Detailed information NTG0256261

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CVD07_RS12375
• Genome accession: NZ_CP024897
• Coordinates: 2537562..2538176 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain CN026
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2532562..2543176

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CVD07_RS12350 (CVD07_12395)	rpsT	2532865..2533131 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CVD07_RS12355 (CVD07_12400)	holA	2533148..2534189 (-)	1042	Protein_2397	DNA polymerase III subunit delta	-
CVD07_RS19855	-	2534229..2534380 (-)	152	Protein_2398	hypothetical protein	-
CVD07_RS12360 (CVD07_12405)	-	2534421..2534555 (+)	135	WP_003152870.1	YqzM family protein	-
CVD07_RS12365 (CVD07_12410)	comEC	2534574..2536925 (-)	2352	WP_100261899.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CVD07_RS12370 (CVD07_12415)	-	2536926..2537495 (-)	570	WP_003152868.1	ComE operon protein 2	-
CVD07_RS12375 (CVD07_12420)	comEA	2537562..2538176 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
CVD07_RS12380 (CVD07_12425)	comER	2538235..2539056 (+)	822	WP_014418420.1	late competence protein ComER	-
CVD07_RS12385 (CVD07_12430)	-	2539125..2539862 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
CVD07_RS12390 (CVD07_12435)	rsfS	2539859..2540215 (-)	357	WP_007408260.1	ribosome silencing factor	-
CVD07_RS12395 (CVD07_12440)	yqeK	2540233..2540793 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CVD07_RS12400 (CVD07_12445)	-	2540783..2541352 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CVD07_RS12405 (CVD07_12450)	yhbY	2541363..2541653 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CVD07_RS12410 (CVD07_12455)	aroE	2541647..2542489 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=256261 CVD07_RS12375 WP_014418419.1 2537562..2538176(-) (comEA) [Bacillus velezensis strain CN026]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=256261 CVD07_RS12375 WP_014418419.1 2537562..2538176(-) (comEA) [Bacillus velezensis strain CN026]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363