Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H16_RS16220 Genome accession   NC_008313
Coordinates   3515884..3517608 (+) Length   574 a.a.
NCBI ID   WP_011616081.1    Uniprot ID   -
Organism   Cupriavidus necator H16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3510884..3522608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H16_RS16190 (H16_A3250) obgE 3511554..3512651 (-) 1098 WP_010814746.1 GTPase ObgE -
  H16_RS16195 (H16_A3251) rpmA 3512785..3513045 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  H16_RS16200 (H16_A3252) rplU 3513085..3513396 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  H16_RS16205 (H16_A3253) ispB 3513927..3514856 (+) 930 WP_010814748.1 octaprenyl diphosphate synthase -
  H16_RS16215 - 3515140..3515460 (-) 321 WP_231881363.1 helix-turn-helix domain-containing protein -
  H16_RS16220 (H16_A3255) pilF 3515884..3517608 (+) 1725 WP_011616081.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H16_RS16225 (H16_A3256) - 3517673..3518944 (+) 1272 WP_011616082.1 type II secretion system F family protein -
  H16_RS16230 (H16_A3257) pilD 3518948..3519862 (+) 915 WP_011616083.1 prepilin peptidase Machinery gene
  H16_RS16235 (H16_A3258) coaE 3519926..3520552 (+) 627 WP_010814753.1 dephospho-CoA kinase -
  H16_RS16240 (H16_A3259) zapD 3520762..3521520 (+) 759 WP_011616084.1 cell division protein ZapD -
  H16_RS16245 - 3521538..3521726 (+) 189 WP_010814755.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63385.00 Da        Isoelectric Point: 7.0571

>NTDB_id=26245 H16_RS16220 WP_011616081.1 3515884..3517608(+) (pilF) [Cupriavidus necator H16]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLAQYNLNKVPPALAGNREF
HAHRLLPLGRRENRLVLAMSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKHVRSAGEALGTLKNISPVQAERKMIEYDP
VAAAGNQRNRTTADNIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDKDAKETVERAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAAFNIASSVLMITAQRLARRLCTCKREGEIPREALL
EAGFREQDLDGSWQAYHPVGCERCNGTGYKGRCGIYQVMPITEAMQEIILTHGTALQIAEQARKDGVLSLREAGLLKVRQ
GVTSLEEVLATTNT

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=26245 H16_RS16220 WP_011616081.1 3515884..3517608(+) (pilF) [Cupriavidus necator H16]
ATGACACTCGGTCTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCGCGTGA
AAAGCAGTCGCAGCTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCGCTGTTTGCCGCGGACA
AATACCAGCTGCCGCTGCTGGACCTGGCCCAGTACAACCTGAACAAGGTGCCGCCGGCACTGGCCGGCAACCGTGAATTC
CACGCGCACCGGCTGCTGCCGCTGGGCCGGCGCGAGAACCGGCTGGTGCTGGCGATGTCCGACCCGTCCAACCAGGCCGG
GCTGGATGCGATCAAGGAAAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCACGTGC
GCTCCGCCGGCGAGGCCCTGGGCACGCTGAAGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGATCCG
GTGGCCGCGGCCGGCAACCAGCGCAACCGCACCACCGCCGACAATATCGACGACGCCCCGGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAGGCCTTCCATCGCGGCGCGTCCGACCTGCACTTCGAGCCGTTCGAGACTTTCTACCGCGTCCGCTTCC
GCGTGGACGGGGTGCTGCAGGAGGTCGCGCGCCCACCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTG
TCGCGCCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATTGCCCTGCCCAAGGACAAGGA
CGCCAAGGAGACGGTCGAGCGGGCAGTGGATTTCCGCGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGC
GGATCCTGGAATCTTCGTCCGACAAGCTCGACATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGGCGCTGCTGCTGGAC
GTGATCAAGCGCCCCTATGGCATGGTGCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACCTGCTGAACCAGGGCGACATCAATATCTCCACCGCGGAAGACCCGGCCGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGCCTGACCTTTGCCGCGGCCCTGCGTTCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGTTGTCGACGCT
GCACACCAACGACGCGCCGACCACGCTGACGCGGTTGATGAACATGGGCGTGGCGGCCTTCAATATTGCCTCGAGCGTAC
TGATGATCACCGCGCAGCGGCTGGCGCGGCGGCTGTGCACCTGCAAGCGCGAGGGCGAGATCCCGCGCGAGGCGCTGCTG
GAGGCGGGCTTCCGCGAACAAGACCTGGACGGCAGCTGGCAGGCCTACCACCCGGTCGGCTGCGAGCGCTGCAACGGCAC
CGGCTACAAGGGCCGCTGCGGCATCTACCAGGTCATGCCGATCACCGAGGCCATGCAGGAGATCATCCTGACGCATGGAA
CGGCATTGCAGATCGCCGAGCAGGCGCGCAAGGACGGCGTGCTATCGTTGCGCGAAGCGGGGCTGCTGAAGGTGAGGCAG
GGCGTCACGTCACTCGAAGAAGTGCTGGCGACTACGAATACTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

51.813

100

0.523
  pilB Acinetobacter baumannii D1279779

52.281

99.303

0.519
  pilB Acinetobacter baylyi ADP1

52.252

96.69

0.505
  pilB Legionella pneumophila strain ERS1305867

49.909

95.993

0.479
  pilB Vibrio cholerae strain A1552

45.343

99.129

0.449
  pilB Vibrio campbellii strain DS40M4

46.111

94.077

0.434
  pilB Vibrio parahaemolyticus RIMD 2210633

46.111

94.077

0.434
  pilF Thermus thermophilus HB27

39.168

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.273

95.819

0.376

Multiple sequence alignment