Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   CWQ20_RS01315 Genome accession   NZ_CP025029
Coordinates   212041..213084 (+) Length   347 a.a.
NCBI ID   WP_223295889.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain SGEHI2015-25     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 207041..218084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWQ20_RS01300 (CWQ20_01300) - 210638..211003 (+) 366 WP_000285373.1 DUF1033 family protein -
  CWQ20_RS01310 (CWQ20_01310) comYA 211176..212147 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  CWQ20_RS01315 (CWQ20_01315) comYB 212041..213084 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  CWQ20_RS01320 (CWQ20_01320) comGC/cglC 213081..213410 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  CWQ20_RS01325 (CWQ20_01325) comYD 213385..213798 (+) 414 WP_000244261.1 competence type IV pilus minor pilin ComGD Machinery gene
  CWQ20_RS01330 (CWQ20_01330) comGE 213770..214069 (+) 300 WP_000234804.1 competence type IV pilus minor pilin ComGE -
  CWQ20_RS01335 (CWQ20_01335) comGF 214023..214484 (+) 462 WP_001874812.1 competence type IV pilus minor pilin ComGF -
  CWQ20_RS01340 (CWQ20_01340) comGG 214462..214833 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  CWQ20_RS01345 (CWQ20_01345) comYH 214948..215922 (+) 975 WP_001008574.1 class I SAM-dependent methyltransferase Machinery gene
  CWQ20_RS01350 (CWQ20_01350) - 215954..217147 (+) 1194 WP_000047534.1 acetate kinase -
  CWQ20_RS01355 (CWQ20_01355) - 217299..217505 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  CWQ20_RS11110 (CWQ20_01360) - 217573..217701 (+) 129 WP_014959723.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39936.65 Da        Isoelectric Point: 9.9002

>NTDB_id=257419 CWQ20_RS01315 WP_223295889.1 212041..213084(+) (comYB) [Streptococcus agalactiae strain SGEHI2015-25]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRNYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=257419 CWQ20_RS01315 WP_223295889.1 212041..213084(+) (comYB) [Streptococcus agalactiae strain SGEHI2015-25]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGTATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGAATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTATTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(34-150)

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

62.17

98.271

0.611
  comYB Streptococcus mutans UA159

61.877

98.271

0.608
  comYB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

55.224

96.542

0.533
  comGB/cglB Streptococcus mitis SK321

54.627

96.542

0.527
  comGB/cglB Streptococcus pneumoniae Rx1

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae D39

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae R6

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

53.731

96.542

0.519
  comGB Lactococcus lactis subsp. cremoris KW2

50.148

97.118

0.487

Multiple sequence alignment