Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CXB48_RS20330 Genome accession   NZ_CP025149
Coordinates   4255208..4256242 (-) Length   344 a.a.
NCBI ID   WP_011914970.1    Uniprot ID   A0A0H3YZL0
Organism   Stutzerimonas stutzeri strain SGAir0442     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4250208..4261242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXB48_RS20310 (CXB48_20250) - 4251427..4251822 (-) 396 WP_011914966.1 NINE protein -
  CXB48_RS20315 (CXB48_20255) - 4251958..4253172 (+) 1215 WP_013984321.1 putative DNA modification/repair radical SAM protein -
  CXB48_RS20320 (CXB48_20260) - 4253172..4253996 (+) 825 WP_095460262.1 TIGR03915 family putative DNA repair protein -
  CXB48_RS20325 (CXB48_20265) pilU 4254003..4255148 (-) 1146 WP_095460263.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CXB48_RS20330 (CXB48_20270) pilT 4255208..4256242 (-) 1035 WP_011914970.1 type IV pilus twitching motility protein PilT Machinery gene
  CXB48_RS20335 (CXB48_20275) - 4256309..4256998 (+) 690 WP_108242416.1 YggS family pyridoxal phosphate-dependent enzyme -
  CXB48_RS20340 (CXB48_20280) proC 4257009..4257833 (+) 825 WP_108242417.1 pyrroline-5-carboxylate reductase -
  CXB48_RS20345 (CXB48_20285) - 4257843..4258433 (+) 591 WP_011914973.1 YggT family protein -
  CXB48_RS20350 (CXB48_20290) - 4258703..4260670 (+) 1968 WP_013984327.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38073.88 Da        Isoelectric Point: 6.8359

>NTDB_id=258690 CXB48_RS20330 WP_011914970.1 4255208..4256242(-) (pilT) [Stutzerimonas stutzeri strain SGAir0442]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLNMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLLAKGLISRDSAREKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=258690 CXB48_RS20330 WP_011914970.1 4255208..4256242(-) (pilT) [Stutzerimonas stutzeri strain SGAir0442]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGTGACGTGCGGCGCATCAATCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAAACCGACTTCTCGTTCGAGGTGCCGGGTGTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGACCATGGA
GGACCTCAACATGGGTGAGGTCTTCCGCAAGATCACCGACGTGCCGCGTGGGCTGGTGCTGGTCACCGGGCCCACCGGCT
CGGGTAAGTCGACCACCCTGGCGGCGATGCTCGACTACCTGAACAACACCAAATACCACCACATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTGCACCGCGACACCCTGGGCTTCTCCGA
GGCGCTGCGCTCGGCCTTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTCGTGTTCGGCACGCTGCACACCACCTCCGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAAAAGTCCATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGCGTCGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGTTCGCTCGGCATGCAGACCCTGGACATGTGCCTC
AAGGGGCTGCTGGCCAAGGGCCTGATCAGCCGCGACAGCGCGCGGGAAAAGGCCAAGCAGCCCGAGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3YZL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

97.965

100

0.98
  pilT Pseudomonas aeruginosa PAK

95.058

100

0.951
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter nosocomialis M2

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

83.721

100

0.837
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment