Detailed information NTG0302852

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DT536_RS12550
• Genome accession: NZ_CP031011
• Coordinates: 2581180..2582373 (+)
• Length: 397 a.a.
• NCBI ID: WP_058869561.1
• Uniprot ID: -
• Organism: Acinetobacter johnsonii strain LXL_C1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2576180..2587373

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DT536_RS12540 (DT536_12540)	-	2577302..2579401 (+)	2100	WP_058869560.1	EAL domain-containing protein	-
DT536_RS17010	-	2579788..2579934 (-)	147	WP_171254088.1	hypothetical protein	-
DT536_RS12545 (DT536_12545)	-	2579880..2580941 (+)	1062	WP_010326643.1	3-deoxy-7-phosphoheptulonate synthase	-
DT536_RS12550 (DT536_12550)	pilU	2581180..2582373 (+)	1194	WP_058869561.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DT536_RS12555 (DT536_12555)	-	2582421..2583599 (-)	1179	WP_058869562.1	MFS transporter	-
DT536_RS12560 (DT536_12560)	gcvH	2583859..2584233 (-)	375	WP_004693240.1	glycine cleavage system protein GcvH	-
DT536_RS12565 (DT536_12565)	-	2584305..2585333 (-)	1029	WP_004979806.1	AraC family transcriptional regulator	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44662.05 Da
• Isoelectric Point: 6.5063

>NTDB_id=302852 DT536_RS12550 WP_058869561.1 2581180..2582373(+) (pilU) [Acinetobacter johnsonii strain LXL_C1]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAISVPNVSRFRINVFQQQLHVGMVIRTITAEIPNFTKLQLPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANPQLSSNSALSFDQAVAEKAKDAEEEKADA

Nucleotide

• Length: 1194 bp

>NTDB_id=302852 DT536_RS12550 WP_058869561.1 2581180..2582373(+) (pilU) [Acinetobacter johnsonii strain LXL_C1]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGCTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTTATGAAGCCATTGGGTCAACAAAATTTGC
CATCTGATCAGACTAAGCTCTTTGCTTATAGCTTAATGAACGAAAAGCAACGATTGGAGTTCGAAACTGAGCTTGAGTGT
AATTTTGCAATTAGCGTTCCCAATGTTTCGCGTTTTCGTATCAATGTTTTTCAGCAGCAACTGCATGTGGGGATGGTGAT
TCGTACGATTACCGCGGAAATTCCTAACTTCACCAAGTTACAACTTCCAACGTCTTTAAAAGACGTGATTATGGAAAAAC
GTGGCTTAGTATTGGTAGTTGGGGGTACAGGGTCGGGTAAATCTACGTCTTTGGCGGCCATGATTGATCATCGAAATGAA
AACTCGGCAGGGCACATTATTACGGTTGAAGACCCAGTGGAATATGTTCACAAGCATAAGAAATCGATGATTACTCATCG
GGAAGTGGGTGTAGACTGTCATTCTTGGCACAATGCTTTGAAAAATACTTTGCGCCAAGCGCCCGATGTCATTTTAATTG
GTGAAATTCGTGACACGGAGACTATGGAGCATGCCATAGCTTTTGCAGAAACAGGCCACTTATGCCTAGGCACATTACAT
GCTAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTCCCAGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCCAATATGAAGGCGATTATTTCACAGCGTCTGGTGCGAACTGAAGATGGTCGTGGTCGCCGAGCAGCAGTGGAAA
TCATGCTCAATACGCCGTTGATGTCAGACTTGATACTTAAAGGGAATTTCCATGAACTCAAAGAAGTGATGTCGAAGTCA
CGAGAGCTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATTGCTTACGAAGAAGCGCT
ACGAAATGCAGACTCGGTCAATGAATTACGTCTACAGATTAAATTAAAGAGTAGTCGTGCCAACCCGCAGTTAAGCTCAA
ATAGTGCTTTGTCTTTTGATCAAGCGGTTGCTGAAAAAGCCAAAGACGCTGAAGAAGAGAAGGCCGACGCCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (122-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.053	90.428	0.534
pilU	Acinetobacter baylyi ADP1	54.545	91.436	0.499
pilU	Vibrio cholerae strain A1552	50.289	87.154	0.438
pilT	Legionella pneumophila strain Lp02	42.183	85.39	0.36
pilT	Legionella pneumophila strain ERS1305867	42.183	85.39	0.36