Detailed information NTG0275982

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C6N18_RS05120
• Genome accession: NZ_CP027246
• Coordinates: 881527..882645 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain WCHAB005078
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 876527..887645

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6N18_RS05085 (C6N18_05085)	-	877356..878204 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
C6N18_RS05090 (C6N18_05090)	-	878457..878912 (+)	456	WP_000782976.1	bacteriohemerythrin	-
C6N18_RS05100 (C6N18_05100)	-	879065..880132 (+)	1068	WP_000092403.1	hypothetical protein	-
C6N18_RS05105 (C6N18_05105)	-	880132..880455 (+)	324	WP_000442585.1	RnfH family protein	-
C6N18_RS05110 (C6N18_05110)	-	880510..880908 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
C6N18_RS05115 (C6N18_05115)	fur	881020..881457 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
C6N18_RS05120 (C6N18_05120)	pilU	881527..882645 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C6N18_RS05125 (C6N18_05125)	pilT	882673..883710 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
C6N18_RS05130 (C6N18_05130)	-	883837..884529 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=275982 C6N18_RS05120 WP_000347036.1 881527..882645(-) (pilU) [Acinetobacter baumannii strain WCHAB005078]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=275982 C6N18_RS05120 WP_000347036.1 881527..882645(-) (pilU) [Acinetobacter baumannii strain WCHAB005078]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392