Detailed information NTG0259985

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CXP43_RS13565
• Genome accession: NZ_CP025308
• Coordinates: 2724286..2724900 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain Lzh-a42
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2719286..2729900

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CXP43_RS13540 (CXP43_13540)	rpsT	2719589..2719855 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CXP43_RS13545 (CXP43_13545)	holA	2719872..2720913 (-)	1042	Protein_2621	DNA polymerase III subunit delta	-
CXP43_RS21500	-	2720953..2721104 (-)	152	Protein_2622	hypothetical protein	-
CXP43_RS13550 (CXP43_13550)	-	2721145..2721279 (+)	135	WP_003152870.1	YqzM family protein	-
CXP43_RS13555 (CXP43_13555)	comEC	2721298..2723649 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CXP43_RS13560 (CXP43_13560)	-	2723650..2724219 (-)	570	WP_003152868.1	ComE operon protein 2	-
CXP43_RS13565 (CXP43_13565)	comEA	2724286..2724900 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
CXP43_RS13570 (CXP43_13570)	comER	2724959..2725780 (+)	822	WP_014418420.1	late competence protein ComER	-
CXP43_RS13575 (CXP43_13575)	-	2725849..2726586 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
CXP43_RS13580 (CXP43_13580)	rsfS	2726583..2726939 (-)	357	WP_007408260.1	ribosome silencing factor	-
CXP43_RS13585 (CXP43_13585)	yqeK	2726957..2727517 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CXP43_RS13590 (CXP43_13590)	-	2727507..2728076 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CXP43_RS13595 (CXP43_13595)	yhbY	2728087..2728377 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CXP43_RS13600 (CXP43_13600)	aroE	2728371..2729213 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=259985 CXP43_RS13565 WP_014418419.1 2724286..2724900(-) (comEA) [Bacillus velezensis strain Lzh-a42]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=259985 CXP43_RS13565 WP_014418419.1 2724286..2724900(-) (comEA) [Bacillus velezensis strain Lzh-a42]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363