Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AM442_RS01200 Genome accession   NZ_CP026711
Coordinates   225149..227314 (-) Length   721 a.a.
NCBI ID   WP_001017038.1    Uniprot ID   A0A427R068
Organism   Acinetobacter baumannii strain AR_0063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220149..232314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM442_RS01185 (AM442_01185) - 222566..223441 (-) 876 WP_000218798.1 hypothetical protein -
  AM442_RS01190 (AM442_01190) aroB 223457..224539 (-) 1083 WP_001194002.1 3-dehydroquinate synthase -
  AM442_RS01195 (AM442_01195) aroK 224568..225137 (-) 570 WP_000643712.1 shikimate kinase AroK -
  AM442_RS01200 (AM442_01200) pilQ 225149..227314 (-) 2166 WP_001017038.1 type IV pilus secretin PilQ family protein Machinery gene
  AM442_RS01205 (AM442_01205) pilP 227377..227904 (-) 528 WP_000695058.1 pilus assembly protein PilP Machinery gene
  AM442_RS01210 (AM442_01210) pilO 227915..228655 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  AM442_RS01215 (AM442_01215) pilN 228652..229293 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  AM442_RS01220 (AM442_01220) pilM 229293..230351 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 721 a.a.        Molecular weight: 77921.31 Da        Isoelectric Point: 6.5404

>NTDB_id=271924 AM442_RS01200 WP_001017038.1 225149..227314(-) (pilQ) [Acinetobacter baumannii strain AR_0063]
MNHVFRQFSMGAVAIAIMQAASAQVSMTNIVPMQIAGQGTEIRVMFNGLPPQPQAYQLENPSRLILDFDKAQQGLKQSKI
SVATNETSSVDVTSDDQRSRLTVNLKDAGAFTTRVEGNTFILKINSAQTSNKPLPVVSAQPQGVSNIGFQRGSQGEGLVV
VDLLGSNTPVDVQQQGSKVVIRTIGTKIPTHLARRLNVNDFATPVSSIDAYNDKGVGVITIQSSGSYEYMAYQAENKLTI
SLKRPQDKNVTSLYKTPNYSGNKLSLDFQDIEVRRVLQLLADFTGINMVAADSVQGNITLRLKDVPWDQALDIILKTKNL
DKRRNGNVIWIAPVAELIKAEEEEAKAVAQSVKLAPLQTEYIQLKYAKAGDIMGLITQGSNNSNGLQHTSGGGTSTSTNL
NTGVDSLGNNVGSLLSPRGTITQDDRTNTLIINDTAQSIDQIRKMIDLLDVQVKQVMVEARIVRASTSFTKELGVKWGIL
SQGITNNNNLLVGGSETTLWNLREPKKDETTGGYKYTIERPDNLNVDLGVSNPAGSIAFGLISMSDFMLDLELSALQADG
YGEVISTPKVMTADKQTAKVATGQQVPYQSTTNSAAGSTATTSFKDALLSLNVTPSITPDGKIQMKLDISKDSVAGAAPN
GELILNKNNINTNVLVDNGETVILGGVFEQTTMNSQTKVPFFGDIPVVGRLFRKDVKSDDKQELLIFVTPRIVNDTLARN
H

