Detailed information NTG0256731

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CV702_RS10870
• Genome accession: NZ_CP024954
• Coordinates: 2244102..2244548 (-)
• Length: 148 a.a.
• NCBI ID: WP_032948129.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain F44
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2239102..2249548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CV702_RS10835 (CV702_10845)	-	2239248..2240363 (-)	1116	Protein_2124	acyltransferase family protein	-
CV702_RS10840 (CV702_10850)	-	2240374..2240667 (+)	294	WP_226898169.1	pseudouridine synthase	-
CV702_RS10845 (CV702_10855)	-	2240706..2241515 (-)	810	WP_014570791.1	metal ABC transporter permease	-
CV702_RS10850 (CV702_10860)	-	2241508..2242245 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
CV702_RS10855 (CV702_10865)	-	2242422..2243264 (-)	843	WP_017865154.1	metal ABC transporter substrate-binding protein	-
CV702_RS10860 (CV702_10870)	-	2243261..2243698 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
CV702_RS10865 (CV702_10875)	comGG	2243779..2244063 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
CV702_RS10870 (CV702_10880)	comGF	2244102..2244548 (-)	447	WP_032948129.1	competence type IV pilus minor pilin ComGF	Machinery gene
CV702_RS10875 (CV702_10885)	comGE	2244511..2244807 (-)	297	WP_017865157.1	competence type IV pilus minor pilin ComGE	Machinery gene
CV702_RS10880 (CV702_10890)	comGD	2244779..2245210 (-)	432	WP_026138965.1	competence type IV pilus minor pilin ComGD	Machinery gene
CV702_RS10885 (CV702_10895)	comGC	2245170..2245505 (-)	336	WP_032948139.1	competence type IV pilus major pilin ComGC	Machinery gene
CV702_RS10890 (CV702_10900)	comGB	2245567..2246640 (-)	1074	WP_080613278.1	competence type IV pilus assembly protein ComGB	Machinery gene
CV702_RS10895 (CV702_10905)	comGA	2246534..2247472 (-)	939	WP_026138968.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=256731 CV702_RS10870 WP_032948129.1 2244102..2244548(-) (comGF) [Lactococcus lactis subsp. lactis strain F44]
MERKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=256731 CV702_RS10870 WP_032948129.1 2244102..2244548(-) (comGF) [Lactococcus lactis subsp. lactis strain F44]
ATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	42.336	92.568	0.392
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378