Detailed information NTG0258689

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CXB48_RS20325
• Genome accession: NZ_CP025149
• Coordinates: 4254003..4255148 (-)
• Length: 381 a.a.
• NCBI ID: WP_095460263.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain SGAir0442
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4249003..4260148

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CXB48_RS20300 (CXB48_20240)	-	4249079..4250095 (+)	1017	WP_011914964.1	aspartate carbamoyltransferase catalytic subunit	-
CXB48_RS20305 (CXB48_20245)	-	4250095..4251369 (+)	1275	WP_011914965.1	dihydroorotase	-
CXB48_RS20310 (CXB48_20250)	-	4251427..4251822 (-)	396	WP_011914966.1	NINE protein	-
CXB48_RS20315 (CXB48_20255)	-	4251958..4253172 (+)	1215	WP_013984321.1	putative DNA modification/repair radical SAM protein	-
CXB48_RS20320 (CXB48_20260)	-	4253172..4253996 (+)	825	WP_095460262.1	TIGR03915 family putative DNA repair protein	-
CXB48_RS20325 (CXB48_20265)	pilU	4254003..4255148 (-)	1146	WP_095460263.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CXB48_RS20330 (CXB48_20270)	pilT	4255208..4256242 (-)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
CXB48_RS20335 (CXB48_20275)	-	4256309..4256998 (+)	690	WP_108242416.1	YggS family pyridoxal phosphate-dependent enzyme	-
CXB48_RS20340 (CXB48_20280)	proC	4257009..4257833 (+)	825	WP_108242417.1	pyrroline-5-carboxylate reductase	-
CXB48_RS20345 (CXB48_20285)	-	4257843..4258433 (+)	591	WP_011914973.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42552.85 Da
• Isoelectric Point: 6.8856

>NTDB_id=258689 CXB48_RS20325 WP_095460263.1 4254003..4255148(-) (pilU) [Stutzerimonas stutzeri strain SGAir0442]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=258689 CXB48_RS20325 WP_095460263.1 4254003..4255148(-) (pilU) [Stutzerimonas stutzeri strain SGAir0442]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGTGCCCGCGGCATCGGTCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATTCATTCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACAATGGACTATG
CCGTAGCGTTCGCCGAAACCGGACACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCACCGGATCGGCACAACCAGGTCTGGATGGATCTATCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCGCTGTTGCACGCCGACTCGGCCAACGACCTGCGGCT
GTTAATCAAGCTGGGTTCGGAAACCGACGGCGAGCACCTGACCAGCGTATCCCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.276	100	0.953
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391