Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   DDU33_RS06200 Genome accession   NZ_CP029206
Coordinates   1264115..1265443 (-) Length   442 a.a.
NCBI ID   WP_108923798.1    Uniprot ID   A0A2U8FL73
Organism   Actinobacillus porcitonsillarum strain 9953L55     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1259115..1270443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDU33_RS06170 (DDU33_06170) - 1260146..1260784 (-) 639 WP_108923788.1 TIGR01621 family pseudouridine synthase -
  DDU33_RS06175 (DDU33_06175) - 1260832..1261464 (+) 633 WP_108923790.1 histidine phosphatase family protein -
  DDU33_RS06180 (DDU33_06180) - 1261508..1262131 (-) 624 WP_108923792.1 LysE family transporter -
  DDU33_RS06185 (DDU33_06185) - 1262136..1262609 (-) 474 WP_108923794.1 phosphatidylglycerophosphatase A -
  DDU33_RS06190 (DDU33_06190) thiL 1262618..1263577 (-) 960 WP_108923796.1 thiamine-phosphate kinase -
  DDU33_RS06195 (DDU33_06195) nusB 1263638..1264072 (-) 435 WP_005820144.1 transcription antitermination factor NusB -
  DDU33_RS06200 (DDU33_06200) comE 1264115..1265443 (-) 1329 WP_108923798.1 type IV pilus secretin PilQ Machinery gene
  DDU33_RS06205 (DDU33_06205) - 1265453..1265842 (-) 390 WP_108923800.1 pilus assembly protein PilP -
  DDU33_RS06210 (DDU33_06210) - 1265860..1266276 (-) 417 WP_244175303.1 hypothetical protein -
  DDU33_RS06215 (DDU33_06215) - 1266371..1266904 (-) 534 WP_108923804.1 hypothetical protein -
  DDU33_RS06220 (DDU33_06220) - 1266892..1267572 (-) 681 WP_108923806.1 hypothetical protein -
  DDU33_RS06225 (DDU33_06225) - 1267697..1270303 (+) 2607 WP_108923808.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 49022.15 Da        Isoelectric Point: 7.5517

>NTDB_id=291143 DDU33_RS06200 WP_108923798.1 1264115..1265443(-) (comE) [Actinobacillus porcitonsillarum strain 9953L55]
MKKWLYLLFFIPFSLFANQYSLILKNAPTPQILTYLAGESETNIVLNQDVDSFTTLRLENSPLETILKSITKINKLDLSK
EGNIYYVSEKKEENRVDIPQPPVKTAEIVVEKKPIIVTKTIKLQYAKASEVIDSLTKGNGSVLSDGGYIHFDERSNSLII
KDNPSSIKNMVSLIKELDKPTEQIAIEARIVTISSENLHELGVRWGMLSPNASHSRLGGTLEGNGVDSTKNLNVNFPVSG
GASAVLQVASINSKVLDLELSALEQENSVEIIASPRLLTTNKKLASIKQGTEIPYAIYDRKSETYDVEFKEAVLGLQVTP
HISKDNQILMDLVVTQNSQGQSVISSTSDNAAVSIDKQELNTQVFAKHGETIVLGGIFQHLKTKDSDRVPVLGSIPFLKH
LFSYQKDQISKRELVIFVTPYIIQANDINARPQKSKQNSKKL

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=291143 DDU33_RS06200 WP_108923798.1 1264115..1265443(-) (comE) [Actinobacillus porcitonsillarum strain 9953L55]
ATGAAAAAATGGCTTTATTTATTATTTTTTATTCCTTTTTCTCTTTTTGCGAACCAATATTCATTAATTTTAAAAAATGC
GCCTACGCCACAAATTCTTACTTATCTTGCAGGCGAATCCGAAACAAATATTGTACTGAATCAAGATGTGGATAGCTTTA
CCACGTTACGCTTAGAAAATAGTCCGCTTGAAACCATTTTAAAAAGTATCACAAAAATCAATAAACTTGATTTAAGTAAA
GAAGGCAACATTTACTACGTGAGTGAAAAGAAAGAAGAAAATCGTGTAGATATCCCTCAACCACCTGTAAAAACAGCTGA
AATCGTTGTTGAAAAAAAACCTATCATCGTAACAAAAACAATAAAACTACAATATGCCAAAGCATCTGAAGTCATTGATT
CCCTTACCAAAGGTAATGGCTCCGTTTTATCTGATGGCGGGTATATTCACTTTGATGAACGGAGTAATAGCTTAATTATC
AAAGATAATCCATCTTCTATAAAAAATATGGTGTCTCTTATCAAAGAGTTAGATAAACCTACCGAACAAATTGCCATTGA
AGCGAGAATCGTGACTATCAGTAGCGAAAATTTACATGAACTTGGCGTTCGTTGGGGTATGTTATCGCCAAATGCTTCCC
ATAGCCGATTGGGAGGAACACTTGAAGGAAATGGCGTGGATTCAACCAAAAATCTTAATGTGAATTTTCCTGTAAGTGGC
GGAGCCTCTGCGGTATTACAAGTTGCTTCAATCAATTCCAAAGTACTTGATCTTGAGCTCTCTGCACTAGAACAAGAAAA
CAGTGTTGAAATTATTGCAAGCCCTCGATTGCTTACGACTAATAAAAAATTAGCCAGCATCAAACAAGGTACCGAGATCC
CTTATGCCATTTATGATCGTAAATCTGAAACTTATGATGTGGAATTTAAAGAAGCGGTGCTTGGGTTACAAGTTACACCA
CATATCTCTAAAGATAACCAAATTTTGATGGATTTAGTCGTAACGCAAAATTCACAAGGACAGTCTGTTATAAGCTCTAC
TTCAGACAATGCTGCCGTCTCTATTGATAAACAAGAGTTAAATACGCAAGTTTTTGCCAAACATGGCGAAACCATTGTCT
TAGGAGGGATTTTCCAACATTTAAAAACAAAAGATAGTGATCGTGTCCCTGTATTAGGCTCAATTCCCTTTTTGAAACAT
CTCTTTAGTTATCAGAAAGATCAAATTAGTAAGCGAGAATTGGTTATTTTTGTTACACCTTATATTATTCAAGCAAATGA
TATAAATGCGCGACCTCAGAAATCCAAACAAAATAGCAAGAAACTATGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(262-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(118-183)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8FL73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

58.891

97.964

0.577
  comE Haemophilus influenzae Rd KW20

50.339

100

0.505
  comE Haemophilus influenzae 86-028NP

50.226

100

0.502
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.559

97.059

0.394
  pilQ Vibrio cholerae strain A1552

40.559

97.059

0.394
  pilQ Vibrio campbellii strain DS40M4

40.706

96.154

0.391

Multiple sequence alignment