Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C1H71_RS03150 Genome accession   NZ_CP025781
Coordinates   746659..747825 (-) Length   388 a.a.
NCBI ID   WP_130105274.1    Uniprot ID   A0A7G3G5X2
Organism   Iodobacter fluviatilis strain PCH194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 741659..752825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1H71_RS03125 (C1H71_03160) - 742355..742537 (-) 183 WP_130105269.1 Trm112 family protein -
  C1H71_RS03130 (C1H71_03165) lpxK 742696..743673 (-) 978 WP_130105270.1 tetraacyldisaccharide 4'-kinase -
  C1H71_RS03135 (C1H71_03170) - 743706..744128 (-) 423 WP_130105271.1 biopolymer transporter ExbD -
  C1H71_RS03140 (C1H71_03175) - 744131..744739 (-) 609 WP_130105272.1 MotA/TolQ/ExbB proton channel family protein -
  C1H71_RS03145 (C1H71_03180) xseA 745232..746587 (+) 1356 WP_130105273.1 exodeoxyribonuclease VII large subunit -
  C1H71_RS03150 (C1H71_03185) pilU 746659..747825 (-) 1167 WP_130105274.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C1H71_RS03155 (C1H71_03190) - 748088..749017 (+) 930 WP_130105275.1 Nudix family hydrolase -
  C1H71_RS03160 (C1H71_03195) - 749014..749451 (+) 438 WP_130105276.1 hypothetical protein -
  C1H71_RS03165 (C1H71_03200) - 749452..749955 (+) 504 WP_130105277.1 M48 family metallopeptidase -
  C1H71_RS03170 (C1H71_03205) gspG 750225..750641 (+) 417 WP_130105278.1 type II secretion system major pseudopilin GspG -
  C1H71_RS03175 (C1H71_03210) - 750731..751903 (+) 1173 WP_130105279.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43455.94 Da        Isoelectric Point: 6.1546

>NTDB_id=263734 C1H71_RS03150 WP_130105274.1 746659..747825(-) (pilU) [Iodobacter fluviatilis strain PCH194]
MTPEQANKTINDLLRMMLQHHASDLFIADDFPPAMKINGKLTPVGNQKLNGVVSKALAYSVMRVDQQEEFEREYECNFAI
NPEGIGRFRVNVFMQQEKVGMVLRVITSKIPDFDELKLPPVLKEVVLAKRGLVLLVGGTGSGKSTTMAAMIDYRNQNTHG
HIITIEDPIEYVHKSKNCLVTHREVGRDTKHWFAALKNTLRQAPDVILIGEIRDRDTMEYALSFAETGHLCMGTLHANSA
NQALERIVNFFPEERHPQIYMDLSLNMRGMISQRLIPTHDGKGRVAAIEVLLNSPLVADMIFKGDVGGVKTAMLKSEELG
MQTFDSALFHLYEDGRISLEEALKNADSINELRLRVKLKSKDSKSVGHDELGHLSLVAEAEPEEEEAP

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=263734 C1H71_RS03150 WP_130105274.1 746659..747825(-) (pilU) [Iodobacter fluviatilis strain PCH194]
ATGACGCCAGAACAAGCCAATAAAACGATCAATGATTTGCTGCGCATGATGCTGCAGCATCATGCGTCTGATTTATTTAT
TGCCGATGATTTTCCGCCTGCAATGAAAATTAATGGCAAGCTCACGCCGGTTGGCAATCAAAAGCTCAATGGAGTGGTCA
GCAAGGCATTGGCGTATTCGGTAATGCGGGTTGATCAACAGGAAGAATTTGAGCGCGAATATGAATGCAATTTTGCGATT
AATCCAGAAGGCATCGGCCGTTTTCGGGTTAATGTGTTTATGCAGCAAGAAAAAGTCGGCATGGTGCTGCGGGTTATTAC
CAGCAAGATCCCCGATTTTGATGAGCTAAAACTCCCCCCTGTTTTAAAAGAAGTGGTGCTGGCCAAACGTGGCTTGGTGC
TTTTGGTGGGCGGAACAGGATCGGGTAAATCAACCACAATGGCGGCCATGATTGATTACCGTAATCAAAACACCCACGGC
CATATCATCACCATTGAAGACCCGATTGAATACGTGCATAAAAGTAAAAACTGCTTGGTGACGCATCGCGAAGTAGGCCG
CGACACTAAGCATTGGTTTGCGGCACTTAAAAACACCCTAAGACAAGCACCCGATGTGATTTTAATTGGTGAAATTCGTG
ATCGCGATACCATGGAATACGCTTTGTCTTTTGCTGAAACAGGCCATTTATGCATGGGTACTTTGCATGCCAATAGTGCG
AATCAGGCTTTGGAGCGCATTGTTAATTTCTTCCCCGAAGAGCGTCATCCACAAATTTATATGGATTTATCCTTAAATAT
GCGTGGGATGATTTCACAGCGCTTAATTCCTACCCATGATGGAAAAGGGCGTGTGGCTGCGATTGAGGTTTTGCTTAATT
CGCCTTTAGTGGCGGATATGATTTTTAAAGGCGATGTGGGCGGGGTTAAAACTGCCATGCTTAAATCAGAAGAGCTAGGG
ATGCAAACCTTTGATTCTGCACTCTTTCATTTATATGAAGATGGGCGAATTAGCCTAGAAGAAGCACTTAAAAACGCTGA
CTCGATTAATGAATTACGTTTGCGGGTTAAATTAAAAAGCAAAGACAGCAAAAGCGTTGGTCACGATGAGTTAGGCCATT
TAAGCTTGGTGGCCGAAGCCGAGCCAGAGGAGGAGGAAGCACCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G3G5X2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.166

89.948

0.523
  pilU Acinetobacter baylyi ADP1

56.077

93.299

0.523
  pilU Vibrio cholerae strain A1552

54.19

92.268

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.027

86.856

0.374
  pilT Pseudomonas aeruginosa PAK

43.027

86.856

0.374

Multiple sequence alignment