Detailed information NTG0254751

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CS547_RS12520
• Genome accession: NZ_CP024647
• Coordinates: 2551340..2551954 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain Lzh-5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2546340..2556954

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CS547_RS12495 (CS547_12485)	rpsT	2546643..2546909 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CS547_RS12500 (CS547_12490)	holA	2546926..2547967 (-)	1042	Protein_2386	DNA polymerase III subunit delta	-
CS547_RS20095	-	2548007..2548158 (-)	152	Protein_2387	hypothetical protein	-
CS547_RS12505 (CS547_12495)	-	2548199..2548333 (+)	135	WP_003152870.1	YqzM family protein	-
CS547_RS12510 (CS547_12500)	comEC	2548352..2550703 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CS547_RS12515 (CS547_12505)	-	2550704..2551273 (-)	570	WP_003152868.1	ComE operon protein 2	-
CS547_RS12520 (CS547_12510)	comEA	2551340..2551954 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
CS547_RS12525 (CS547_12515)	comER	2552013..2552834 (+)	822	WP_014418420.1	late competence protein ComER	-
CS547_RS12530 (CS547_12520)	-	2552903..2553640 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
CS547_RS12535 (CS547_12525)	rsfS	2553637..2553993 (-)	357	WP_007408260.1	ribosome silencing factor	-
CS547_RS12540 (CS547_12530)	yqeK	2554011..2554571 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CS547_RS12545 (CS547_12535)	-	2554561..2555130 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CS547_RS12550 (CS547_12540)	yhbY	2555141..2555431 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CS547_RS12555 (CS547_12545)	aroE	2555425..2556267 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=254751 CS547_RS12520 WP_014418419.1 2551340..2551954(-) (comEA) [Bacillus velezensis strain Lzh-5]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=254751 CS547_RS12520 WP_014418419.1 2551340..2551954(-) (comEA) [Bacillus velezensis strain Lzh-5]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363