Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C1S74_RS12620 Genome accession   NZ_CP025794
Coordinates   2553030..2554046 (+) Length   338 a.a.
NCBI ID   WP_045399109.1    Uniprot ID   -
Organism   Vibrio hyugaensis strain 090810a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2548030..2559046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S74_RS12605 - 2548408..2549136 (-) 729 WP_045399116.1 redoxin family protein -
  C1S74_RS12610 oxyR 2549310..2550218 (+) 909 WP_038865506.1 DNA-binding transcriptional regulator OxyR -
  C1S74_RS12615 - 2550288..2552852 (-) 2565 WP_045399114.1 PBP1A family penicillin-binding protein -
  C1S74_RS12620 pilM 2553030..2554046 (+) 1017 WP_045399109.1 type IV pilus assembly protein PilM Machinery gene
  C1S74_RS12625 pilN 2554030..2554596 (+) 567 WP_045399107.1 PilN domain-containing protein Machinery gene
  C1S74_RS12630 pilO 2554589..2555182 (+) 594 WP_045399104.1 type 4a pilus biogenesis protein PilO Machinery gene
  C1S74_RS12635 pilP 2555172..2555687 (+) 516 WP_045399101.1 pilus assembly protein PilP Machinery gene
  C1S74_RS12640 pilQ 2555722..2557473 (+) 1752 WP_045399099.1 type IV pilus secretin PilQ family protein Machinery gene
  C1S74_RS12645 aroK 2557662..2558180 (+) 519 WP_038865492.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37826.17 Da        Isoelectric Point: 6.3483

>NTDB_id=263838 C1S74_RS12620 WP_045399109.1 2553030..2554046(+) (pilM) [Vibrio hyugaensis strain 090810a]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKDLPFRANRVAISVPDNA
VISKKLQIEQSLEESEKEFAVVQAFSHQSPFPVEELSLDFVLVESEEQMRGTESYQVFATRKDVVETRVEALQKSGLKTV
LVDVHSQSLGHIWKLTTERYPDKSKYCLLDIGVLNSSFTMFTEQGELFHKEFVSGTRAASGLLHDNPLSDDAQVMTEQFN
RQVVDRVKRQMQLYTSINGSQDIKGIWLSGEGASTPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTTAA
GLAVRGIQWLGGNHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=263838 C1S74_RS12620 WP_045399109.1 2553030..2554046(+) (pilM) [Vibrio hyugaensis strain 090810a]
ATGGATAAGCTATTAGTTACGGGTATAGATATCGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTATGCCTTACTGGGTTACAAAGAAATAATACTTAAAGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTAAAAGAGATGAAAAAAGACCTCCCTTTTAGGGCTAATCGAGTCGCGATTTCGGTACCTGACAATGCG
GTGATCAGCAAAAAACTGCAAATCGAACAAAGTTTAGAGGAAAGTGAAAAAGAGTTTGCTGTCGTACAGGCTTTTTCTCA
TCAATCGCCGTTCCCTGTAGAAGAGCTTAGTCTTGATTTTGTATTAGTGGAATCTGAAGAGCAAATGCGTGGGACAGAAA
GCTATCAAGTTTTTGCAACACGTAAAGACGTGGTGGAAACCCGCGTAGAAGCATTGCAAAAATCTGGTCTAAAAACCGTG
CTGGTGGATGTGCATTCCCAAAGTCTTGGGCATATCTGGAAGTTAACCACTGAACGTTATCCTGACAAAAGCAAATACTG
CCTACTGGACATCGGGGTATTAAATAGCTCGTTCACTATGTTTACCGAGCAAGGCGAGTTATTCCATAAGGAGTTTGTGT
CTGGCACTCGTGCCGCTAGCGGCCTTTTGCACGACAACCCGCTTTCTGATGATGCTCAAGTCATGACGGAACAATTCAAC
CGTCAAGTTGTGGATCGTGTGAAACGTCAAATGCAGCTCTATACCTCTATTAATGGTTCTCAAGATATTAAAGGTATTTG
GCTCTCCGGTGAGGGCGCATCCACTCCGATGTTAGCCGAGGAGCTTTCCAGCCAACTGGCTCTAGAATGTGAGTTGCTTA
ACCCACTGGGCTTATTTGAAATGAAAGTTTCTAAGCGCAAGCGCCGAGCCGCGGATTGGCAGCACTTTACCACTGCTGCG
GGTTTAGCTGTACGTGGTATCCAGTGGTTGGGAGGCAATCATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

88.166

100

0.882
  pilM Vibrio cholerae strain A1552

49.415

100

0.5

Multiple sequence alignment