Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   DOU34_RS07540 Genome accession   NZ_CP030097
Coordinates   1481845..1483722 (+) Length   625 a.a.
NCBI ID   WP_007409830.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1476845..1488722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS07525 ymcA 1477896..1478327 (+) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  DOU34_RS07530 cotE 1478580..1479125 (+) 546 WP_003154111.1 outer spore coat protein CotE -
  DOU34_RS07535 hexA 1479244..1481829 (+) 2586 WP_007409829.1 DNA mismatch repair protein MutS Machinery gene
  DOU34_RS07540 hexB 1481845..1483722 (+) 1878 WP_007409830.1 DNA mismatch repair endonuclease MutL Machinery gene
  DOU34_RS07545 - 1483774..1484388 (-) 615 WP_020955840.1 site-specific integrase -
  DOU34_RS07550 - 1484826..1485128 (+) 303 WP_007409833.1 hypothetical protein -
  DOU34_RS07560 - 1486354..1486563 (-) 210 WP_020955841.1 hypothetical protein -
  DOU34_RS07575 - 1487527..1488204 (+) 678 WP_032866035.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 625 a.a.        Molecular weight: 70255.41 Da        Isoelectric Point: 6.0002

>NTDB_id=258551 DOU34_RS07540 WP_007409830.1 1481845..1483722(+) (hexB) [Bacillus amyloliquefaciens strain SH-B74]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDANSTVIEIDIEEAGLSSIRVLDNGEGMETDDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEIKTSIGEGAGTHLILQGGNIISEQKSSSRKGTEIIVTNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHQGKNLLQTNGNGDVRHVLAAIYGTAVAKKMIPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVINGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHELIRDG
IKEVFQKQRLIPSAQLPKKSAPAPAKNEQQFMTFEESAPERKLPEKTPEPSYSPMKLSSVVKEPEPVIEEESLPDEAGHG
VPPKIGMPVTEAHVSDIPEPEEEADSEEIREERVPVMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVGQV
EPEVQDMIVPLTFHYAANEALIIDQHQHELESVGVFLEAFGTNSYIVRCHPAWFPKGEEAELIEEIIQQVLDAKQIDIKK
LREEAAIMMSCKGSIKANRHLRNDEIKALLDDLRRTTDPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1878 bp        

>NTDB_id=258551 DOU34_RS07540 WP_007409830.1 1481845..1483722(+) (hexB) [Bacillus amyloliquefaciens strain SH-B74]
TTGGCAAAAGTCATCCAGCTGTCAGATGAACTCTCGAATAAAATAGCGGCCGGCGAGGTGGTCGAACGGCCTGCCTCAGT
CGTTAAAGAATTGGTGGAAAACGCCATCGACGCAAACAGCACAGTCATTGAAATCGACATAGAGGAAGCGGGGCTTTCGT
CGATTCGGGTGCTCGATAACGGCGAAGGGATGGAAACCGATGATTGCAAGCGGGCTTTCCGCCGCCACGCGACCAGTAAA
ATCAAAGATGAAAACGACCTGTTCCGGGTGAGAACACTCGGTTTCCGCGGAGAAGCTTTGCCAAGTATCGCGTCTGTTTC
TCATCTGGAAATCAAAACGAGCATCGGCGAGGGAGCGGGAACCCATCTCATTCTTCAGGGCGGGAATATCATTTCTGAGC
AGAAATCCTCCAGCAGAAAAGGAACGGAGATTATCGTTACCAACCTGTTTTTCAATACACCCGCCAGATTGAAATATATG
AAAACCGTGCATACGGAGCTCGGCAATATTACGGATGTTGTCAACCGGATCGCTCTTGCGCATCCGGAAGTATCGATCCG
TCTCCGCCATCAGGGGAAAAATCTGCTGCAGACAAACGGTAACGGGGACGTGCGCCATGTGCTGGCCGCAATTTACGGGA
CGGCGGTTGCGAAAAAAATGATACCGCTCCATGTCAGCTCGCTTGATTTTGAAGTCAAGGGCTACATTGCGCTTCCGGAA
ATCACGCGTGCATCCAGAAACTACATGTCATCTGTCATTAACGGAAGATACATTAAGAATTTCCCGCTTGTAAAAGCGGT
GCATGAGGGCTATCACACACTACTGCCGATCGGCCGCCATCCGATTACGTTTATCGAAATTACGATGGACCCGATTTTGG
TCGATGTCAATGTCCATCCCTCGAAGCTTGAAGTCCGCCTCAGCAAAGAGACGGAGCTGCATGAGCTGATCCGTGACGGG
ATAAAGGAAGTGTTTCAAAAGCAGCGGCTTATTCCGAGCGCCCAGCTCCCGAAAAAATCCGCGCCGGCACCGGCCAAAAA
CGAACAGCAATTTATGACATTCGAGGAAAGCGCGCCTGAGCGGAAACTTCCTGAAAAAACGCCGGAGCCGTCTTACTCAC
CGATGAAGCTGAGTTCGGTCGTAAAAGAGCCTGAGCCGGTAATAGAAGAAGAGTCCCTGCCGGATGAAGCGGGTCACGGA
GTGCCGCCGAAGATCGGCATGCCGGTAACGGAAGCGCATGTTTCCGATATTCCGGAGCCTGAAGAGGAGGCTGACTCTGA
AGAAATACGGGAAGAAAGAGTTCCGGTGATGTATCCGATCGGCCAAATGCACGGGACATATATTTTAGCCCAAAACGAAA
ACGGCTTGTATATCATTGACCAGCATGCTGCACAAGAACGGATTAAATACGAATATTTCCGGGAGAAAGTTGGGCAGGTC
GAGCCTGAAGTGCAGGATATGATCGTGCCGCTCACCTTTCATTATGCGGCAAATGAGGCGCTGATCATTGACCAGCATCA
GCATGAGCTTGAAAGCGTCGGAGTATTTTTAGAAGCATTCGGCACGAACAGCTACATCGTCCGCTGCCATCCGGCCTGGT
TTCCAAAAGGGGAAGAAGCTGAACTGATTGAAGAAATCATTCAGCAGGTGCTTGACGCCAAACAGATCGATATTAAAAAA
CTGCGGGAGGAAGCGGCCATTATGATGAGCTGCAAAGGCTCAATCAAAGCGAACCGCCATTTGCGAAACGACGAAATCAA
AGCGCTCTTGGATGACCTGCGCCGAACGACAGACCCGTTCACATGCCCGCACGGCCGGCCGATCATAATTCATCACAGCA
CGTATGAGATGGAGAAGATGTTTAAGCGTGTGATGTAG

Domains

Predicted by InterProScan.

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-325)

MutL C terminal dimerisation domain (MutL_C)

(440-581)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-111)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

46.802

100

0.48

Multiple sequence alignment