Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   CWE29_RS12125 Genome accession   NZ_CP025077
Coordinates   2378791..2380077 (+) Length   428 a.a.
NCBI ID   WP_002325236.1    Uniprot ID   A0A366TVN3
Organism   Enterococcus faecium strain LS170308     
Function   repress competence development (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2341238..2394929 2378791..2380077 within 0


Gene organization within MGE regions


Location: 2341238..2394929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWE29_RS11945 (CWE29_11945) - 2342469..2342675 (-) 207 WP_010706118.1 hypothetical protein -
  CWE29_RS11950 (CWE29_11950) - 2342838..2344475 (-) 1638 WP_100970582.1 recombinase family protein -
  CWE29_RS11955 (CWE29_11955) - 2344479..2345852 (-) 1374 WP_002351308.1 recombinase family protein -
  CWE29_RS11960 (CWE29_11960) - 2345869..2346108 (-) 240 WP_043951862.1 hypothetical protein -
  CWE29_RS11965 (CWE29_11965) - 2346540..2347955 (-) 1416 WP_100970583.1 DNA primase family protein -
  CWE29_RS11970 (CWE29_11970) - 2347948..2348319 (-) 372 WP_043951863.1 hypothetical protein -
  CWE29_RS11975 (CWE29_11975) - 2348879..2350057 (+) 1179 WP_000997695.1 IS256-like element ISEf1 family transposase -
  CWE29_RS14130 - 2350400..2350621 (-) 222 WP_002349778.1 hypothetical protein -
  CWE29_RS11985 (CWE29_11985) - 2351049..2351741 (-) 693 WP_002287844.1 hypothetical protein -
  CWE29_RS11990 (CWE29_11990) - 2352046..2353215 (-) 1170 WP_002351288.1 IS256 family transposase -
  CWE29_RS11995 (CWE29_11995) - 2353286..2353837 (-) 552 WP_002299296.1 PD-(D/E)XK nuclease family protein -
  CWE29_RS12000 (CWE29_12000) - 2354292..2355089 (+) 798 WP_100970584.1 MvaI/BcnI family restriction endonuclease -
  CWE29_RS12005 (CWE29_12005) - 2355107..2355970 (-) 864 WP_002336111.1 DNA methyltransferase -
  CWE29_RS12010 (CWE29_12010) - 2355998..2356894 (-) 897 WP_100970585.1 DNA-methyltransferase -
  CWE29_RS12015 (CWE29_12015) - 2357087..2358724 (-) 1638 WP_100970586.1 recombinase family protein -
  CWE29_RS12020 (CWE29_12020) - 2358728..2360101 (-) 1374 WP_100970587.1 recombinase family protein -
  CWE29_RS12025 (CWE29_12025) - 2360117..2360356 (-) 240 WP_002334089.1 hypothetical protein -
  CWE29_RS12030 (CWE29_12030) - 2360538..2363213 (-) 2676 WP_100970588.1 DUF927 domain-containing protein -
  CWE29_RS12035 (CWE29_12035) - 2363396..2363977 (-) 582 WP_232735619.1 hypothetical protein -
  CWE29_RS12040 (CWE29_12040) - 2364061..2365233 (-) 1173 WP_002323245.1 IS256-like element IS1542 family transposase -
  CWE29_RS12045 (CWE29_12045) - 2365310..2365651 (-) 342 WP_232735620.1 hypothetical protein -
  CWE29_RS12050 (CWE29_12050) - 2366172..2367176 (-) 1005 WP_100970589.1 DUF4767 domain-containing protein -
  CWE29_RS12055 (CWE29_12055) - 2367251..2367547 (-) 297 WP_002304106.1 peptidase -
  CWE29_RS12060 (CWE29_12060) - 2367540..2367833 (-) 294 WP_002328685.1 Mor transcription activator family protein -
