Detailed information NTG0027147

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MAQU_RS04190
• Genome accession: NC_008740
• Coordinates: 938692..939756 (+)
• Length: 354 a.a.
• NCBI ID: WP_011784345.1
• Uniprot ID: A0A368USH3
• Organism: Marinobacter nauticus VT8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 933692..944756

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MAQU_RS04175 (Maqu_0823)	rpmE	934126..934347 (+)	222	WP_011784342.1	50S ribosomal protein L31	-
MAQU_RS04180 (Maqu_0824)	-	934562..935827 (+)	1266	WP_011784343.1	malic enzyme-like NAD(P)-binding protein	-
MAQU_RS04185 (Maqu_0825)	-	935914..938454 (-)	2541	WP_011784344.1	penicillin-binding protein 1A	-
MAQU_RS04190 (Maqu_0826)	pilM	938692..939756 (+)	1065	WP_011784345.1	pilus assembly protein PilM	Machinery gene
MAQU_RS04195 (Maqu_0827)	-	939756..940319 (+)	564	WP_011784346.1	PilN domain-containing protein	-
MAQU_RS04200 (Maqu_0828)	-	940319..940930 (+)	612	WP_011784347.1	type 4a pilus biogenesis protein PilO	-
MAQU_RS04205 (Maqu_0829)	-	940933..941475 (+)	543	WP_011784348.1	pilus assembly protein PilP	-
MAQU_RS04210 (Maqu_0830)	pilQ	941541..943604 (+)	2064	WP_236078963.1	type IV pilus secretin PilQ family protein	Machinery gene
MAQU_RS04215 (Maqu_0831)	aroK	943743..944333 (+)	591	WP_041656175.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37993.03 Da
• Isoelectric Point: 4.1824

>NTDB_id=27147 MAQU_RS04190 WP_011784345.1 938692..939756(+) (pilM) [Marinobacter nauticus VT8]
MFGLPGKKSSAVLGVDVSSSSVKLLELSKHGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTSVKQVAV
AVSGSAVITKVIQMDGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSESNPDQVDVLLAACRKENVDIREDALEI
AGLTTKIVDVEAYALERAYSLIEPQLDSQGEELVVAIVDIGATMTTLSVLAEGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYNEEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FANMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=27147 MAQU_RS04190 WP_011784345.1 938692..939756(+) (pilM) [Marinobacter nauticus VT8]
GTGTTCGGATTACCAGGAAAAAAATCCAGTGCCGTCCTTGGAGTGGATGTAAGCTCCAGTTCGGTCAAGCTGCTAGAGCT
GTCAAAACATGGCGATCGATACAAGGTCGAGAGCTATGCGGTAGAGCCGTTGCCGGCCAATGCAGTTGTTGAAAAAAACA
TCACAGACGTCGAAGCGGTTGGGGAAGTGCTCAAACGTGTCGCGTCCAAGTCACGTACCAGTGTGAAGCAGGTGGCTGTT
GCTGTTTCCGGCTCGGCGGTGATCACCAAGGTTATCCAGATGGACGGTGGTCTCAACGAGTTTGAAATGGAAGACCAGAT
TGCGCTGGAAGCCGATCAGTACATTCCTTACCCCCTCGATGAAGTGGCGATCGATTTCGAAGTCCAGGGCCCGTCTGAAT
CCAACCCGGATCAGGTGGATGTGCTGCTGGCGGCCTGCCGTAAGGAAAACGTCGACATCCGCGAAGATGCCCTGGAAATT
GCAGGGCTGACAACCAAGATTGTCGACGTGGAAGCCTATGCTCTGGAGCGGGCTTACAGCCTGATTGAGCCTCAGCTCGA
CTCTCAGGGAGAAGAGTTGGTGGTTGCCATCGTTGATATTGGCGCGACCATGACAACTCTGAGTGTGCTGGCAGAGGGTA
AGACGGTCTATACCCGCGAGCAGATTTTTGGCGGAAAACAGCTGACGGAAGAAATTCAACGTCGTTACGGGTTGTCGCTC
GAAGAAGCTGGTCTGGCCAAGAAGCAGGGTGGTTTGCCAGACGATTACAACGAGGAAGTCCTGACCCCGTTCCGGGAAGC
GGTTGTTCAGCAAGTGGCGCGGGCGCTGCAGTTTTTCTTCGGCGCGAGTCAGTACAACGCGGTGGATTACGTGGTGCTTG
CCGGAGGAACAGCGTCGATTCAGGGGCTCACGGAAATGGTGGAAGAGAAAACTGGAACGCCAACCCTGGTGGCCAATCCG
TTTGCCAATATGGCGGTGGGCTCCCGGGTTAACGCCTCTGCGCTCAGCAATGATGCCCCCTCGTTGATGATTGCCTGCGG
TCTGGCAATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A368USH3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.672	100	0.537
comM	Acinetobacter nosocomialis M2	53.672	100	0.537
comM	Acinetobacter baylyi ADP1	51.695	100	0.517
pilM	Legionella pneumophila strain ERS1305867	45.198	100	0.452