Detailed information NTG0301325

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DPR03_RS06740
• Genome accession: NZ_CP030754
• Coordinates: 1394782..1395960 (+)
• Length: 392 a.a.
• NCBI ID: WP_071320716.1
• Uniprot ID: -
• Organism: Acinetobacter schindleri strain H3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1389782..1400960

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DPR03_RS06725	-	1391117..1392229 (+)	1113	WP_071320714.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
DPR03_RS06730	-	1392435..1392965 (+)	531	WP_005222291.1	hypothetical protein	-
DPR03_RS06735	-	1393487..1394536 (+)	1050	WP_071320715.1	3-deoxy-7-phosphoheptulonate synthase	-
DPR03_RS06740	pilU	1394782..1395960 (+)	1179	WP_071320716.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DPR03_RS06745	pilU	1396371..1397576 (+)	1206	WP_071320717.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DPR03_RS06750	-	1397636..1398814 (-)	1179	WP_071320718.1	MFS transporter	-
DPR03_RS06755	gcvH	1399046..1399420 (-)	375	WP_048881090.1	glycine cleavage system protein GcvH	-
DPR03_RS06760	-	1399493..1400521 (-)	1029	WP_071320719.1	AraC family transcriptional regulator	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 44504.13 Da
• Isoelectric Point: 6.8329

>NTDB_id=301325 DPR03_RS06740 WP_071320716.1 1394782..1395960(+) (pilU) [Acinetobacter schindleri strain H3]
MYTPELLDEARKFMHHMLTKVVEYGGSDLFITADFPPSIKQQGLMKPFGQQKLAANKTRLFAYALMNERQIKEFESVMEC
NFGISVPGVSRFRVNVFQQQHNVGMVIRTITTEIPSFQQLGLPNSLQQVIMEKRGLVLIVGATGVGKSTTLAAMIDHRNT
YSEGHIITIEDPVEYVYQHKKSMITQREIGVDCVSWHNALINTMRQAPDVIVIGEIRDTESMEHAIAFAESGHLCLATLH
SNNADQALDRIINFFPEERRNQLLMDLSANMKAVISQRLIRTEDGQGRRAAVEILLNTPLVSDFIRKGDFYELKTIMARS
RELGMQTFDQALFDLYNQGVISYDEALRNADSSNEMRLQIKLKSSRINPALQEEGSKMRILEQSKETTLSRI

Nucleotide

• Length: 1179 bp

>NTDB_id=301325 DPR03_RS06740 WP_071320716.1 1394782..1395960(+) (pilU) [Acinetobacter schindleri strain H3]
ATGTATACACCCGAATTGCTCGATGAAGCACGCAAATTTATGCACCATATGCTGACCAAAGTGGTTGAATATGGAGGTTC
CGATCTTTTTATTACCGCAGATTTTCCACCAAGTATCAAACAGCAGGGTCTGATGAAGCCTTTTGGTCAGCAAAAACTGG
CAGCGAATAAAACCCGTTTGTTTGCCTATGCTTTAATGAATGAACGACAGATCAAGGAATTTGAATCAGTGATGGAATGC
AATTTCGGTATCAGTGTACCGGGTGTATCACGCTTCCGGGTCAATGTCTTTCAGCAACAGCATAATGTTGGGATGGTAAT
CAGAACCATTACTACGGAGATTCCAAGCTTTCAGCAATTAGGACTACCAAACTCTTTGCAACAAGTGATTATGGAAAAAC
GTGGGCTGGTTCTGATTGTAGGTGCGACAGGTGTTGGTAAATCTACCACACTTGCAGCCATGATTGATCACCGCAATACC
TATTCTGAAGGACATATTATTACCATAGAAGATCCTGTGGAATACGTGTATCAACATAAAAAGTCCATGATTACCCAACG
TGAAATTGGCGTAGATTGTGTTTCCTGGCATAACGCGCTGATCAATACCATGCGTCAGGCACCGGATGTGATCGTGATTG
GTGAAATCCGGGATACCGAAAGTATGGAGCATGCAATTGCCTTTGCTGAATCCGGTCATCTGTGTTTGGCCACCCTGCAT
TCTAATAATGCCGATCAGGCTTTAGACCGAATTATTAATTTCTTCCCGGAAGAACGCCGCAATCAGCTATTAATGGATTT
ATCCGCCAATATGAAAGCTGTGATTTCGCAACGTTTGATCCGTACCGAGGATGGGCAAGGCCGTAGAGCTGCCGTTGAAA
TTTTATTGAATACGCCACTGGTGTCTGATTTTATCCGTAAAGGAGATTTTTACGAGTTGAAAACTATTATGGCGCGTTCG
CGTGAACTCGGCATGCAGACCTTTGATCAGGCATTATTTGATTTGTATAACCAGGGAGTTATTTCTTATGACGAAGCCTT
GCGTAATGCAGATTCAAGTAATGAAATGCGTTTGCAGATCAAATTAAAGAGCAGTCGCATCAATCCCGCTCTTCAGGAAG
AAGGAAGTAAAATGAGGATACTGGAACAGTCTAAGGAAACTACACTTTCCAGAATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (64-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.333	88.776	0.518
pilU	Acinetobacter baylyi ADP1	55.085	90.306	0.497
pilU	Vibrio cholerae strain A1552	49.577	90.561	0.449
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	86.48	0.372