  CWE29_RS12065 (CWE29_12065) - 2367979..2368530 (-) 552 WP_002322883.1 DUF1643 domain-containing protein -
  CWE29_RS12070 (CWE29_12070) - 2368704..2369003 (-) 300 WP_100970590.1 4'-phosphopantetheinyl transferase -
  CWE29_RS12075 (CWE29_12075) - 2369030..2369338 (-) 309 WP_002317115.1 DUF960 domain-containing protein -
  CWE29_RS12080 (CWE29_12080) - 2369553..2370725 (-) 1173 WP_002323245.1 IS256-like element IS1542 family transposase -
  CWE29_RS14135 - 2370749..2372137 (+) 1389 WP_232735621.1 hypothetical protein -
  CWE29_RS12095 (CWE29_12095) dfrG 2372516..2373013 (+) 498 WP_000868795.1 trimethoprim-resistant dihydrofolate reductase DfrG -
  CWE29_RS12100 (CWE29_12100) - 2373085..2375037 (-) 1953 WP_000163792.1 hypothetical protein -
  CWE29_RS12105 (CWE29_12105) - 2375044..2375280 (-) 237 WP_001159903.1 hypothetical protein -
  CWE29_RS12110 (CWE29_12110) - 2375811..2376317 (-) 507 WP_094940919.1 hypothetical protein -
  CWE29_RS12115 (CWE29_12115) - 2376483..2377436 (-) 954 WP_100970591.1 IS30 family transposase -
  CWE29_RS13820 - 2377519..2377680 (-) 162 WP_010726126.1 hypothetical protein -
  CWE29_RS12120 (CWE29_12120) rlmH 2377883..2378362 (-) 480 WP_002287841.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  CWE29_RS12125 (CWE29_12125) htrA 2378791..2380077 (+) 1287 WP_002325236.1 S1C family serine protease Regulator
  CWE29_RS12135 (CWE29_12135) ytpR 2380242..2380847 (-) 606 WP_002349782.1 YtpR family tRNA-binding protein -
  CWE29_RS12140 (CWE29_12140) - 2381052..2381516 (-) 465 WP_002287838.1 universal stress protein -
  CWE29_RS12145 (CWE29_12145) - 2381538..2381858 (-) 321 WP_002287837.1 thioredoxin family protein -
  CWE29_RS12150 (CWE29_12150) pepA 2382001..2383080 (-) 1080 WP_002351291.1 glutamyl aminopeptidase -
  CWE29_RS12155 (CWE29_12155) - 2383280..2383630 (+) 351 WP_002289862.1 PepSY domain-containing protein -
  CWE29_RS12160 (CWE29_12160) - 2383853..2384800 (+) 948 WP_002289860.1 glycosyltransferase family 2 protein -
  CWE29_RS12165 (CWE29_12165) pepV 2384857..2386275 (-) 1419 WP_002294157.1 dipeptidase PepV -
  CWE29_RS12170 (CWE29_12170) - 2386424..2387269 (+) 846 WP_002351294.1 NAD(P)H-hydrate dehydratase -
  CWE29_RS12175 (CWE29_12175) - 2387273..2387713 (+) 441 WP_002289317.1 acyl-CoA thioesterase -
  CWE29_RS12180 (CWE29_12180) - 2387763..2387957 (+) 195 WP_002315615.1 hypothetical protein -
  CWE29_RS12185 (CWE29_12185) - 2388093..2388779 (+) 687 WP_002289316.1 GntR family transcriptional regulator -
  CWE29_RS13950 - 2389244..2390670 (-) 1427 Protein_2229 IS3-like element ISEfa10 family transposase -
  CWE29_RS12200 (CWE29_12200) - 2390751..2391440 (-) 690 WP_002289315.1 hypothetical protein -
  CWE29_RS12205 (CWE29_12205) - 2391415..2393544 (-) 2130 WP_098188876.1 hydantoinase/oxoprolinase family protein -
  CWE29_RS12210 (CWE29_12210) - 2393592..2394929 (-) 1338 WP_100970592.1 citrate transporter -

