Detailed information NTG0289972

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DDT09_RS12685
• Genome accession: NZ_CP029070
• Coordinates: 2591019..2591633 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus amyloliquefaciens strain ALB69
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2586019..2596633

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DDT09_RS12660 (DDT09_12660)	rpsT	2586322..2586588 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
DDT09_RS12665 (DDT09_12665)	holA	2586605..2587646 (-)	1042	Protein_2433	DNA polymerase III subunit delta	-
DDT09_RS20210	-	2587686..2587837 (-)	152	Protein_2434	hypothetical protein	-
DDT09_RS12670 (DDT09_12670)	-	2587878..2588012 (+)	135	WP_003152870.1	YqzM family protein	-
DDT09_RS12675 (DDT09_12675)	comEC	2588031..2590382 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DDT09_RS12680 (DDT09_12680)	-	2590383..2590952 (-)	570	WP_003152868.1	ComE operon protein 2	-
DDT09_RS12685 (DDT09_12685)	comEA	2591019..2591633 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
DDT09_RS12690 (DDT09_12690)	comER	2591692..2592513 (+)	822	WP_061862409.1	late competence protein ComER	-
DDT09_RS12695 (DDT09_12695)	-	2592582..2593319 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
DDT09_RS12700 (DDT09_12700)	rsfS	2593316..2593672 (-)	357	WP_007408260.1	ribosome silencing factor	-
DDT09_RS12705 (DDT09_12705)	yqeK	2593690..2594250 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DDT09_RS12710 (DDT09_12710)	-	2594240..2594809 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
DDT09_RS12715 (DDT09_12715)	yhbY	2594820..2595110 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DDT09_RS12720 (DDT09_12720)	aroE	2595104..2595946 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=289972 DDT09_RS12685 WP_014418419.1 2591019..2591633(-) (comEA) [Bacillus amyloliquefaciens strain ALB69]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=289972 DDT09_RS12685 WP_014418419.1 2591019..2591633(-) (comEA) [Bacillus amyloliquefaciens strain ALB69]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363