Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   C0W57_RS04245 Genome accession   NZ_CP025939
Coordinates   753040..753477 (+) Length   145 a.a.
NCBI ID   WP_007612572.1    Uniprot ID   -
Organism   Bacillus velezensis strain 10075     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 748040..758477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0W57_RS04220 (C0W57_04220) - 748053..749354 (-) 1302 WP_029973873.1 hemolysin family protein -
  C0W57_RS04225 (C0W57_04225) - 749500..750450 (+) 951 WP_014418379.1 magnesium transporter CorA family protein -
  C0W57_RS04230 (C0W57_04230) comGA 750643..751713 (+) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  C0W57_RS04235 (C0W57_04235) comGB 751700..752737 (+) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  C0W57_RS04240 (C0W57_04240) comGC 752742..753050 (+) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  C0W57_RS04245 (C0W57_04245) comGD 753040..753477 (+) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  C0W57_RS04250 (C0W57_04250) comGE 753461..753775 (+) 315 WP_029973875.1 competence type IV pilus minor pilin ComGE Machinery gene
  C0W57_RS04255 (C0W57_04255) comGF 753720..754184 (+) 465 WP_233717055.1 competence type IV pilus minor pilin ComGF -
  C0W57_RS04260 (C0W57_04260) comGG 754185..754562 (+) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  C0W57_RS04265 (C0W57_04265) - 754619..754798 (+) 180 WP_003153093.1 YqzE family protein -
  C0W57_RS04270 (C0W57_04270) - 754839..755168 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  C0W57_RS04275 (C0W57_04275) tapA 755427..756098 (+) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  C0W57_RS04280 (C0W57_04280) sipW 756070..756654 (+) 585 WP_012117977.1 signal peptidase I SipW -
  C0W57_RS04285 (C0W57_04285) tasA 756719..757504 (+) 786 WP_007408329.1 biofilm matrix protein TasA -
  C0W57_RS04290 (C0W57_04290) sinR 757552..757887 (-) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  C0W57_RS04295 (C0W57_04295) sinI 757921..758094 (-) 174 WP_014418369.1 anti-repressor SinI Regulator

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16254.76 Da        Isoelectric Point: 10.1850

>NTDB_id=264608 C0W57_RS04245 WP_007612572.1 753040..753477(+) (comGD) [Bacillus velezensis strain 10075]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=264608 C0W57_RS04245 WP_007612572.1 753040..753477(+) (comGD) [Bacillus velezensis strain 10075]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCTTTTGATTCGCTTCACATTACGCTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATACAATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

55.479

100

0.559

Multiple sequence alignment