Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   C1S74_RS12630 Genome accession   NZ_CP025794
Coordinates   2554589..2555182 (+) Length   197 a.a.
NCBI ID   WP_045399104.1    Uniprot ID   -
Organism   Vibrio hyugaensis strain 090810a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549589..2560182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S74_RS12615 - 2550288..2552852 (-) 2565 WP_045399114.1 PBP1A family penicillin-binding protein -
  C1S74_RS12620 pilM 2553030..2554046 (+) 1017 WP_045399109.1 type IV pilus assembly protein PilM Machinery gene
  C1S74_RS12625 pilN 2554030..2554596 (+) 567 WP_045399107.1 PilN domain-containing protein Machinery gene
  C1S74_RS12630 pilO 2554589..2555182 (+) 594 WP_045399104.1 type 4a pilus biogenesis protein PilO Machinery gene
  C1S74_RS12635 pilP 2555172..2555687 (+) 516 WP_045399101.1 pilus assembly protein PilP Machinery gene
  C1S74_RS12640 pilQ 2555722..2557473 (+) 1752 WP_045399099.1 type IV pilus secretin PilQ family protein Machinery gene
  C1S74_RS12645 aroK 2557662..2558180 (+) 519 WP_038865492.1 shikimate kinase AroK -
  C1S74_RS12650 aroB 2558212..2559312 (+) 1101 WP_045399097.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23115.70 Da        Isoelectric Point: 4.9505

>NTDB_id=263840 C1S74_RS12630 WP_045399104.1 2554589..2555182(+) (pilO) [Vibrio hyugaensis strain 090810a]
MVNWQDLDIDEIAEWPVLPQLVVILLMMLLIQGFGYWFYLKPEEERLNTLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDAK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=263840 C1S74_RS12630 WP_045399104.1 2554589..2555182(+) (pilO) [Vibrio hyugaensis strain 090810a]
GTGGTTAATTGGCAAGATCTCGATATAGATGAAATTGCTGAGTGGCCAGTGCTGCCTCAGTTGGTTGTCATCTTATTAAT
GATGCTTTTGATCCAAGGCTTTGGCTATTGGTTCTATCTAAAGCCGGAGGAGGAGCGCTTAAATACGCTGATCCAGGAAG
AGCAAACCTTAAAAGTCGCTTTGCGTATCAAGGCCAACAAAGTGGCGGCGTTACCGCAATTGCAATCTCAACTTGATGAG
CTAACCACGCGCTACGAGTTGTTGCTGCAGCAACTGCCTGCGCAAAAAGAACTAGCGACCATGTTGGCTTCGGTCAACGA
ATTGGGGATCGACAATAAGCTGACGTTTACTCGAATTGACTGGGGCGAGAAGCAATCTGAACACTTCCTTTACCGTTTAC
CACTCAACATCGAGTTAACAGGAGATTTCCACGACATCGGCCGTTTCTCTCAGGCTATCGCGACGTTACCTCGCATCATC
ACATTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAAAGTGAAACGTTGCACTTCCGTGTTAGGGCGAATACTTACCA
GTTCAAACCAGAGGAAAAGAAAGATGCGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

93.684

96.447

0.904
  pilO Vibrio cholerae strain A1552

73.298

96.954

0.711

Multiple sequence alignment