Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AM442_RS01210 Genome accession   NZ_CP026711
Coordinates   227915..228655 (-) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain AR_0063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 222915..233655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM442_RS01190 (AM442_01190) aroB 223457..224539 (-) 1083 WP_001194002.1 3-dehydroquinate synthase -
  AM442_RS01195 (AM442_01195) aroK 224568..225137 (-) 570 WP_000643712.1 shikimate kinase AroK -
  AM442_RS01200 (AM442_01200) pilQ 225149..227314 (-) 2166 WP_001017038.1 type IV pilus secretin PilQ family protein Machinery gene
  AM442_RS01205 (AM442_01205) pilP 227377..227904 (-) 528 WP_000695058.1 pilus assembly protein PilP Machinery gene
  AM442_RS01210 (AM442_01210) pilO 227915..228655 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  AM442_RS01215 (AM442_01215) pilN 228652..229293 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  AM442_RS01220 (AM442_01220) pilM 229293..230351 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  AM442_RS01225 (AM442_01225) ponA 230513..233068 (+) 2556 WP_000736683.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=271926 AM442_RS01210 WP_000076101.1 227915..228655(-) (pilO) [Acinetobacter baumannii strain AR_0063]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=271926 AM442_RS01210 WP_000076101.1 227915..228655(-) (pilO) [Acinetobacter baumannii strain AR_0063]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCAAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGAGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAGGATAACAAGTCTGATATTCCTGTACTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment