Detailed information NTG0282533

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: C7M18_RS19600
• Genome accession: NZ_CP028204
• Coordinates: 4020545..4021159 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain SRCM102755
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4015545..4026159

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7M18_RS19570 (C7M18_03940)	rpsT	4015848..4016114 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
C7M18_RS19575 (C7M18_03941)	holA	4016077..4017172 (-)	1096	Protein_3846	DNA polymerase III subunit delta	-
C7M18_RS19580	-	4017212..4017363 (-)	152	Protein_3847	hypothetical protein	-
C7M18_RS19585	-	4017404..4017538 (+)	135	WP_003152870.1	YqzM family protein	-
C7M18_RS19590 (C7M18_03942)	comEC	4017557..4019908 (-)	2352	WP_109567080.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C7M18_RS19595 (C7M18_03943)	-	4019909..4020478 (-)	570	WP_003152868.1	ComE operon protein 2	-
C7M18_RS19600 (C7M18_03944)	comEA	4020545..4021159 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
C7M18_RS19605 (C7M18_03945)	comER	4021218..4022039 (+)	822	WP_109567081.1	late competence protein ComER	-
C7M18_RS19610 (C7M18_03946)	-	4022108..4022845 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
C7M18_RS19615 (C7M18_03947)	rsfS	4022842..4023198 (-)	357	WP_007408260.1	ribosome silencing factor	-
C7M18_RS19620 (C7M18_03948)	yqeK	4023216..4023776 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
C7M18_RS19625 (C7M18_03949)	-	4023766..4024335 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
C7M18_RS19630 (C7M18_03950)	yhbY	4024346..4024636 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
C7M18_RS19635 (C7M18_03951)	aroE	4024630..4025472 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=282533 C7M18_RS19600 WP_014418419.1 4020545..4021159(-) (comEA) [Bacillus velezensis strain SRCM102755]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=282533 C7M18_RS19600 WP_014418419.1 4020545..4021159(-) (comEA) [Bacillus velezensis strain SRCM102755]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAAAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363