Detailed information NTG0026366

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: APL_RS01470
• Genome accession: NC_009053
• Coordinates: 313659..314663 (+)
• Length: 334 a.a.
• NCBI ID: WP_005600359.1
• Uniprot ID: A3MZ06
• Organism: Actinobacillus pleuropneumoniae serovar 5b str. L20
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 308659..319663

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
APL_RS01450 (APL_0280)	potA	310158..311273 (+)	1116	WP_005600350.1	spermidine/putrescine ABC transporter ATP-binding protein PotA	-
APL_RS01455 (APL_0281)	potB	311260..312126 (+)	867	WP_005603539.1	spermidine/putrescine ABC transporter permease PotB	-
APL_RS01460 (APL_0282)	potC	312126..312893 (+)	768	WP_043880758.1	spermidine/putrescine ABC transporter permease PotC	-
APL_RS01465 (APL_0283)	ruvA	313035..313640 (+)	606	WP_005600357.1	Holliday junction branch migration protein RuvA	Machinery gene
APL_RS01470 (APL_0284)	ruvB	313659..314663 (+)	1005	WP_005600359.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
APL_RS01475 (APL_0285)	ubiG	314720..315424 (-)	705	WP_005611396.1	bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG	-
APL_RS01480 (APL_0286)	gyrA	315604..318294 (+)	2691	WP_009874680.1	DNA topoisomerase (ATP-hydrolyzing) subunit A	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 36929.33 Da
• Isoelectric Point: 4.7294

>NTDB_id=26366 APL_RS01470 WP_005600359.1 313659..314663(+) (ruvB) [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVREQMEIFIKAAKLRDEALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTSIVKRSADCLNLNLSPDGAYEVARRSRGTPRIANRLLRRVRDYADVRNNG
VITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSRTYAHLGIAKLD

Nucleotide

• Length: 1005 bp

>NTDB_id=26366 APL_RS01470 WP_005600359.1 313659..314663(+) (ruvB) [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGATTGAAGCAGATAGAATAATTAGTGCCTCGCCGAAGCGTGAGGAAGAAGTCATCGATCGTGCGATTCGTCCTAAATT
ATTAGCGGATTATGTCGGTCAGCCGTCTGTACGAGAGCAGATGGAGATCTTTATTAAAGCGGCAAAATTGCGTGATGAGG
CGCTAGATCACTTATTAATTTTTGGTCCGCCGGGATTGGGTAAAACCACCCTAGCTAATATTGTTGCCAACGAAATGGGC
GTGAATATTCGCACAACTTCCGGACCTGTGTTAGAGAAAGCGGGCGATCTAGCGGCAATGCTGACTAATTTAGAGCCTTA
TGATGTGTTATTTATTGATGAGATCCACCGCTTATCACCGGCAATTGAAGAAGTGCTTTATCCGGCGATGGAAGATTATC
AGTTGGATATTATGATTGGCGAAGGGCCGGCGGCACGTTCAATTAAACTAGATTTACCGCCGTTTACCTTAGTGGGAGCA
ACCACCCGTGCCGGTTCACTGACTTCTCCGTTACGCGACCGTTTTGGAATTGTACAGCGTTTAGAGTTTTACTCGGTAGA
TGATTTAACCTCGATCGTAAAACGTAGTGCCGATTGCTTAAACTTAAATTTATCGCCGGACGGTGCTTATGAAGTGGCTC
GCCGTTCACGTGGCACCCCTCGAATTGCTAACCGTTTATTACGCCGTGTGCGAGATTATGCCGATGTGCGTAATAACGGT
GTGATTACCTCTGATATTGCTAAACAAGCGTTGGCGATGCTTGATGTAGATTCCGAAGGCTTTGACTTTATGGACATCAA
ATTATTGCAAGCGATTGTAGAACGCTTTGACGGAGGCCCGGTCGGCTTAGATAATTTAGCTGCAGCGATAGGTGAAGAGC
GAGATACGATTGAAGACGTACTTGAACCTTATTTGATTCAGCAGGGTTTTTTACAGCGAACGCCACGAGGGCGTATTGCT
ACTAGCCGAACTTATGCACATTTAGGCATTGCAAAATTAGATTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

3D structure

• AlphaFold DB: A3MZ06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	62.346	97.006	0.605
ruvB	Streptococcus pneumoniae TIGR4	59.259	97.006	0.575
ruvB	Streptococcus pneumoniae R6	59.259	97.006	0.575
ruvB	Streptococcus pneumoniae D39	59.259	97.006	0.575
ruvB	Synechocystis sp. PCC 6803	54.969	96.407	0.53
ruvB	Helicobacter pylori 26695	54.952	93.713	0.515