Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DB278_RS07200 Genome accession   NZ_CP028926
Coordinates   1494835..1495269 (+) Length   144 a.a.
NCBI ID   WP_083004804.1    Uniprot ID   A0A1Q1PRS7
Organism   Pasteurella multocida strain 20N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1489835..1500269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB278_RS07170 (DB278_07170) tadA 1489945..1490433 (-) 489 WP_108511809.1 tRNA adenosine(34) deaminase TadA -
  DB278_RS07175 (DB278_07175) - 1490460..1491311 (-) 852 WP_083004791.1 thymidylate synthase -
  DB278_RS07180 (DB278_07180) lgt 1491317..1492120 (-) 804 WP_108511810.1 prolipoprotein diacylglyceryl transferase -
  DB278_RS07185 (DB278_07185) - 1492130..1492924 (-) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  DB278_RS07190 (DB278_07190) rppH 1492927..1493523 (-) 597 WP_108511811.1 RNA pyrophosphohydrolase -
  DB278_RS07195 (DB278_07195) ampD 1494109..1494675 (-) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DB278_RS07200 (DB278_07200) pilA 1494835..1495269 (+) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  DB278_RS07205 (DB278_07205) pilB 1495269..1496654 (+) 1386 WP_083004806.1 GspE/PulE family protein Machinery gene
  DB278_RS07210 (DB278_07210) pilC 1496654..1497865 (+) 1212 WP_108511812.1 type II secretion system F family protein Machinery gene
  DB278_RS07215 (DB278_07215) - 1497865..1498590 (+) 726 WP_108511813.1 prepilin peptidase -
  DB278_RS07220 (DB278_07220) coaE 1498602..1499222 (+) 621 WP_096743227.1 dephospho-CoA kinase -
  DB278_RS07225 (DB278_07225) yacG 1499233..1499436 (+) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  DB278_RS07230 (DB278_07230) - 1499436..1499705 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15072.46 Da        Isoelectric Point: 8.8040

>NTDB_id=288618 DB278_RS07200 WP_083004804.1 1494835..1495269(+) (pilA) [Pasteurella multocida strain 20N]
MKKAIFFSIQKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=288618 DB278_RS07200 WP_083004804.1 1494835..1495269(+) (pilA) [Pasteurella multocida strain 20N]
ATGAAAAAAGCCATTTTCTTTTCTATTCAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q1PRS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

34.437

100

0.361

Multiple sequence alignment