302.1 D-tyrosyl-tRNA(Tyr) deacylase -
  DN409_RS22410 (DN409_22415) relA 4350603..4352786 (-) 2184 WP_002015304.1 GTP diphosphokinase -
  DN409_RS22415 (DN409_22420) - 4352998..4353510 (-) 513 WP_002015305.1 adenine phosphoribosyltransferase -
  DN409_RS22420 (DN409_22425) recJ 4353576..4355897 (-) 2322 WP_149190929.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  DN409_RS22425 (DN409_22430) - 4355999..4356892 (-) 894 WP_002034095.1 cation diffusion facilitator family transporter -
  DN409_RS22430 (DN409_22435) secDF 4357190..4359454 (-) 2265 WP_061689581.1 protein translocase subunit SecDF -
  DN409_RS22435 (DN409_22440) - 4359737..4360021 (-) 285 WP_002112000.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 773 a.a.        Molecular weight: 87254.67 Da        Isoelectric Point: 4.9970

>NTDB_id=303225 DN409_RS22420 WP_149190929.1 4353576..4355897(-) (recJ) [Bacillus mycoides strain BPN401]
MLQPKTRWKEKEYNEELVSELANKLQLSPLVTSLFLGRGLNTEDKIVNFLNTADQEFHDPFLLEGMDRTVERVKKAIENG
EQILIFGDYDADGVSSTTVLYLALKELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VQRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFALAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPHKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLNMNDVDELRRRLNEQADVILTAEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGTYAKEISPVAKVSVVGEVSINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITLVYFSEEVLNKFSLEGYKEHIVHASEVAHFDDQYIVLLDLPKGTDELGD
VFKAGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRDHK

Nucleotide


Download         Length: 2322 bp        

>NTDB_id=303225 DN409_RS22420 WP_149190929.1 4353576..4355897(-) (recJ) [Bacillus mycoides strain BPN401]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGAGGAACTAGTGAGTGAATTAGCAAATAAATTGCAACT
ATCACCTCTTGTGACTTCATTATTTCTTGGCAGAGGCTTAAATACAGAAGATAAGATTGTAAACTTTTTAAATACAGCAG
ATCAAGAATTTCATGATCCATTTTTACTTGAAGGAATGGATCGGACTGTAGAACGTGTGAAAAAAGCTATTGAAAATGGA
GAACAAATATTAATATTTGGCGATTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCTCTCAAAGAATT
AGGTGCAGAAGTAGAGTTTTATATTCCAAATCGTTTTACTGAAGGCTATGGGCCAAATGAAGAAGCGTTTCGTTGGGCGC
ACAGTGCAGGGTTCTCACTAATTATTACTGTCGATACTGGTATCGCAGCGGTACATGAAGCGAAGGTAGCGAAGGAACTT
GGAATAGATCTTATTATTACAGATCATCATGAGCCACCACCTGAATTACCAGAGGCACTTGCGATTATTCATCCGAAATT
AGAAGGCGGTGTTTATCCGTTTCATTATTTAGCGGGTGTTGGTGTTGCATTTAAAGTTGCACATGCACTTTTGGGTCGTG
TACCAGAGCATTTGTTGGAAATTGCAGTAATCGGTACGGTAGCCGATTTAGTATCGCTTCATGGTGAAAATAGATTGTTA
GTACAGCGTGGACTGAAGCATATGCGAATGACTCGGAATATCGGTTTAAAGGCGTTATTTAAAGTTGCTAACGTTTCGCA
AAGCGAAATTACAGAAGAAAGCATCGGGTTCGCACTCGCACCTCGTATTAATGCAGTTGGTCGTTTGGAAGATGCAACAC
CTGCTGTACACCTTTTATTATCAGAAGATCCAGAGGAAGCGAAAGAACTAGCTGAAGAAATTGATGAGCTGAACAAACTT
CGAAAAGATATTGTAAAGCAAATTACTGAAGAAGCTATCGCTGAAGTCGAAAATAATTTCCCACCAGAAGAAAATAAAGT
ATTAGTCCTTGCAAAAGAAGGTTGGAATCCAGGGGTAATTGGAATTGTCGCTTCTAAGTTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTAAGCATTGATCCTCATAAAGAAACAGCAAAGGGATCGGCTCGTAGTATTGCAGGATTTGATTTGTTT
GCAAACCTTTCAGATTGCCGTGAATTGTTACCGCATTTCGGAGGTCATCCGATGGCAGCGGGAATGACGCTTAATATGAA
TGATGTGGATGAATTAAGACGCAGGTTAAATGAGCAAGCAGATGTAATTTTAACAGCAGAAGATTTTATTCCGATTACAG
CAGTGGATGCATTTTGTAAAGTAGAGGATGTAACGCTTGCCGCGATTGAGGATATGCAAAAGTTAGCGCCATTTGGCGTA
GGAAATCCGAAACCGCGTATTGCGGTGAAAGATGCCGAATTAGAAAGTATCCGTGCAATTGGATCTGATGGTTCGCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGACACGATTGGATTTGGATTTGGTACTTATGCGAAAGAAATCTCTC
CAGTTGCGAAGGTTTCTGTAGTAGGAGAAGTGTCCATTAATGAATGGAATAACTTTAAAAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGACTGGCGTAGTATGCGTAATGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACATTGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAAGGCTATAAAGAGCATATCGTGC
ATGCATCAGAGGTAGCTCATTTTGATGATCAATACATTGTGCTACTTGATTTGCCGAAAGGAACAGATGAATTAGGTGAT
GTATTTAAGGCTGGATTCCCAGCTCGAATTTACACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACGGTCCCAAC
GAGGGAACATTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGGCAATATGGGGAACAACTGT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAGAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTGATCATAAAT
AA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHHA1 domain (DHHA1)

(315-439)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.713

100

0.528

Multiple sequence alignment