Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   D8S77_RS00665 Genome accession   NZ_CP032700
Coordinates   102893..103927 (+) Length   344 a.a.
NCBI ID   WP_233436572.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY556     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97893..108927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8S77_RS00655 (D8S77_00655) - 101561..101926 (+) 366 WP_002986560.1 DUF1033 family protein -
  D8S77_RS00660 (D8S77_00660) comGA 102019..102957 (+) 939 WP_063812149.1 competence type IV pilus ATPase ComGA Machinery gene
  D8S77_RS00665 (D8S77_00665) comGB 102893..103927 (+) 1035 WP_233436572.1 competence type IV pilus assembly protein ComGB Machinery gene
  D8S77_RS00670 (D8S77_00670) comGC 103929..104255 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  D8S77_RS00675 (D8S77_00675) comGD 104230..104658 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD Machinery gene
  D8S77_RS00680 (D8S77_00680) comGE 104615..104899 (+) 285 WP_032465969.1 competence type IV pilus minor pilin ComGE Machinery gene
  D8S77_RS00685 (D8S77_00685) comGF 104892..105326 (+) 435 WP_129321637.1 competence type IV pilus minor pilin ComGF Machinery gene
  D8S77_RS00690 (D8S77_00690) comGG 105310..105636 (+) 327 WP_129321638.1 competence type IV pilus minor pilin ComGG -
  D8S77_RS00695 (D8S77_00695) comGH 105734..106687 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  D8S77_RS00700 (D8S77_00700) - 106746..107942 (+) 1197 WP_010921803.1 acetate kinase -
  D8S77_RS00705 (D8S77_00705) - 108129..108437 (+) 309 WP_111677000.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39688.20 Da        Isoelectric Point: 9.7085

>NTDB_id=277685 D8S77_RS00665 WP_233436572.1 102893..103927(+) (comGB) [Streptococcus pyogenes strain TSPY556]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIEITLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=277685 D8S77_RS00665 WP_233436572.1 102893..103927(+) (comGB) [Streptococcus pyogenes strain TSPY556]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAATAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Streptococcus mutans UA140

53.982

98.547

0.532
  comGB Streptococcus mutans UA159

53.982

98.547

0.532
  comGB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404