Detailed information NTG0289895

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAALB65_RS12830
• Genome accession: NZ_CP029069
• Coordinates: 2599138..2599752 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus amyloliquefaciens strain ALB65
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2594138..2604752

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAALB65_RS12805 (BAALB65_12805)	rpsT	2594441..2594707 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAALB65_RS12810 (BAALB65_12810)	holA	2594670..2595765 (-)	1096	Protein_2457	DNA polymerase III subunit delta	-
BAALB65_RS20210	-	2595805..2595956 (-)	152	Protein_2458	hypothetical protein	-
BAALB65_RS12815 (BAALB65_12815)	-	2595997..2596131 (+)	135	WP_003152870.1	YqzM family protein	-
BAALB65_RS12820 (BAALB65_12820)	comEC	2596150..2598501 (-)	2352	WP_109567080.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAALB65_RS12825 (BAALB65_12825)	-	2598502..2599071 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAALB65_RS12830 (BAALB65_12830)	comEA	2599138..2599752 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAALB65_RS12835 (BAALB65_12835)	comER	2599811..2600632 (+)	822	WP_109567081.1	late competence protein ComER	-
BAALB65_RS12840 (BAALB65_12840)	-	2600701..2601438 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
BAALB65_RS12845 (BAALB65_12845)	rsfS	2601435..2601791 (-)	357	WP_007408260.1	ribosome silencing factor	-
BAALB65_RS12850 (BAALB65_12850)	yqeK	2601809..2602369 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAALB65_RS12855 (BAALB65_12855)	-	2602359..2602928 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BAALB65_RS12860 (BAALB65_12860)	yhbY	2602939..2603229 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAALB65_RS12865 (BAALB65_12865)	aroE	2603223..2604065 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=289895 BAALB65_RS12830 WP_014418419.1 2599138..2599752(-) (comEA) [Bacillus amyloliquefaciens strain ALB65]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=289895 BAALB65_RS12830 WP_014418419.1 2599138..2599752(-) (comEA) [Bacillus amyloliquefaciens strain ALB65]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAAAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363