Sequence

Protein


Download         Length: 428 a.a.        Molecular weight: 44827.81 Da        Isoelectric Point: 4.5419

>NTDB_id=258163 CWE29_RS12125 WP_002325236.1 2378791..2380077(+) (htrA) [Enterococcus faecium strain LS170308]
MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAMGTGNNSSTTSGNQNSAGETVVENVKVNVDSDITKAV
DKVQDAVVSVINLQSQNQSSGFGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKDG
TKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANSVTSGIISSLNRQVTSTNESNET
VNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKIASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGI
TMVDLSAVSTQQQEQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKVGDSVKITY
YEGSKEKTTTVELTIDQSALKQNQNSEN

Nucleotide


Download         Length: 1287 bp        

>NTDB_id=258163 CWE29_RS12125 WP_002325236.1 2378791..2380077(+) (htrA) [Enterococcus faecium strain LS170308]
ATGGATAGAAAAAATGTGACACCAAAGATGAAAAAAAATAAAAATAATAGCCTCTGGCGTAAGTTAGGTCTAGGGCTTGT
TGGAGGGATCGTTGGAGGATTACTGACAGCTGGTATTTTTTATGCAGCGATGGGCACTGGCAACAATAGTTCTACTACAA
GTGGCAATCAAAATTCTGCTGGTGAAACAGTTGTGGAAAACGTCAAAGTCAATGTAGATTCTGATATCACCAAAGCAGTA
GATAAAGTTCAAGACGCTGTCGTTTCTGTTATCAACTTGCAAAGTCAAAACCAATCAAGCGGTTTTGGCGGACTATTCGG
GCAGCAAGAAGAAAGTAGCAGCTCAGATGATAGTAATTTGGAAGCTTATAGTGAAGGAAGCGGCGTGATCTACAAAAAAG
ACGGGAATACTGCTTATGTAGTCACAAACAACCACGTAGTAGATGGCCAGCAAGGTCTTGAAGTCTTGATGAAAGACGGT
ACGAAGGTAAAAGCTGAATTAGTTGGAACAGATGCTTATTCAGATTTAGCGGTGTTGAAGATCAATTCAGACAAAGTCGA
AACAGTTGCCTCTTTTGGTGACTCAAGTGCTCTGAAGGTTGGTGAGCCTGCGATTGCGATTGGTTCCCCATTAGGATCTG
AATATGCGAATTCTGTCACTTCAGGAATCATTTCTTCCTTGAACCGACAAGTAACAAGTACAAATGAATCGAACGAAACA
GTCAATATCAATGCGATCCAAACAGATGCAGCAATCAACCCTGGAAATTCTGGTGGTCCTTTAGTGAATATCGAAGGACA
AGTTATCGGTATCAACTCAAGTAAGATCGCAAGTACTTCTGAATCTACTTCTAATGTAAGCGTAGAAGGAATGGGATTTG
CTATTCCAAGTAACGATGTAGTGAATATCATCAATCAACTGGAAAAAGACGGAAAAGTGACACGTCCAGCTCTAGGTATC
ACAATGGTCGACTTATCTGCCGTTTCTACTCAGCAACAAGAACAAATCTTGAAGATTCCTTCATCTGTAACTAACGGTGT
CATCGTGACAAATGTCCAAACAGCTACACCGGCTGAAAAAGCTGGATTGAAACAATACGACGTCATTACTAAGATCGATG
ACAAAGAAGTTTCTTCTGGTGTAGAACTTCAATCTGCTTTGTATAAGAAAAAAGTGGGAGATTCTGTCAAAATCACTTAC
TATGAAGGCTCGAAAGAAAAAACAACTACTGTTGAATTGACGATCGATCAATCGGCATTAAAACAAAATCAAAATAGTGA
AAATTAA

Domains

Predicted by InterproScan.

PDZ domain (PDZ_2)

(316-413)

Trypsin-like peptidase domain (Trypsin_2)

(126-271)

Peptidase family M50 (Peptidase_M50)

(10-416)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366TVN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus gordonii str. Challis substr. CH1

54.707

91.822

0.502
  htrA Streptococcus mutans UA159

59.887

82.71

0.495
  htrA Streptococcus mitis NCTC 12261

52.645

92.757

0.488
  htrA Streptococcus pneumoniae TIGR4

59.05

78.738

0.465
  htrA Streptococcus pneumoniae D39

59.05

78.738

0.465
  htrA Streptococcus pneumoniae Rx1

59.05

78.738

0.465
  htrA Streptococcus pneumoniae R6

59.05

78.738

0.465

Multiple sequence alignment