Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DM558_RS02250 Genome accession   NZ_CP029822
Coordinates   456904..459162 (+) Length   752 a.a.
NCBI ID   WP_127161863.1    Uniprot ID   A0A3Q9JHF4
Organism   Entomomonas moraniae strain QZS01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 430564..463341 456904..459162 within 0


Gene organization within MGE regions


Location: 430564..463341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM558_RS02160 (DM558_02165) - 430564..431061 (+) 498 WP_127161849.1 hypothetical protein -
  DM558_RS02165 (DM558_02170) - 431117..431548 (-) 432 WP_127161850.1 hypothetical protein -
  DM558_RS02170 (DM558_02175) - 431984..435829 (+) 3846 WP_127161851.1 SIR2 family protein -
  DM558_RS02175 (DM558_02180) - 436370..437269 (+) 900 WP_177412499.1 phage capsid protein -
  DM558_RS02180 (DM558_02185) - 437335..437913 (+) 579 WP_127161852.1 hypothetical protein -
  DM558_RS15575 - 437906..440047 (+) 2142 WP_164731222.1 hypothetical protein -
  DM558_RS02190 (DM558_02200) - 440040..440492 (+) 453 WP_127161853.1 GNAT family N-acetyltransferase -
  DM558_RS02195 (DM558_02205) - 440495..441100 (+) 606 WP_127161854.1 hypothetical protein -
  DM558_RS02200 (DM558_02210) - 441102..443105 (+) 2004 WP_127161855.1 hypothetical protein -
  DM558_RS02205 (DM558_02215) - 443098..447330 (+) 4233 WP_127161856.1 hypothetical protein -
  DM558_RS02210 (DM558_02220) - 447346..447678 (+) 333 WP_127161857.1 hypothetical protein -
  DM558_RS02215 (DM558_02225) terL 447950..449560 (+) 1611 WP_127161858.1 phage terminase large subunit -
  DM558_RS02220 (DM558_02230) - 449910..451496 (+) 1587 WP_127161859.1 hypothetical protein -
  DM558_RS02225 (DM558_02235) - 451551..453938 (-) 2388 WP_127164787.1 penicillin-binding protein 1A -
  DM558_RS02230 (DM558_02240) pilM 454160..455191 (+) 1032 WP_127164788.1 type IV pilus assembly protein PilM -
  DM558_RS02235 (DM558_02245) - 455191..455760 (+) 570 WP_127161860.1 PilN domain-containing protein -
  DM558_RS02240 (DM558_02250) - 455757..456359 (+) 603 WP_127161861.1 type 4a pilus biogenesis protein PilO -
  DM558_RS02245 (DM558_02255) - 456362..456886 (+) 525 WP_164731223.1 pilus assembly protein PilP -
  DM558_RS02250 (DM558_02260) pilQ 456904..459162 (+) 2259 WP_127161863.1 type IV pilus secretin PilQ Machinery gene
  DM558_RS02255 (DM558_02265) aroK 459167..459679 (+) 513 WP_127161864.1 shikimate kinase AroK -
  DM558_RS02260 (DM558_02270) aroB 459713..460807 (+) 1095 WP_127161865.1 3-dehydroquinate synthase -
  DM558_RS02265 (DM558_02275) - 460820..462508 (+) 1689 WP_127161866.1 AAA family ATPase -
  DM558_RS02270 (DM558_02280) queF 462511..463341 (+) 831 WP_127164789.1 NADPH-dependent 7-cyano-7-deazaguanine reductase QueF -

Sequence

Protein


Download         Length: 752 a.a.        Molecular weight: 81947.07 Da        Isoelectric Point: 9.0204

>NTDB_id=296195 DM558_RS02250 WP_127161863.1 456904..459162(+) (pilQ) [Entomomonas moraniae strain QZS01]
MKNNTLIKHIEKSQSARFSRLAMALLSMAVTPMLFAANLKDMEVASLPGEQVQLKLIFDEPVKLPSGYTIDQPARIALDL
PGVTNQVKEKRRTVDIGNVKNLTVVEAGNRTRLVVGLNSLVPYTSRVEGNNVYILLGDSNTNTATKPATLTVATSSVKPT
TSTVKTNTTSIVPSISGVDKIDSVDFRRGDGGEGDIIIQLSNSNISPEIQERNGRIVLSFPKTQLPEALRVRLDVTDFAT
PVKFVNSTSDANGSTITIEPRGNYDYLVYQANDKLTVSVKSISAEDKQRHLEDQGIFKGERLSLNFQNIEVRSVLQILAD
FTKLNLVASDTVTGNITLRLQNVPWDQALDIIMRTRGLAKRQEGNILIIAPADELAESARRIYDAQETLKATQPLRRELI
QVNYADATAIIGLFQATMSQTNSIQTGQTTSGAGTYSANMTMNDRGSLTVDKRTNTILAYQTEERLQELRRIVAQLDKPV
RQVMIETRIVEANVSFAKSLGVRWAADIGKTGNLHSYGGADGRYNSKSGSDGNFANTGTNIGFDEDKNAYTIPAAAPVVD
LGAANATSGFGIGYITKNLMLDLKLNAMQKSSQGEIISQPKVTTSDKEKATIMKGQEVPYQEATSSGATSTSFKKAVLSL
EVTPQITPDNQVMMEVKVNKDSVDYSRSVAGVPPIDTNEVTAKVLVGDGETIVLGGVFQNTQSKTVEKVPYLGDLPVMGR
LFRRDTVDNNKQELLIFITPRIIDNKAFAVSR

