Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DPR03_RS06745 Genome accession   NZ_CP030754
Coordinates   1396371..1397576 (+) Length   401 a.a.
NCBI ID   WP_071320717.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain H3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1391371..1402576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPR03_RS06730 - 1392435..1392965 (+) 531 WP_005222291.1 hypothetical protein -
  DPR03_RS06735 - 1393487..1394536 (+) 1050 WP_071320715.1 3-deoxy-7-phosphoheptulonate synthase -
  DPR03_RS06740 pilU 1394782..1395960 (+) 1179 WP_071320716.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DPR03_RS06745 pilU 1396371..1397576 (+) 1206 WP_071320717.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DPR03_RS06750 - 1397636..1398814 (-) 1179 WP_071320718.1 MFS transporter -
  DPR03_RS06755 gcvH 1399046..1399420 (-) 375 WP_048881090.1 glycine cleavage system protein GcvH -
  DPR03_RS06760 - 1399493..1400521 (-) 1029 WP_071320719.1 AraC family transcriptional regulator -
  DPR03_RS06765 - 1400674..1401123 (-) 450 WP_071320720.1 MaoC family dehydratase -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 45237.60 Da        Isoelectric Point: 6.3946

>NTDB_id=301326 DPR03_RS06745 WP_071320717.1 1396371..1397576(+) (pilU) [Acinetobacter schindleri strain H3]
MFTAELLEEARKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTPEKTKLFAYALMNEKQRKEYEAELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIVSQRLVRTEDGKGRRAAVEIMLNTPLVSDLILKGDFHELKAIMGKS
RELGMQTFDQALFDLYNEGAISYEEALRNADSTNELRLQIKLKSTRANPAMNNTATAFSMVEEKKPDNETDEDTSTTEQS
S

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=301326 DPR03_RS06745 WP_071320717.1 1396371..1397576(+) (pilU) [Acinetobacter schindleri strain H3]
ATGTTTACGGCAGAGTTATTAGAAGAAGCACGTAAGTTTATGCATCATATGCTGACCAAGGTCGTTGAATATGGTGGTTC
AGACTTGTTCATTACCGCAGACTTTCCTCCGAGTATCAAACATCAGGGATTAATGAAACCTTTTGGGCAACAGGAACTCA
CCCCAGAAAAAACCAAACTGTTTGCCTATGCACTGATGAACGAAAAGCAGCGTAAAGAATATGAAGCAGAACTAGAATGT
AACTTTGCCATTACTGTACCGGGCGTTTCACGTTTCCGTGTCAACGTGTTCCAGCAGCAGCTCAATGTCGGCATGGTGAT
CCGGACCATCACTGCAGAAATTCCGAATTTCCAGAAGTTAAAATTACCAGAGTCACTCAAGCATGTGATTATGGAAAAGC
GCGGTCTGGTACTCGTCGTGGGCGGTACAGGTTCAGGTAAGTCGACTTCGCTTGCTGCGATGATTGATTATCGTAATGAA
AACTCAGCAGGCCATATCATTACCGTAGAAGACCCGGTGGAATATGTACATAAACATAAGAAGTCAATGATCACCCATCG
CGAGGTCGGTGTAGATAGCCATTCCTGGCATAATGCCTTAAAAAATACCTTGCGTCAGGCACCCGATGTGATTCTGATTG
GTGAAATCCGGGATACGGAAACCATGGAACATGCAATTGCCTTTGCCGAAACAGGGCATCTCTGTCTGGGGACGCTGCAT
TCCAACAATGCCAACCAGACCCTCGACCGGATTATTAACTTCTTCCCGGAAGAACGCCGTAATCAGTTATTGATGGATCT
GTCTTCCAATATGAAAGCGATTGTCTCTCAGCGTTTGGTCCGTACTGAAGATGGCAAGGGGCGTCGTGCCGCGGTCGAAA
TTATGCTGAATACACCATTGGTATCAGATCTTATTCTGAAAGGTGATTTCCATGAATTAAAAGCAATTATGGGTAAATCA
CGTGAACTTGGTATGCAGACCTTTGATCAGGCATTATTTGATCTTTATAATGAAGGTGCGATTTCTTATGAAGAAGCATT
GCGTAATGCTGATTCGACCAATGAGCTTCGTCTGCAAATCAAACTGAAAAGTACGCGTGCTAATCCAGCCATGAATAATA
CGGCCACCGCATTTAGCATGGTGGAAGAGAAAAAGCCGGACAATGAAACGGATGAAGATACCAGTACCACAGAACAAAGT
TCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.207

86.783

0.531
  pilU Acinetobacter baylyi ADP1

54.19

89.277

0.484
  pilU Vibrio cholerae strain A1552

50.986

88.529

0.451

Multiple sequence alignment