Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   DJ533_RS03775 Genome accession   NZ_CP029397
Coordinates   298388..299170 (+) Length   260 a.a.
NCBI ID   WP_065993309.1    Uniprot ID   A0A2S2F9Y7
Organism   Acinetobacter defluvii strain WCHA30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 293388..304170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ533_RS03760 (DJ533_03770) ponA 293959..296538 (-) 2580 WP_065993306.1 penicillin-binding protein PBP1a -
  DJ533_RS03765 (DJ533_03775) comM 296701..297759 (+) 1059 WP_065993307.1 pilus assembly protein PilM Machinery gene
  DJ533_RS03770 (DJ533_03780) pilN 297759..298391 (+) 633 WP_065993308.1 PilN domain-containing protein Machinery gene
  DJ533_RS03775 (DJ533_03785) pilO 298388..299170 (+) 783 WP_065993309.1 type 4a pilus biogenesis protein PilO Machinery gene
  DJ533_RS03780 (DJ533_03790) pilP 299173..299700 (+) 528 WP_065993310.1 pilus assembly protein PilP Machinery gene
  DJ533_RS03785 (DJ533_03795) pilQ 299766..301885 (+) 2120 Protein_241 type IV pilus secretin PilQ -
  DJ533_RS03790 (DJ533_03800) aroK 301922..302464 (+) 543 WP_065993311.1 shikimate kinase AroK -
  DJ533_RS03795 (DJ533_03805) aroB 302490..303569 (+) 1080 WP_065993312.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 260 a.a.        Molecular weight: 29162.04 Da        Isoelectric Point: 5.4276

>NTDB_id=292212 DJ533_RS03775 WP_065993309.1 298388..299170(+) (pilO) [Acinetobacter defluvii strain WCHA30]
MSRLDLEKADSVKDAPQKKKMTVDKFFQQFNTLDMNNYGSWPMSVKITCWLFILFVVGAIGYFLVMKPKLDAIDSARAQE
QTLLNEFKEKDSKLRNLQQYQAQLQQMQMDFNQQLEQLPKETEIPGLVEDINVTGVSSGLKFKNIKLENEVQQEFFIEQP
ISIEATGDYHSFGGFVSGIAALPRIVTLHDFTITAEAPKDAKSDMPLVNYTLNAKTYRYIGTDVETATTENVPQSTNAAT
TGTASSSNNSSQTTSKKGRK

Nucleotide


Download         Length: 783 bp        

>NTDB_id=292212 DJ533_RS03775 WP_065993309.1 298388..299170(+) (pilO) [Acinetobacter defluvii strain WCHA30]
ATGAGTCGTTTAGATCTTGAAAAAGCAGATTCAGTCAAAGATGCACCACAAAAGAAAAAAATGACGGTAGATAAATTCTT
CCAGCAATTTAATACCTTAGATATGAATAACTATGGTAGTTGGCCAATGTCGGTCAAGATTACCTGTTGGTTATTTATCT
TATTCGTGGTTGGTGCGATCGGTTATTTTCTTGTGATGAAACCGAAGCTAGATGCGATAGATTCGGCCCGTGCACAAGAG
CAAACGTTGTTAAATGAGTTTAAAGAAAAAGATTCGAAACTTAGAAATCTTCAGCAATATCAAGCACAGTTACAACAAAT
GCAAATGGACTTTAATCAACAATTGGAACAGCTACCCAAAGAAACTGAAATTCCAGGTTTGGTTGAAGACATTAACGTGA
CTGGCGTAAGCTCTGGTTTGAAATTTAAAAATATCAAACTAGAAAACGAAGTGCAGCAAGAATTCTTTATTGAGCAACCA
ATTTCAATAGAAGCAACAGGGGATTATCATTCATTTGGTGGTTTTGTGAGTGGCATTGCTGCTTTACCACGAATTGTTAC
CTTGCATGATTTTACCATTACAGCTGAAGCACCAAAGGATGCCAAATCTGATATGCCTTTAGTAAATTATACTTTAAATG
CAAAAACCTATCGTTATATTGGTACAGATGTTGAAACAGCAACAACAGAGAATGTTCCTCAAAGTACAAATGCAGCAACG
ACAGGTACAGCCTCAAGCTCAAATAACTCTTCACAAACAACAAGTAAAAAGGGGAGAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2F9Y7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

65.748

97.692

0.642
  comO Acinetobacter baylyi ADP1

71.622

85.385

0.612

Multiple sequence alignment