Detailed information NTG0256828

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CV098_RS10890
• Genome accession: NZ_CP024958
• Coordinates: 2231715..2232161 (-)
• Length: 148 a.a.
• NCBI ID: WP_032948129.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain G423
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2226715..2237161

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CV098_RS10855 (CV098_10855)	-	2226861..2227976 (-)	1116	Protein_2115	acyltransferase family protein	-
CV098_RS10860 (CV098_10860)	-	2227987..2228280 (+)	294	WP_226898169.1	pseudouridine synthase	-
CV098_RS10865 (CV098_10865)	-	2228319..2229128 (-)	810	WP_014570791.1	metal ABC transporter permease	-
CV098_RS10870 (CV098_10870)	-	2229121..2229858 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
CV098_RS10875 (CV098_10875)	-	2230035..2230877 (-)	843	WP_017865154.1	metal ABC transporter substrate-binding protein	-
CV098_RS10880 (CV098_10880)	-	2230874..2231311 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
CV098_RS10885 (CV098_10885)	comGG	2231392..2231676 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
CV098_RS10890 (CV098_10890)	comGF	2231715..2232161 (-)	447	WP_032948129.1	competence type IV pilus minor pilin ComGF	Machinery gene
CV098_RS10895 (CV098_10895)	comGE	2232124..2232420 (-)	297	WP_017865157.1	competence type IV pilus minor pilin ComGE	Machinery gene
CV098_RS10900 (CV098_10900)	comGD	2232392..2232823 (-)	432	WP_026138965.1	competence type IV pilus minor pilin ComGD	Machinery gene
CV098_RS10905 (CV098_10905)	comGC	2232783..2233118 (-)	336	WP_032948139.1	competence type IV pilus major pilin ComGC	Machinery gene
CV098_RS10910 (CV098_10910)	comGB	2233180..2234253 (-)	1074	WP_080613278.1	competence type IV pilus assembly protein ComGB	Machinery gene
CV098_RS10915 (CV098_10915)	comGA	2234147..2235085 (-)	939	WP_026138968.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=256828 CV098_RS10890 WP_032948129.1 2231715..2232161(-) (comGF) [Lactococcus lactis subsp. lactis strain G423]
MERKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=256828 CV098_RS10890 WP_032948129.1 2231715..2232161(-) (comGF) [Lactococcus lactis subsp. lactis strain G423]
ATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	42.336	92.568	0.392
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378