Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C4F17_RS24790 Genome accession   NZ_CP027773
Coordinates   5293730..5295463 (-) Length   577 a.a.
NCBI ID   WP_081267928.1    Uniprot ID   -
Organism   Variovorax sp. PMC12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5288730..5300463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4F17_RS24770 (C4F17_24770) - 5289326..5290819 (-) 1494 WP_106937022.1 MBOAT family protein -
  C4F17_RS24775 (C4F17_24775) coaE 5290889..5291497 (-) 609 WP_199851988.1 dephospho-CoA kinase -
  C4F17_RS24780 (C4F17_24780) - 5291509..5292474 (-) 966 WP_106937023.1 A24 family peptidase -
  C4F17_RS24785 (C4F17_24785) pilC 5292474..5293703 (-) 1230 WP_081267927.1 type II secretion system F family protein Machinery gene
  C4F17_RS24790 (C4F17_24790) pilB 5293730..5295463 (-) 1734 WP_081267928.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C4F17_RS24800 (C4F17_24800) - 5295707..5296699 (-) 993 WP_106937024.1 polyprenyl synthetase family protein -
  C4F17_RS24805 (C4F17_24805) rplU 5296883..5297194 (+) 312 WP_057592097.1 50S ribosomal protein L21 -
  C4F17_RS24810 (C4F17_24810) rpmA 5297207..5297467 (+) 261 WP_081267930.1 50S ribosomal protein L27 -
  C4F17_RS24815 (C4F17_24815) obgE 5297539..5298615 (+) 1077 WP_081267931.1 GTPase ObgE -
  C4F17_RS24820 (C4F17_24820) proB 5298690..5299829 (+) 1140 WP_106937025.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62524.61 Da        Isoelectric Point: 5.7372

>NTDB_id=280498 C4F17_RS24790 WP_081267928.1 5293730..5295463(-) (pilB) [Variovorax sp. PMC12]
MAAAELPVKESAQIALPGLARALISAGKLAAKTAEDIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
NSIVGSEFEFDEVTPDTSGDANDQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHNYRVRFRVDGELREVASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKAR
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCRAPADVPR
EALLDAGFQEEQLDGSWKPYRPVGCSACSGGYKGRVGIYQVMPISEAIQEIILRDGSALDIAQQSEREGVRSLRQSGLRK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=280498 C4F17_RS24790 WP_081267928.1 5293730..5295463(-) (pilB) [Variovorax sp. PMC12]
ATGGCCGCTGCCGAACTTCCAGTCAAAGAATCCGCGCAGATAGCGCTTCCTGGGCTCGCGCGCGCGCTGATTTCGGCCGG
CAAGCTGGCCGCCAAGACGGCGGAAGACATTTATCAGAAGTCGCTGAGCGGCCGCACCAGCTTCATCGCGGAACTGACCG
GCACTGGCGCCGTGTCGGCCGCGGATCTTGCCCATACCCTTTCCAGCGCATTCGGTGCCCCGCTGCTCGACCTCGACGCC
ATCGACCACCAGCGCCTGCCCAAGGACCTGCTCGACCCGAAGCTCTGCCACGCCTACCGCATCGTCGTTCTCAGCAAGCG
CAACAACCGGCTGATCGTTGCCACGGCAGACCCGTCGGACCAGCAGGCGGCGGAGAAGATCAAGTTCGCGTCGCAGATGG
GCGTCGACTGGGTCATCGCGGAATACGACAAGCTGTCGCGCATGATCGAAGCCGCCGCCGTGAGCGCGGCGGAGACCATC
AACAGCATCGTCGGCAGCGAGTTCGAATTCGACGAGGTCACGCCCGACACCTCGGGCGACGCCAATGACCAGGCGATCGC
CGAAGTCGAAGACGCGCCGGTGGTGCGTTTCCTCCACAAGATGCTGCTCGACGCCGTCAGCATGCGGGCATCGGACATCC
ATTTCGAGCCCTACGAGCACAACTACCGTGTGCGTTTCCGCGTCGACGGCGAACTGCGCGAGGTCGCCAGCCCGCCGACC
GTCATCAAGGACAAGCTCGCCTCCCGCATCAAGGTGATTTCGCGGCTCGACATCTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGCCCCGACCGCGTGATCGACTTTCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGGTTCGTATCCTCGACCCCAGCAGCGCGCGCCTGGGCATCGATGCGCTGGGCTACGACGCCGACGAGAAGGCGCGG
CTTCTGCATGCCATCGGCCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACGGGTTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCGGGCGTGAACATCGCAACGGCGGAAGACCCGTCGGAAATCAACCTGCCTGGCG
TCAACCAGGTCAACGTCAACGAGCGCGCGGGGCTGACTTTCGCCGCCGCGCTGCGCGCGTTCCTGCGGCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAGACAGCGGATATTTCGATCAAGGCGGCGCAGACGGGCCACCTGGTGCT
CTCGACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAACATCGCAT
CGAGCGTGATCCTGATCACCGCGCAGCGCCTTGCGCGGCGGCTGTGCACCGTCTGCCGCGCGCCCGCGGACGTACCGCGC
GAAGCCCTCCTCGACGCGGGATTCCAGGAAGAACAACTGGACGGCAGCTGGAAGCCTTACCGCCCGGTCGGCTGCTCGGC
ATGCAGCGGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCTCGGAAGCGATCCAGGAAATCATCCTGC
GCGACGGCAGCGCACTCGACATCGCACAGCAGTCCGAGAGGGAAGGCGTGCGCTCGCTGCGCCAGTCCGGCCTGCGCAAG
GTCATGCAGGGCCTGACTTCGCTCGAAGAAGTCGTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.713

100

0.539
  pilB Acinetobacter baumannii D1279779

53.832

97.227

0.523
  pilF Neisseria gonorrhoeae MS11

52.575

97.574

0.513
  pilB Legionella pneumophila strain ERS1305867

48.571

97.054

0.471
  pilB Vibrio cholerae strain A1552

46.726

97.92

0.458
  pilB Vibrio campbellii strain DS40M4

45.23

98.094

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

45.438

96.88

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.7

94.107

0.383
  pilF Thermus thermophilus HB27

37.478

98.96

0.371

Multiple sequence alignment