Detailed information NTG0284460

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: C9888_RS13685
• Genome accession: NZ_CP028375
• Coordinates: 2742309..2742923 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain W1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2737309..2747923

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C9888_RS13660	rpsT	2737612..2737878 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
C9888_RS13665	holA	2737895..2738936 (-)	1042	Protein_2643	DNA polymerase III subunit delta	-
C9888_RS21495	-	2738976..2739127 (-)	152	Protein_2644	hypothetical protein	-
C9888_RS13670	-	2739168..2739302 (+)	135	WP_003152870.1	YqzM family protein	-
C9888_RS13675	comEC	2739321..2741672 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C9888_RS13680	-	2741673..2742242 (-)	570	WP_003152868.1	ComE operon protein 2	-
C9888_RS13685	comEA	2742309..2742923 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
C9888_RS13690	comER	2742982..2743803 (+)	822	WP_014418420.1	late competence protein ComER	-
C9888_RS13695	-	2743872..2744609 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
C9888_RS13700	rsfS	2744606..2744962 (-)	357	WP_007408260.1	ribosome silencing factor	-
C9888_RS13705	yqeK	2744980..2745540 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
C9888_RS13710	-	2745530..2746099 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
C9888_RS13715	yhbY	2746110..2746400 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
C9888_RS13720	aroE	2746394..2747236 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=284460 C9888_RS13685 WP_014418419.1 2742309..2742923(-) (comEA) [Bacillus velezensis strain W1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=284460 C9888_RS13685 WP_014418419.1 2742309..2742923(-) (comEA) [Bacillus velezensis strain W1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363