Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   SPSF3K_RS01940 Genome accession   NZ_CP025420
Coordinates   407483..408538 (+) Length   351 a.a.
NCBI ID   WP_152414240.1    Uniprot ID   -
Organism   Streptococcus parauberis strain SPOF3K     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 402483..413538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPSF3K_RS01930 (SPSF3K_00399) - 406202..406576 (+) 375 WP_003108753.1 DUF1033 family protein -
  SPSF3K_RS01935 (SPSF3K_00400) comYA 406630..407571 (+) 942 WP_003108751.1 competence type IV pilus ATPase ComGA Machinery gene
  SPSF3K_RS01940 (SPSF3K_00401) comYB 407483..408538 (+) 1056 WP_152414240.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPSF3K_RS01945 (SPSF3K_00402) comYC 408535..408861 (+) 327 WP_004347181.1 competence type IV pilus major pilin ComGC Machinery gene
  SPSF3K_RS01950 (SPSF3K_00403) comGD 408851..409258 (+) 408 WP_243619590.1 competence type IV pilus minor pilin ComGD -
  SPSF3K_RS01955 (SPSF3K_00404) comGE 409230..409529 (+) 300 WP_003108643.1 competence type IV pilus minor pilin ComGE -
  SPSF3K_RS01960 (SPSF3K_00405) comGF 409507..409944 (+) 438 WP_003102915.1 competence type IV pilus minor pilin ComGF -
  SPSF3K_RS01965 (SPSF3K_00406) comGG 409922..410419 (+) 498 WP_003108640.1 competence type IV pilus minor pilin ComGG -
  SPSF3K_RS01970 (SPSF3K_00407) comYH 410526..411482 (+) 957 WP_003103712.1 class I SAM-dependent methyltransferase Machinery gene
  SPSF3K_RS01975 (SPSF3K_00408) - 411538..412731 (+) 1194 WP_003108638.1 acetate kinase -
  SPSF3K_RS01980 (SPSF3K_00409) - 412842..413054 (+) 213 WP_003108636.1 helix-turn-helix transcriptional regulator -
  SPSF3K_RS01985 (SPSF3K_00410) - 413047..413499 (+) 453 WP_003108634.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 40401.42 Da        Isoelectric Point: 9.8078

>NTDB_id=261153 SPSF3K_RS01940 WP_152414240.1 407483..408538(+) (comYB) [Streptococcus parauberis strain SPOF3K]
MKNGIKLLISWLQKDISLKKKGKKKRLSSKKQYQLIQLLHNLQASGFNLVEMVSFLKKSKLISEEQVKIMEFHMLNGSSL
SKIMLDLGYPDSIITQLSLAELHGNILGSLENIETYLLQVNHIKKKTFEVVMYPLVLLFFLTTILLGLRQYLLPSLNEEG
PLMFLLTYFPHLLIILIVIILFVSIVVIYKWRHSSPLKLISQISRLPLIGYVIQLYMTAFYAREWGNLLDQGLELSTILS
VMSKEKSLLVREIGIEMNSAFVEGIPFEQKVLDYPFFKNELSLIISYGQVKSKLGKELEIYSQNTWESYFRFLIQLTQLI
QPIVFLAVALIIVMIYAAMLLPIYQNMGGNL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=261153 SPSF3K_RS01940 WP_152414240.1 407483..408538(+) (comYB) [Streptococcus parauberis strain SPOF3K]
ATGAAAAATGGAATCAAGCTATTGATCAGTTGGTTGCAAAAGGACATTTCCCTGAAGAAAAAGGGGAAAAAGAAAAGATT
ATCGAGTAAAAAACAATATCAACTTATCCAATTGTTACATAATCTCCAAGCAAGCGGATTTAATTTAGTTGAAATGGTAT
CTTTTCTGAAGAAAAGTAAATTAATATCTGAAGAGCAGGTTAAAATTATGGAATTTCACATGCTCAATGGCAGTAGTTTA
TCAAAAATCATGCTTGATTTAGGTTACCCAGATAGTATTATAACTCAATTGAGTTTAGCAGAATTACATGGGAATATTTT
GGGGAGTCTTGAAAATATAGAAACATACCTTTTGCAAGTGAATCATATTAAAAAGAAAACTTTCGAAGTGGTAATGTATC
CACTTGTATTACTATTTTTTCTAACAACTATACTACTTGGTTTGAGACAATATTTATTGCCTTCGCTTAATGAGGAAGGT
CCACTTATGTTCCTACTAACCTATTTTCCTCACCTTTTAATAATCCTAATTGTGATAATCCTCTTTGTCTCTATAGTAGT
TATTTATAAATGGCGACACAGTTCACCACTCAAGCTAATTAGCCAAATTAGTCGATTGCCTTTAATAGGATACGTCATCC
AGTTATATATGACAGCCTTCTATGCTCGAGAATGGGGAAATTTATTAGATCAGGGGCTTGAATTAAGTACGATTTTGTCT
GTTATGAGTAAGGAAAAATCACTTTTGGTACGGGAAATAGGTATAGAAATGAATAGTGCTTTTGTAGAGGGGATTCCGTT
TGAGCAAAAGGTACTAGATTATCCATTTTTTAAAAATGAATTGAGTTTAATTATTTCATATGGACAAGTTAAATCAAAAT
TAGGGAAGGAGTTAGAAATATATTCTCAAAATACATGGGAGTCTTACTTCAGGTTCCTCATACAACTTACTCAGCTGATT
CAACCAATTGTATTTTTAGCAGTTGCTCTAATTATTGTCATGATTTATGCAGCAATGCTATTGCCAATTTATCAAAATAT
GGGAGGAAATTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(36-154)

Type II secretion system (T2SS), protein F (T2SSF)

(222-342)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

51.304

98.291

0.504
  comYB Streptococcus mutans UA159

51.304

98.291

0.504
  comYB Streptococcus gordonii str. Challis substr. CH1

46.765

96.866

0.453
  comGB/cglB Streptococcus mitis NCTC 12261

46.471

96.866

0.45
  comGB/cglB Streptococcus mitis SK321

45.104

96.011

0.433
  comGB/cglB Streptococcus pneumoniae Rx1

44.807

96.011

0.43
  comGB/cglB Streptococcus pneumoniae D39

44.807

96.011

0.43
  comGB/cglB Streptococcus pneumoniae R6

44.807

96.011

0.43
  comGB/cglB Streptococcus pneumoniae TIGR4

44.807

96.011

0.43
  comGB Lactococcus lactis subsp. cremoris KW2

42.229

97.151

0.41

Multiple sequence alignment