Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   DIW83_RS01505 Genome accession   NZ_CP029351
Coordinates   314079..314819 (+) Length   246 a.a.
NCBI ID   WP_016803752.1    Uniprot ID   A0A2L1VMD3
Organism   Acinetobacter nosocomialis strain NCTC 8102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 309079..319819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DIW83_RS01490 (DIW83_01490) ponA 309660..312218 (-) 2559 WP_016803753.1 penicillin-binding protein PBP1a -
  DIW83_RS01495 (DIW83_01495) comM 312380..313438 (+) 1059 WP_004711654.1 pilus assembly protein PilM Machinery gene
  DIW83_RS01500 (DIW83_01500) pilN 313438..314082 (+) 645 WP_006581060.1 PilN domain-containing protein Machinery gene
  DIW83_RS01505 (DIW83_01505) pilO 314079..314819 (+) 741 WP_016803752.1 type 4a pilus biogenesis protein PilO Machinery gene
  DIW83_RS01510 (DIW83_01510) pilP 314830..315357 (+) 528 WP_006582542.1 pilus assembly protein PilP Machinery gene
  DIW83_RS01515 (DIW83_01515) pilQ 315420..317585 (+) 2166 WP_016803751.1 type IV pilus secretin PilQ family protein Machinery gene
  DIW83_RS01520 (DIW83_01520) aroK 317597..318166 (+) 570 WP_002051689.1 shikimate kinase AroK -
  DIW83_RS01525 (DIW83_01525) aroB 318195..319277 (+) 1083 WP_016803750.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27639.43 Da        Isoelectric Point: 4.9673

>NTDB_id=291881 DIW83_RS01505 WP_016803752.1 314079..314819(+) (pilO) [Acinetobacter nosocomialis strain NCTC 8102]
MSQDELQELSLEQSASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
GNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSISALPRIVTMHDFVVEAAQAKDSKSDIPVLNYSIKAKTYRYIGVTEASAQPAKAAAATAPTS
PNAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=291881 DIW83_RS01505 WP_016803752.1 314079..314819(+) (pilO) [Acinetobacter nosocomialis strain NCTC 8102]
ATGAGTCAAGATGAACTGCAAGAATTATCATTAGAGCAATCAGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTTGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGTTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCCCAAGCTCAGGAA
GGTAATTTATTAAATGAATTTCGAGAAAAAGACTCGAAATTACGTAATCTTCAGCAGTATCAAGTGCAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAATTAGAACAATTGCCGAAAGAAACAGAGATTCCAAGTTTAGTTGAGGATATTAACTTAA
CTGGTGTGAACTCTGGATTGAAATTTAAAAATATTCGTTTAGAGGATGAAGTAAAGCAGGAAATTTTTATTGAACAACCA
ATTGCCATCGAAGCAACAGGCGACTATCACGCTTTCGGTGCTTTCGTAAGTAGTATCTCTGCTTTGCCACGTATTGTGAC
GATGCATGACTTTGTTGTAGAGGCTGCTCAAGCTAAAGATAGTAAGTCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CCAAAACCTATCGATATATAGGAGTAACAGAGGCATCGGCACAACCAGCTAAGGCTGCAGCGGCGACTGCACCAACTAGC
CCTAATGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1VMD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.024

100

0.89
  comO Acinetobacter baylyi ADP1

71.369

97.967

0.699

Multiple sequence alignment