Nucleotide


Download         Length: 2259 bp        

>NTDB_id=296195 DM558_RS02250 WP_127161863.1 456904..459162(+) (pilQ) [Entomomonas moraniae strain QZS01]
ATGAAAAATAATACCCTAATTAAGCACATTGAAAAATCACAAAGTGCTCGTTTTAGTCGATTAGCTATGGCTTTATTAAG
TATGGCTGTTACTCCAATGCTTTTTGCTGCAAACTTAAAGGATATGGAAGTAGCTTCTTTACCGGGGGAGCAAGTCCAGC
TTAAGCTAATCTTTGATGAACCCGTAAAATTGCCATCAGGTTATACGATTGATCAACCTGCTCGTATCGCTCTGGATTTG
CCTGGTGTGACAAACCAAGTTAAAGAAAAAAGACGAACAGTAGATATTGGAAATGTTAAAAATCTAACCGTGGTAGAGGC
AGGTAATAGAACTCGCCTTGTCGTTGGGCTAAACTCCTTAGTTCCTTATACTTCTCGCGTAGAAGGTAATAATGTTTATA
TTTTATTGGGCGATAGCAACACTAATACAGCTACAAAGCCAGCTACTTTGACAGTAGCAACATCAAGCGTCAAACCAACT
ACTAGTACAGTAAAAACAAATACGACATCTATTGTGCCTAGTATTAGTGGTGTAGATAAAATAGACAGTGTTGATTTTAG
ACGTGGTGATGGTGGTGAAGGCGATATTATAATTCAGTTATCTAATAGTAACATCAGCCCTGAGATTCAAGAGCGTAATG
GTCGCATTGTACTCAGCTTTCCTAAAACTCAGTTACCTGAGGCTCTCCGCGTACGTTTGGATGTGACAGATTTTGCTACA
CCTGTTAAATTTGTTAACTCAACATCTGATGCAAATGGCTCAACGATTACTATTGAACCAAGAGGGAATTACGATTACTT
AGTCTATCAAGCCAATGATAAATTAACGGTAAGTGTTAAAAGTATTTCTGCGGAAGATAAACAGCGCCATTTAGAAGACC
AAGGTATTTTTAAAGGTGAGCGTTTGTCTTTAAACTTCCAAAATATTGAAGTGCGTAGTGTGTTACAAATTTTAGCGGAT
TTTACTAAACTAAACTTAGTTGCGAGTGATACTGTTACAGGTAATATTACGCTACGGTTACAAAATGTTCCTTGGGATCA
AGCATTAGATATTATTATGCGTACCCGTGGCCTTGCTAAACGTCAAGAAGGTAATATTCTAATTATTGCTCCAGCGGATG
AGTTGGCGGAGTCTGCACGCCGTATTTACGATGCACAAGAAACGCTAAAGGCAACGCAACCACTTCGTCGTGAGCTTATT
CAAGTGAATTATGCTGATGCGACGGCTATTATTGGTTTGTTCCAAGCCACGATGTCACAAACAAATAGTATTCAAACAGG
GCAGACAACATCAGGTGCCGGTACTTACTCAGCGAATATGACTATGAATGACCGTGGATCACTCACCGTTGATAAAAGAA
CCAATACTATTCTAGCTTATCAAACGGAAGAGCGGCTACAAGAGCTAAGACGTATCGTTGCCCAGTTGGATAAACCCGTG
CGTCAAGTTATGATCGAAACGCGTATTGTTGAAGCTAATGTATCTTTTGCTAAAAGCCTAGGAGTTCGTTGGGCCGCCGA
TATTGGTAAAACAGGTAACCTTCATTCTTATGGTGGTGCGGATGGACGTTATAATAGTAAAAGTGGTAGTGATGGTAACT
TTGCTAATACAGGTACTAATATTGGTTTTGACGAAGATAAAAATGCGTATACAATTCCAGCAGCAGCACCTGTTGTTGAC
TTAGGTGCGGCAAATGCTACTTCAGGTTTTGGTATTGGCTATATTACAAAAAACCTAATGCTGGATCTTAAACTTAATGC
GATGCAGAAATCGAGCCAAGGAGAGATTATCTCCCAGCCTAAGGTGACTACATCTGATAAAGAAAAAGCCACAATTATGA
AAGGGCAAGAGGTGCCTTATCAAGAAGCAACTTCAAGTGGTGCTACATCTACTTCTTTCAAAAAAGCTGTACTATCTCTT
GAAGTAACACCGCAAATTACACCCGATAATCAGGTGATGATGGAAGTTAAAGTAAATAAAGACTCTGTCGACTATTCAAG
GTCAGTAGCTGGTGTTCCACCTATTGATACTAACGAAGTAACAGCAAAAGTTTTAGTAGGTGATGGGGAAACAATAGTCT
TAGGTGGTGTTTTTCAAAATACACAATCTAAAACGGTTGAAAAAGTACCTTATCTTGGAGATCTTCCTGTGATGGGGCGC
TTATTCCGACGTGATACAGTGGATAATAATAAACAAGAGTTGTTAATTTTTATTACTCCTCGTATTATTGATAATAAGGC
ATTTGCTGTATCGAGATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(62-128)

Secretin and TonB N terminus short domain (STN)

(325-371)

Bacterial type II/III secretion system short domain (Secretin_N)

(399-482)

Bacterial type II and III secretion system protein (Secretin)

(587-744)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9JHF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

56.581

98.005

0.555
  pilQ Acinetobacter baumannii strain A118

36.807

100

0.371
  pilQ Acinetobacter baumannii D1279779

36.807

100

0.371
  comQ Acinetobacter baylyi ADP1

38.515

94.947

0.366

Multiple sequence alignment