Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SBAL195_RS16420 Genome accession   NC_009997
Coordinates   3752938..3754050 (-) Length   370 a.a.
NCBI ID   WP_006085705.1    Uniprot ID   -
Organism   Shewanella baltica OS195     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3747938..3759050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBAL195_RS16385 (Sbal195_3174) - 3748425..3748985 (-) 561 WP_006082509.1 DUF4136 domain-containing protein -
  SBAL195_RS16390 (Sbal195_3175) - 3749166..3749483 (+) 318 WP_006085701.1 hypothetical protein -
  SBAL195_RS16395 (Sbal195_3176) yciH 3749662..3749991 (+) 330 WP_037391075.1 stress response translation initiation inhibitor YciH -
  SBAL195_RS16400 (Sbal195_3177) - 3750058..3750621 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  SBAL195_RS16405 (Sbal195_3178) ruvX 3750651..3751076 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  SBAL195_RS16410 (Sbal195_3179) hemH 3751165..3752178 (-) 1014 WP_006085703.1 ferrochelatase -
  SBAL195_RS16415 (Sbal195_3180) - 3752211..3752750 (-) 540 WP_012197407.1 glutathione peroxidase -
  SBAL195_RS16420 (Sbal195_3181) pilU 3752938..3754050 (-) 1113 WP_006085705.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SBAL195_RS16425 (Sbal195_3182) pilT 3754060..3755097 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  SBAL195_RS16430 (Sbal195_3183) - 3755133..3755831 (+) 699 WP_012197408.1 YggS family pyridoxal phosphate-dependent enzyme -
  SBAL195_RS16435 (Sbal195_3184) proC 3756047..3756865 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  SBAL195_RS16440 (Sbal195_3185) - 3756914..3757462 (+) 549 WP_006082520.1 YggT family protein -
  SBAL195_RS16445 (Sbal195_3186) yggU 3757462..3757761 (+) 300 WP_006082521.1 DUF167 family protein YggU -
  SBAL195_RS16450 (Sbal195_3187) - 3757893..3758327 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40986.13 Da        Isoelectric Point: 6.2352

>NTDB_id=29449 SBAL195_RS16420 WP_006085705.1 3752938..3754050(-) (pilU) [Shewanella baltica OS195]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=29449 SBAL195_RS16420 WP_006085705.1 3752938..3754050(-) (pilU) [Shewanella baltica OS195]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCTTCCGACTTGTTTATCACAGCTGGCTTTCCGCCAAG
TGCTAAGATTGACGGTGAGTTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCACTGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCGACTCGTGAGTGTAACTTTGCTTTTGCGGCAAAGGATTTAGGTCGT
TTTCGGGTGAGTGCATTTTGGCAGCGTGAATCGCCTGGCTGCGTGATGCGCCGTATCGAAACTAAAATCCCTGATGTTGA
TGATTTAAAACTGCCACCCATTCTGAAAGATCTGGTGATGAGTAAACGTGGCCTCATCATTATGGTGGGCGGAACGGGTA
CGGGTAAGTCAACATCTCTGGCGGCCTTGGTGGGATATCGTAATGGTCATGCTCGTGGTCATATTTTGACTATCGAAGAC
CCAGTCGAATTCGTTCACGACCACAGAAAAAGCATTATCACCCAGCGTGAAGTGGGCATAGATACTGAATCTTTCGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCACCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CCCTGTCGTTTGCGGAAACAGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAACAGTTATTATTCGATTTGTCATTGAATTTACGCGGGATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGCACTGGGCGTCGCGCCGCGATTGAAGTATTGATCAACACCCCAAGGGTAGCGAGTTTAA
TTGCGAAGAATGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAGGGCATGCAGACGTTCGATCAAGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCTAACGATTTACGTTT
GATGATTAAGTTGCAGAATAAAGAATCCACGGGCTCAGGTTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment