Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   SPYM_RS02495 Genome accession   NC_009332
Coordinates   493325..495223 (+) Length   632 a.a.
NCBI ID   WP_011888669.1    Uniprot ID   -
Organism   Streptococcus pyogenes str. Manfredo     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 488325..500223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM_RS02480 (SpyM50453) fmt 490303..491238 (+) 936 WP_002988958.1 methionyl-tRNA formyltransferase -
  SPYM_RS02485 (SpyM50454) rsmB 491228..492550 (+) 1323 WP_011184816.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  SPYM_RS02490 (SpyM50455) - 492588..493328 (+) 741 WP_002983660.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  SPYM_RS02495 (SpyM50456) pknB 493325..495223 (+) 1899 WP_011888669.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  SPYM_RS02500 (SpyM50457) liaF 495346..496038 (+) 693 WP_011888670.1 cell wall-active antibiotics response protein LiaF -
  SPYM_RS02505 (SpyM50458) - 496035..497039 (+) 1005 WP_002991890.1 sensor histidine kinase -
  SPYM_RS02510 (SpyM50459) - 497032..497673 (+) 642 WP_011888671.1 response regulator transcription factor -
  SPYM_RS02515 (SpyM50460) - 497710..499110 (+) 1401 WP_011888672.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  SPYM_RS02520 (SpyM50461) - 499110..499487 (+) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 632 a.a.        Molecular weight: 68805.04 Da        Isoelectric Point: 7.2899

>NTDB_id=27853 SPYM_RS02495 WP_011888669.1 493325..495223(+) (pknB) [Streptococcus pyogenes str. Manfredo]
MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVLRTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIG
EEDGQQFLVMEYVDGADLKRYIQNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKDGVVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIEENHNVP
QALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYNRSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESR
LDQTNQTDALQPPTKKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDVGLKVGKIR
QIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDYQEAMNSLIETYGVPKSKIKIERIVTNEYPE
NTVISQSPSAGDKFNPNGKSKITLSVAVSDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSS
KAIVSGQSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSPSETTAQTP

Nucleotide


Download         Length: 1899 bp        

>NTDB_id=27853 SPYM_RS02495 WP_011888669.1 493325..495223(+) (pknB) [Streptococcus pyogenes str. Manfredo]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGCATTCTGAAATCTATTGGCCGCGGTGGTATGGCGGATGTTTA
TTTAGCAAATGATTTGATCTTGGATAATGAAGACGTTGCAATCAAGGTCTTGCGTACCAATTATCAAACAGATCAGGTAG
CAGTTGCGCGTTTCCAACGAGAAGCGCGGGCCATGGCTGAATTGAACCATCCCAATATTGTTGCCATCCGGGATATAGGT
GAAGAAGACGGACAGCAATTTTTAGTAATGGAATATGTGGATGGTGCTGACCTAAAGAGATACATTCAAAATCATGCTCC
ATTATCTAATAATGAAGTGGTTAGAATTATGGAAGAAGTCCTTTCTGCTATGACTTTAGCCCACCAAAAAGGAATTGTAC
ACAGAGATTTAAAACCTCAAAATATCCTACTAACTAAGGATGGTGTTGTCAAAGTAACTGATTTCGGCATCGCAGTAGCC
TTTGCAGAAACAAGCTTGACACAAACTAATTCGATGTTAGGCAGTGTTCATTACTTGTCTCCAGAACAGGCTCGCGGCTC
CAAAGCGACGATTCAAAGTGATATTTATGCGATGGGGATTATGCTCTTTGAGATGTTGACAGGCCATATCCCTTATGATG
GCGATAGTGCTGTTACGATTGCCTTGCAACATTTTCAAAAGCCTCTTCCATCTATTATCGAGGAGAACCACAATGTGCCA
CAAGCTTTGGAGAATGTTGTTATTCGAGCAACAGCCAAGAAATTAAGTGATCGTTACGGGTCAACCTTTGAAATGAGTCG
TGACTTAATGACGGCGCTTAGTTATAATCGTAGTCGGGAGCGTAAGATTGTCTTTGAGGATGTTGAAAGTACCAAACCCC
TCCCCAAAGTGGCCTCAGGCCCCACCGCTTCTGTAAAATTGTCTCCCCCTACCCCAACAGTGTTAACACAGGAAAGTCGA
TTAGATCAAACTAATCAAACAGATGCTTTACAGCCCCCCACCAAAAAGAAAAAAAGTGGTCGTTTTTTAGGTACTTTATT
CAAAATTCTTTTTTCTTTCTTTATTGTAGGTGTAGCACTCTTTACTTATCTTATACTAACTAAACCAACTTCTGTGAAAG
TTCCTAATGTAGCAGGCACTAGTCTTAAAGTTGCCAAACAAGAACTGTATGATGTTGGGCTAAAAGTGGGTAAAATCAGG
CAAATTGAGAGTGATACGGTTGCTGAGGGAAATGTAGTTAGAACAGATCCTAAAGCAGGAACAGCTAAGAGGCAAGGCTC
AAGCATTACGCTTTATGTGTCAATTGGAAACAAAGGTTTTGACATGGAAAACTACAAAGGATTAGATTATCAAGAAGCTA
TGAATAGTTTGATAGAAACTTATGGTGTTCCAAAATCAAAAATCAAAATTGAGCGCATTGTAACTAATGAATATCCTGAA
AATACAGTCATCAGTCAATCGCCAAGTGCGGGTGATAAATTTAATCCAAACGGAAAGTCTAAAATTACGCTCAGTGTTGC
TGTTAGTGATACGATCACTATGCCTATGGTAACAGAATATAGTTATGCAGATGCAGTCAATACCTTAACAGCTTTAGGTA
TAGATGCATCTAGAATAAAAGCTTATGTGCCAAGCTCTAGCTCAGCAACGGGCTTTGTGCCAATTCATTCTCCTAGTTCT
AAAGCTATTGTCAGTGGTCAATCTCCTTACTATGGAACGTCTTTGAGTCTGTCTGACAAAGGAGAGATTAGTCTTTACCT
TTATCCAGAAGAAACACACTCTTCTAGTAGCTCATCGAGTTCAACGTCAAGTTCAAACAGTTCTTCAACAAATGATAGTA
CTGCACCAGGTAGCAACACTGAATTAAGCCCATCAGAAACTACTGCTCAAACACCTTAA

Domains

Predicted by InterproScan.

PASTA domain (PASTA)

(372-434)

PASTA domain (PASTA)

(441-506)

PASTA domain (PASTA)

(512-588)

Protein kinase domain (Pkinase)

(12-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

65.593

93.354

0.612
  stkP/pknB Streptococcus salivarius strain HSISS4

61.681

94.146

0.581
  stkP Streptococcus pneumoniae TIGR4

57.364

81.646

0.468
  stkP Streptococcus pneumoniae D39

57.171

81.646

0.467
  stkP Streptococcus pneumoniae R6

57.171

81.646

0.467
  stkP Streptococcus pneumoniae Rx1

56.977

81.646

0.465

Multiple sequence alignment