Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EB817_RS05060 Genome accession   NZ_CP033336
Coordinates   967360..968040 (-) Length   226 a.a.
NCBI ID   WP_002984709.1    Uniprot ID   A2REK0
Organism   Streptococcus pyogenes strain TSPY165     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 962360..973040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB817_RS05030 (EB817_05035) - 962599..963177 (+) 579 WP_002984698.1 CYTH domain-containing protein -
  EB817_RS05035 (EB817_05040) - 963317..964297 (+) 981 WP_002984700.1 ribose-phosphate diphosphokinase -
  EB817_RS05040 (EB817_05045) - 964294..965421 (+) 1128 WP_129304715.1 cysteine desulfurase family protein -
  EB817_RS05045 (EB817_05050) - 965411..965758 (+) 348 WP_002984704.1 DUF1831 domain-containing protein -
  EB817_RS05050 (EB817_05055) - 966009..966653 (+) 645 WP_002984705.1 redox-sensing transcriptional repressor Rex -
  EB817_RS05055 (EB817_05060) - 966663..967358 (+) 696 WP_129304717.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  EB817_RS05060 (EB817_05065) radC 967360..968040 (-) 681 WP_002984709.1 DNA repair protein RadC Machinery gene
  EB817_RS05065 (EB817_05070) - 968089..969216 (-) 1128 WP_002994399.1 AI-2E family transporter -
  EB817_RS05070 (EB817_05075) - 969396..970010 (-) 615 WP_030125941.1 SGNH/GDSL hydrolase family protein -
  EB817_RS05075 (EB817_05080) - 970173..971705 (-) 1533 WP_010922291.1 ClC family H(+)/Cl(-) exchange transporter -
  EB817_RS05080 (EB817_05085) aphA 971883..972614 (-) 732 WP_002984720.1 acid phosphatase AphA -

Sequence

Protein


Download         Length: 226 a.a.        Molecular weight: 25742.96 Da        Isoelectric Point: 7.3866

>NTDB_id=283093 EB817_RS05060 WP_002984709.1 967360..968040(-) (radC) [Streptococcus pyogenes strain TSPY165]
MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHVLELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKA
IEIKAMIELVSRILATDKTLTDSVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTIFIGTVRRSLAEPREILY
YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYSFREKSTLF

Nucleotide


Download         Length: 681 bp        

>NTDB_id=283093 EB817_RS05060 WP_002984709.1 967360..968040(-) (radC) [Streptococcus pyogenes strain TSPY165]
ATGTATTCTATAAAATGTGATGATAATAAAGCCATGCCAAGAGAACGTTTGATGCGACTAGGAGCAGAGTCTCTAAGTAA
TCAAGAATTATTAGCAATTTTATTACGAACAGGTAATAAAGAAAAGCATGTCTTAGAGCTGTCATCCTATCTTTTATCGC
ATTTAGACAGTCTGGCAGATTTTAAAAAGATGTCTTTGCAAGAATTACAACATTTGGCAGGTATAGGAAAAGTTAAAGCG
ATTGAAATTAAAGCTATGATTGAGTTGGTTTCCCGAATTTTAGCGACAGATAAGACATTAACTGATAGCGTATTAACCAG
TGTTCAGGTCGCTGAAAAAATGATGGCAGCTTTAGGAGATAAAAAACAAGAGCATTTAGTCGTATTGTATTTAGATAATC
AAAATCGTATTATTGAAGAAAAAACTATTTTTATTGGGACTGTCCGACGTTCACTTGCAGAACCAAGAGAAATTTTATAC
TATGCCTGTAAAAATATGGCGACTAGTCTCATTGTTATTCATAATCATCCTTCAGGAAATATTGAACCTAGTTCTAACGA
TTATTGCTTTACTGAAAAAATAAAACGATCATGTGAAGATTTAGGCATTATCTGTCTAGATCACATTATCGTTAGCTATA
AAGATTATTATAGTTTTCGAGAAAAATCAACCCTTTTTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(107-222)

UPF0758 N-terminal (UPF0758_N)

(10-81)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2REK0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Streptococcus pneumoniae TIGR4

57.965

100

0.58
  radC Streptococcus pneumoniae R6

57.522

100

0.575
  radC Streptococcus pneumoniae D39

57.522

100

0.575
  radC Streptococcus gordonii str. Challis substr. CH1

55.752

100

0.558
  ysxA/radC Bacillus subtilis subsp. subtilis str. 168

43.119

96.46

0.416
  radC Haemophilus influenzae Rd KW20

37.9

96.903

0.367