Nucleotide


Download         Length: 2166 bp        

>NTDB_id=271924 AM442_RS01200 WP_001017038.1 225149..227314(-) (pilQ) [Acinetobacter baumannii strain AR_0063]
ATGAATCACGTATTCCGTCAGTTTTCTATGGGGGCTGTAGCTATTGCGATTATGCAGGCTGCATCTGCACAGGTCAGCAT
GACCAATATTGTACCAATGCAAATTGCAGGTCAGGGCACAGAAATTAGAGTCATGTTTAATGGCTTGCCACCACAACCAC
AAGCTTACCAACTTGAAAATCCTTCACGTTTAATACTTGATTTTGATAAGGCACAGCAAGGTTTAAAACAGTCTAAAATT
AGTGTTGCAACTAATGAGACAAGTTCTGTAGATGTTACTTCAGATGACCAGCGTTCACGTTTAACTGTAAACCTAAAAGA
TGCTGGAGCATTTACAACACGAGTAGAAGGTAATACCTTTATTTTAAAAATTAATTCTGCTCAAACAAGTAATAAGCCGC
TACCTGTGGTCTCAGCACAACCACAAGGTGTTTCAAATATTGGCTTTCAACGTGGTAGTCAGGGAGAAGGCCTGGTTGTT
GTAGATCTTTTAGGAAGTAATACGCCTGTAGATGTTCAGCAGCAGGGTAGTAAAGTCGTTATAAGAACAATAGGTACAAA
AATCCCGACACATTTAGCACGTCGCTTAAATGTAAATGATTTTGCAACCCCGGTTTCAAGTATTGATGCTTATAATGATA
AAGGGGTTGGTGTAATAACTATCCAGTCCTCTGGTAGCTATGAATATATGGCCTATCAGGCAGAGAATAAGCTCACGATC
AGTTTGAAACGTCCCCAAGATAAAAATGTTACCTCTTTATATAAAACGCCAAATTATTCAGGAAATAAACTTTCATTAGA
CTTCCAAGATATTGAAGTACGCCGTGTGTTGCAATTACTTGCTGATTTTACAGGCATCAATATGGTAGCTGCCGATAGTG
TACAAGGTAATATCACACTACGTTTAAAAGATGTACCTTGGGATCAGGCTCTTGATATTATTTTAAAAACTAAAAATCTT
GATAAACGCCGTAATGGCAATGTGATTTGGATTGCTCCAGTTGCAGAATTGATTAAGGCGGAAGAAGAAGAAGCGAAAGC
TGTTGCACAAAGTGTCAAACTTGCACCCTTACAGACTGAGTATATTCAACTGAAGTATGCTAAAGCTGGCGATATTATGG
GTCTCATTACACAAGGCTCAAATAATAGTAATGGTTTACAGCATACTTCTGGTGGAGGGACTTCAACTTCAACCAATTTA
AATACTGGAGTTGATAGTCTAGGCAATAATGTGGGTAGTTTGCTTAGCCCACGTGGCACTATTACCCAAGATGACCGAAC
TAATACTCTTATTATTAATGATACTGCTCAGTCAATTGATCAAATTCGTAAAATGATTGACTTGCTTGATGTGCAAGTTA
AACAAGTTATGGTTGAAGCACGTATTGTGAGAGCCTCTACGTCATTTACCAAAGAACTTGGTGTGAAGTGGGGTATTCTG
TCGCAAGGTATTACCAATAACAACAATTTGCTGGTTGGCGGTAGTGAAACAACTTTATGGAATTTACGCGAACCTAAAAA
AGATGAAACTACAGGTGGGTATAAATATACGATTGAGCGCCCAGATAACTTAAACGTGGATTTAGGAGTTTCTAATCCTG
CAGGAAGTATTGCTTTTGGCTTGATTAGTATGTCTGACTTTATGTTAGATCTTGAATTGTCTGCTTTACAAGCGGATGGT
TATGGTGAGGTTATTTCTACCCCTAAAGTCATGACTGCCGATAAACAAACAGCGAAAGTTGCAACTGGTCAACAAGTTCC
ATATCAATCTACAACTAACTCTGCAGCAGGTTCAACTGCTACAACATCTTTTAAAGATGCTTTGCTGAGTTTAAATGTGA
CACCAAGTATTACACCTGATGGCAAGATTCAAATGAAGTTGGATATTTCTAAGGATTCTGTAGCGGGTGCGGCTCCAAAT
GGAGAGTTGATTTTAAATAAAAATAATATCAACACCAATGTATTGGTAGATAATGGCGAAACTGTCATTTTGGGTGGTGT
TTTTGAGCAAACGACTATGAATTCACAAACCAAAGTTCCTTTCTTTGGTGATATTCCTGTTGTTGGGCGTTTATTTAGAA
AAGATGTAAAGTCTGATGATAAACAAGAGTTGCTGATTTTCGTTACACCACGAATTGTTAATGACACTTTAGCGAGAAAT
CATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(291-333)

Bacterial type II/III secretion system short domain (Secretin_N)

(360-455)

AMIN domain (AMIN)

(39-121)

Bacterial type II and III secretion system protein (Secretin)

(555-713)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A427R068

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

99.861

100

0.999
  pilQ Acinetobacter baumannii strain A118

99.723

100

0.997
  comQ Acinetobacter baylyi ADP1

75.242

100

0.755
  pilQ Pseudomonas aeruginosa PAK

40.559

99.168

0.402

Multiple sequence alignment